A76A_DROME
ID A76A_DROME Reviewed; 386 AA.
AC Q9VVW1; O46224;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Accessory gland protein Acp76A;
DE Flags: Precursor;
GN Name=Acp76A; ORFNames=CG3801;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAG38149.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Male accessory gland;
RX PubMed=9474779; DOI=10.1016/s0965-1748(97)00056-8;
RA Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W.,
RA Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K.;
RT "New genes for male accessory gland proteins in Drosophila melanogaster.";
RL Insect Biochem. Mol. Biol. 27:825-834(1997).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-386.
RC STRAIN=ZIM10C, ZIM12C, ZIM16C, ZIM18C, ZIM28C, ZIM30C, ZIM34C, ZIM53C,
RC ZIM5C, and ZIM7C;
RX PubMed=11102381; DOI=10.1093/genetics/156.4.1879;
RA Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G.;
RT "Molecular population genetics of male accessory gland proteins in
RT Drosophila.";
RL Genetics 156:1879-1888(2000).
CC -!- FUNCTION: Responsible for physiological and behavioral changes in mated
CC female flies. May play a role in accessory protein regulation and/or in
CC the coagulation of seminal fluid to form a mating plug.
CC {ECO:0000269|PubMed:9474779}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Main cells of accessory gland and seminal fluid.
CC {ECO:0000269|PubMed:9474779}.
CC -!- SIMILARITY: Belongs to the serpin family. {ECO:0000305}.
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DR EMBL; U90947; AAB96393.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF49196.1; -; Genomic_DNA.
DR EMBL; AY010627; AAG38149.1; -; Genomic_DNA.
DR EMBL; AY010628; AAG38150.1; -; Genomic_DNA.
DR EMBL; AY010629; AAG38151.1; -; Genomic_DNA.
DR EMBL; AY010630; AAG38152.1; -; Genomic_DNA.
DR EMBL; AY010631; AAG38153.1; -; Genomic_DNA.
DR EMBL; AY010632; AAG38154.1; -; Genomic_DNA.
DR EMBL; AY010633; AAG38155.1; -; Genomic_DNA.
DR EMBL; AY010634; AAG38156.1; -; Genomic_DNA.
DR EMBL; AY010635; AAG38157.1; -; Genomic_DNA.
DR EMBL; AY010636; AAG38158.1; -; Genomic_DNA.
DR RefSeq; NP_524153.1; NM_079429.2.
DR AlphaFoldDB; Q9VVW1; -.
DR SMR; Q9VVW1; -.
DR STRING; 7227.FBpp0074805; -.
DR GlyGen; Q9VVW1; 2 sites.
DR PaxDb; Q9VVW1; -.
DR EnsemblMetazoa; FBtr0075038; FBpp0074805; FBgn0015586.
DR GeneID; 40078; -.
DR KEGG; dme:Dmel_CG3801; -.
DR CTD; 40078; -.
DR FlyBase; FBgn0015586; Acp76A.
DR VEuPathDB; VectorBase:FBgn0015586; -.
DR eggNOG; KOG2392; Eukaryota.
DR OrthoDB; 1124079at2759; -.
DR PhylomeDB; Q9VVW1; -.
DR BioGRID-ORCS; 40078; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40078; -.
DR PRO; PR:Q9VVW1; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0015586; Expressed in male reproductive gland and 9 other tissues.
DR ExpressionAtlas; Q9VVW1; differential.
DR Genevisible; Q9VVW1; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0007610; P:behavior; IEA:UniProtKB-KW.
DR GO; GO:0010466; P:negative regulation of peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0019953; P:sexual reproduction; HEP:FlyBase.
DR Gene3D; 2.30.39.10; -; 1.
DR Gene3D; 3.30.497.10; -; 1.
DR InterPro; IPR023795; Serpin_CS.
DR InterPro; IPR023796; Serpin_dom.
DR InterPro; IPR000215; Serpin_fam.
DR InterPro; IPR036186; Serpin_sf.
DR InterPro; IPR042178; Serpin_sf_1.
DR InterPro; IPR042185; Serpin_sf_2.
DR PANTHER; PTHR11461; PTHR11461; 1.
DR Pfam; PF00079; Serpin; 1.
DR SMART; SM00093; SERPIN; 1.
DR SUPFAM; SSF56574; SSF56574; 1.
DR PROSITE; PS00284; SERPIN; 1.
PE 2: Evidence at transcript level;
KW Behavior; Glycoprotein; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000305"
FT CHAIN 23..386
FT /note="Accessory gland protein Acp76A"
FT /id="PRO_0000032528"
FT SITE 339..340
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 378
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 22
FT /note="Q -> H (in strain: ZIM5C, ZIM10C, ZIM16C, ZIM18C,
FT ZIM28C, ZIM30C, ZIM34C and ZIM53C)"
FT /evidence="ECO:0000305"
FT VARIANT 22
FT /note="Q -> P (in strain: ZIM7C and ZIM12C)"
FT /evidence="ECO:0000305"
FT VARIANT 332..337
FT /note="EVVDDI -> GKRNTR (in strain: ZIM28C)"
FT VARIANT 338
FT /note="D -> N (in strain: Canton-S and ZIM34C)"
FT VARIANT 359
FT /note="I -> V (in strain: Canton-S, ZIM12C and ZIM16C)"
FT VARIANT 381
FT /note="K -> M (in strain: ZIM5C and ZIM16C)"
FT /evidence="ECO:0000305"
FT CONFLICT 7
FT /note="I -> T (in Ref. 1; AAB96393)"
FT /evidence="ECO:0000305"
FT CONFLICT 64..65
FT /note="NN -> TI (in Ref. 4; AAG38149/AAG38150)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="Y -> S (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="K -> T (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="E -> A (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 165..167
FT /note="NAG -> YAA (in Ref. 4; AAG38149/AAG38150)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="A -> S (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="W -> C (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="N -> I (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="Y -> H (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="N -> Y (in Ref. 4; AAG38149/AAG38150/AAG38151/
FT AAG38152/AAG38153/AAG38154/AAG38155/AAG38156/AAG38157/
FT AAG38158)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 386 AA; 43967 MW; D2640A616B3F0FC8 CRC64;
MGNHQVIFLV LCTSLLFQNT IQQNVSFQLI REIDRYTPEN FVLSVLNIEM ILFEIHAAKA
VESNNDLERS LIINFGYSEA RQEVLDWGLR YKKASSAKFQ MANKVAVSQK LPLSQKLRLV
NEVLMTSAKK YDVTKDVRPS KLMDEWLSSH LDGVLANFVQ EKKLNAGENI VAISGMTVTP
LWASHFQSEI NRYFVNNPGT GYASKDPTCV PMMHSLSSFE TMSTDEAKGI YIPFSSANLG
MLILLPRKGV TCKDILDNLN NQINVEYNDH KDVHLLLPIF KEKFDYNIAK FFNGINIEDT
FKDSAFKSKA KIKINNFRVN HGIRFQPILR LEVVDDIDTG KTETFEVNRP FVFVIKDKIN
VYAVGRIENL DGLTDKVNCS KKYADL
