ID   PLS3_PHYSA              Reviewed;          66 AA.
AC   F7UI86;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Phylloseptin-S3 {ECO:0000303|PubMed:23967105};
DE            Short=PLS-S3 {ECO:0000303|PubMed:23967105};
DE   Flags: Precursor;
OS   Phyllomedusa sauvagei (Sauvage's leaf frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=8395;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SYNTHESIS OF 47-65, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RC   TISSUE=Skin secretion;
RX   PubMed=23967105; DOI=10.1371/journal.pone.0070782;
RA   Raja Z., Andre S., Piesse C., Sereno D., Nicolas P., Foulon T., Oury B.,
RA   Ladram A.;
RT   "Structure, antimicrobial activities and mode of interaction with membranes
RT   of novel [corrected] phylloseptins from the painted-belly leaf frog,
RT   Phyllomedusa sauvagii.";
RL   PLoS ONE 8:E70782-E70782(2013).
CC   -!- FUNCTION: Antimicrobial peptide with activity against the Gram-positive
CC       S.pyogenes (MIC=12.5 uM), but not against all other bacteria tested
CC       (both Gram-positive and Gram-negative) (PubMed:23967105). Does not show
CC       activity against fungi, and against Leishmania species
CC       (PubMed:23967105). {ECO:0000269|PubMed:23967105}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is <7. Is active against E.coli ATCC25922 at pH 5 (MIC=50
CC         uM), whereas is not active at pH 7 (MIC>200 uM).
CC         {ECO:0000269|PubMed:23967105};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23967105}. Target
CC       cell membrane {ECO:0000305|PubMed:23967105}. Note=Forms a helical
CC       membrane channel in the target. {ECO:0000305|PubMed:23967105}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:23967105}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Phylloseptin subfamily. {ECO:0000305}.
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DR   EMBL; AM903079; CAP17492.1; -; mRNA.
DR   AlphaFoldDB; F7UI86; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR016322; FSAP.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
DR   PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Cleavage on pair of basic residues;
KW   Immunity; Innate immunity; Membrane; Secreted; Signal;
KW   Target cell membrane; Target membrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..46
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000449583"
FT   PEPTIDE         47..65
FT                   /note="Phylloseptin-S3"
FT                   /id="PRO_5003369566"
FT   REGION          25..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         65
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000250|UniProtKB:F7UI85"
SQ   SEQUENCE   66 AA;  7549 MW;  B24A694B78332AD6 CRC64;
     MAFLKKSLFL VLFLGLVSLS ICEEEKRETE EEEHDQEEDD KSEEKRFLSL IPHIVSGVAS
     LAIHFG