PLSB_HAEIN
ID PLSB_HAEIN Reviewed; 810 AA.
AC P44857;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Glycerol-3-phosphate acyltransferase;
DE Short=GPAT;
DE EC=2.3.1.15;
GN Name=plsB; OrderedLocusNames=HI_0748;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000305}.
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DR EMBL; L42023; AAC22406.1; -; Genomic_DNA.
DR PIR; D64090; D64090.
DR RefSeq; NP_438907.1; NC_000907.1.
DR RefSeq; WP_005693149.1; NC_000907.1.
DR AlphaFoldDB; P44857; -.
DR SMR; P44857; -.
DR STRING; 71421.HI_0748; -.
DR DNASU; 949773; -.
DR EnsemblBacteria; AAC22406; AAC22406; HI_0748.
DR KEGG; hin:HI_0748; -.
DR PATRIC; fig|71421.8.peg.785; -.
DR eggNOG; COG2937; Bacteria.
DR HOGENOM; CLU_015407_0_0_6; -.
DR OMA; EVIYVPC; -.
DR PhylomeDB; P44857; -.
DR BioCyc; HINF71421:G1GJ1-786-MON; -.
DR UniPathway; UPA00557; UER00612.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006631; P:fatty acid metabolic process; IBA:GO_Central.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0008654; P:phospholipid biosynthetic process; IBA:GO_Central.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR InterPro; IPR022284; GPAT/DHAPAT.
DR InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR InterPro; IPR045520; GPAT_C.
DR InterPro; IPR028354; GPAT_PlsB.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR PANTHER; PTHR12563; PTHR12563; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR Pfam; PF19277; GPAT_C; 1.
DR PIRSF; PIRSF500064; GPAT; 1.
DR PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR SMART; SM00563; PlsC; 1.
DR TIGRFAMs; TIGR03703; plsB; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW Phospholipid metabolism; Reference proteome; Transferase.
FT CHAIN 1..810
FT /note="Glycerol-3-phosphate acyltransferase"
FT /id="PRO_0000195222"
FT MOTIF 306..311
FT /note="HXXXXD motif"
SQ SEQUENCE 810 AA; 92775 MW; DB8564BC3E2C370D CRC64;
MANFINMYRQ LLSLPLSALV KNNPIPANPI EELSLNIHQP IVYVLPYTSQ TDFVIFRRNC
LALGLPDPAE KNEINGVKLP RYVYLDEGRR IFKSKGAKDE TTTIFNKYLE LHRTSESLDV
QLIPVSVLWG RSPGQEDKSD LPNLRLLNGI QKTFAAIWFG RDTFVRFSQA VSLRYMVVEH
GSDEKIAQKL ARVAKMHFAK QRISATGPRL PNRQAMFNKL LQSEAIRRAI EDEAKSKNIS
IEKAQKEAYK ILDEIAADVS HSSLRAVDRF LRWLWNKLYS GINVQNSNRV RKLALEGHEI
VYVPCHRSHI DYLLLSYVLY HQGLVPPHIA AGINLNFWPI GRMFRSWGAF FIRRTFKGNR
LYSAIFREYL SELFHRGYSV EYFIEGGRSR TGRLLAPKTG MMSMTLQALQ HSQTRPISIV
PVYVGYEHVL EVDTYAKELR GAAKEKENAG LVLRVIKKLR NLGQGFVNFG EPITLSNYLS
QHFPDWKEQN HEEKPQWFTP AVNNISKQVM ININKAAAVN SMNLVGTALL SSRQRALSRE
QLLEQLSSYQ QLLQNVPYST DVVLPNVTPQ AMLEHVLALD RIGVLIEKDN FGEIVRLERS
SAVLMTYYRN NIQHLFVLPS LVASIILHYE AIQKDLLLDA IRKIYPFLQG ELFLHFNEDE
LNVQIHQIIN EFARQSVINS NDNFLSINKS KVRILQLWSA GMLEILQRYY ITVTILQKQP
AISRAELEKE SQLVAQRLSV LHGINAPEFF DKAVFSSFIA NLKEQRYFDE SGYTVLDKIE
ELASTLSHLI STEICLTVKG TIEKSEDLSS
