PLSB_COLP3
ID PLSB_COLP3 Reviewed; 811 AA.
AC Q48AL2;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Glycerol-3-phosphate acyltransferase {ECO:0000255|HAMAP-Rule:MF_00393};
DE Short=GPAT {ECO:0000255|HAMAP-Rule:MF_00393};
DE EC=2.3.1.15 {ECO:0000255|HAMAP-Rule:MF_00393};
GN Name=plsB {ECO:0000255|HAMAP-Rule:MF_00393}; OrderedLocusNames=CPS_0133;
OS Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio
OS psychroerythus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Colwelliaceae; Colwellia.
OX NCBI_TaxID=167879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=34H / ATCC BAA-681;
RX PubMed=16043709; DOI=10.1073/pnas.0504766102;
RA Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X.,
RA Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M.,
RA Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A.,
RA Zhou L., Davidsen T.M., Wu M., Huston A.L., Lewis M., Weaver B.,
RA Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.;
RT "The psychrophilic lifestyle as revealed by the genome sequence of
RT Colwellia psychrerythraea 34H through genomic and proteomic analyses.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00393};
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00393}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00393}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000255|HAMAP-
CC Rule:MF_00393}.
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DR EMBL; CP000083; AAZ27641.1; -; Genomic_DNA.
DR RefSeq; WP_011041008.1; NC_003910.7.
DR AlphaFoldDB; Q48AL2; -.
DR SMR; Q48AL2; -.
DR STRING; 167879.CPS_0133; -.
DR EnsemblBacteria; AAZ27641; AAZ27641; CPS_0133.
DR KEGG; cps:CPS_0133; -.
DR eggNOG; COG2937; Bacteria.
DR HOGENOM; CLU_015407_0_0_6; -.
DR OMA; EVIYVPC; -.
DR OrthoDB; 580383at2; -.
DR UniPathway; UPA00557; UER00612.
DR Proteomes; UP000000547; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR InterPro; IPR022284; GPAT/DHAPAT.
DR InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR InterPro; IPR045520; GPAT_C.
DR InterPro; IPR028354; GPAT_PlsB.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR PANTHER; PTHR12563; PTHR12563; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR Pfam; PF19277; GPAT_C; 1.
DR PIRSF; PIRSF500064; GPAT; 1.
DR PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR SMART; SM00563; PlsC; 1.
DR TIGRFAMs; TIGR03703; plsB; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW Phospholipid metabolism; Reference proteome; Transferase.
FT CHAIN 1..811
FT /note="Glycerol-3-phosphate acyltransferase"
FT /id="PRO_1000049429"
FT MOTIF 309..314
FT /note="HXXXXD motif"
SQ SEQUENCE 811 AA; 91530 MW; 8D4D8AA84F1B673B CRC64;
MLALRSFFYL LLKFPLKLLV RCKIITDSQN ITDQPNQPIF YIVRHQSASD LLALQSACKK
QNLPDPLGKV TINGESFNRT LCLAKSTPLC SWRKSSKTTA TAQGLALLNQ HVIDENIDAK
LIPANLIWGR TPTKERKNLN IGTLLADQES PNWLRKFFIV LFLGRDTLVR FSEAFSLRNI
SDNHGSDEAA AHKFLRVARF HFHRQTIAAK GPRLMHRKQM FTALFANPSV KRIISDEAKN
KKVSEAEIKK KALVMMNEIA GDYSVSWLRF GEIILHWLWK RLYSAIKVSN AKVLRKLAQD
GHEIIYVPCH RSHMDYLLLS YVILQEGLVM PRIAAGINLN FWPAGTIFRK GGAFFIRRSF
GGNRLYSTIF REYLGLLFER GYGVKYYTEG GRSRTGRVLA PKTGMLAMTI QSLLRGIDRP
LTLVPVYLGY EHVMEVGTYH KELSGSEKKG ESMFGVLKAI KSLRNYGNGY VNFGEPMNIN
EFLNKQVPDW KDSIDPIDPQ KPSWLTPTVN VLADQVMENI NKSAALNGVA LIALILHASK
NKALSKLELE TQLDFFLNIQ RQAPFSEQLT IPEETGAELL THVISLNKVT ITEDSFGSLV
SLSETANTEM RYYRNNILHT YVVPALVCRL LDKHSKINQD ELVIKVQNVT ALLKEDLYLY
QDSTHVEQQT LRVLTVLKEM EIAKQTKAGF WSLSDDVGLL SQVHAMAECI DESLQRLAII
TSLTSRLAPL SKRDLETKVV AIAKRLSVLN NINAPEFIDK RAQSTLIATI REQGYIDLDD
DGLLIASSTM AEIKATVINL VDIEVLQSIA R