ID   PLSB_MYCTU              Reviewed;         789 AA.
AC   P9WI61; L0T9X9; O53207;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Glycerol-3-phosphate acyltransferase;
DE            Short=GPAT;
DE            EC=2.3.1.15;
GN   Name=plsB; Synonyms=plsB2; OrderedLocusNames=Rv2482c; ORFNames=MTV008.38c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC   -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC       diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC       may constitute the binding site for the phosphate moiety of the
CC       glycerol-3-phosphate. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45276.1; -; Genomic_DNA.
DR   PIR; A70868; A70868.
DR   RefSeq; NP_216998.1; NC_000962.3.
DR   RefSeq; WP_003917012.1; NZ_NVQJ01000067.1.
DR   AlphaFoldDB; P9WI61; -.
DR   SMR; P9WI61; -.
DR   STRING; 83332.Rv2482c; -.
DR   PaxDb; P9WI61; -.
DR   GeneID; 887848; -.
DR   KEGG; mtu:Rv2482c; -.
DR   TubercuList; Rv2482c; -.
DR   eggNOG; COG2937; Bacteria.
DR   OMA; EVIYVPC; -.
DR   PhylomeDB; P9WI61; -.
DR   UniPathway; UPA00557; UER00612.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006631; P:fatty acid metabolic process; IBA:GO_Central.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IBA:GO_Central.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IBA:GO_Central.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR   CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR   HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR   InterPro; IPR022284; GPAT/DHAPAT.
DR   InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR   InterPro; IPR045520; GPAT_C.
DR   InterPro; IPR028354; GPAT_PlsB.
DR   InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR   PANTHER; PTHR12563; PTHR12563; 1.
DR   Pfam; PF01553; Acyltransferase; 1.
DR   Pfam; PF19277; GPAT_C; 1.
DR   PIRSF; PIRSF500064; GPAT; 1.
DR   PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR   SMART; SM00563; PlsC; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Cell membrane; Lipid biosynthesis; Lipid metabolism;
KW   Membrane; Phospholipid biosynthesis; Phospholipid metabolism;
KW   Reference proteome; Transferase.
FT   CHAIN           1..789
FT                   /note="Glycerol-3-phosphate acyltransferase"
FT                   /id="PRO_0000195225"
FT   MOTIF           276..281
FT                   /note="HXXXXD motif"
SQ   SEQUENCE   789 AA;  88315 MW;  B78D7D8F8296EA22 CRC64;
     MTKPAADASA VLTAEDTLVL ASTATPVEME LIMGWLGQQR ARHPDSKFDI LKLPPRNAPP
     AALTALVEQL EPGFASSPQS GEDRSIVPVR VIWLPPADRS RAGKVAALLP GRDPYHPSQR
     QQRRILRTDP RRARVVAGES AKVSELRQQW RDTTVAEHKR DFAQFVSRRA LLALARAEYR
     ILGPQYKSPR LVKPEMLASA RFRAGLDRIP GATVEDAGKM LDELSTGWSQ VSVDLVSVLG
     RLASRGFDPE FDYDEYQVAA MRAALEAHPA VLLFSHRSYI DGVVVPVAMQ DNRLPPVHMF
     GGINLSFGLM GPLMRRSGMI FIRRNIGNDP LYKYVLKEYV GYVVEKRFNL SWSIEGTRSR
     TGKMLPPKLG LMSYVADAYL DGRSDDILLQ GVSICFDQLH EITEYAAYAR GAEKTPEGLR
     WLYNFIKAQG ERNFGKIYVR FPEAVSMRQY LGAPHGELTQ DPAAKRLALQ KMSFEVAWRI
     LQATPVTATG LVSALLLTTR GTALTLDQLH HTLQDSLDYL ERKQSPVSTS ALRLRSREGV
     RAAADALSNG HPVTRVDSGR EPVWYIAPDD EHAAAFYRNS VIHAFLETSI VELALAHAKH
     AEGDRVAAFW AQAMRLRDLL KFDFYFADST AFRANIAQEM AWHQDWEDHL GVGGNEIDAM
     LYAKRPLMSD AMLRVFFEAY EIVADVLRDA PPDIGPEELT ELALGLGRQF VAQGRVRSSE
     PVSTLLFATA RQVAVDQELI APAADLAERR VAFRRELRNI LRDFDYVEQI ARNQFVACEF
     KARQGRDRI