ID   PLSB_PASMU              Reviewed;         809 AA.
AC   Q9CLN7;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Glycerol-3-phosphate acyltransferase;
DE            Short=GPAT;
DE            EC=2.3.1.15;
GN   Name=plsB; OrderedLocusNames=PM1182;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC   -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC       diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC       may constitute the binding site for the phosphate moiety of the
CC       glycerol-3-phosphate. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000305}.
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DR   EMBL; AE004439; AAK03266.1; -; Genomic_DNA.
DR   RefSeq; WP_005751873.1; NC_002663.1.
DR   AlphaFoldDB; Q9CLN7; -.
DR   SMR; Q9CLN7; -.
DR   STRING; 747.DR93_785; -.
DR   EnsemblBacteria; AAK03266; AAK03266; PM1182.
DR   KEGG; pmu:PM1182; -.
DR   PATRIC; fig|272843.6.peg.1193; -.
DR   HOGENOM; CLU_015407_0_0_6; -.
DR   OMA; EVIYVPC; -.
DR   UniPathway; UPA00557; UER00612.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR   HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR   InterPro; IPR022284; GPAT/DHAPAT.
DR   InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR   InterPro; IPR045520; GPAT_C.
DR   InterPro; IPR028354; GPAT_PlsB.
DR   InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR   PANTHER; PTHR12563; PTHR12563; 1.
DR   Pfam; PF01553; Acyltransferase; 1.
DR   Pfam; PF19277; GPAT_C; 1.
DR   PIRSF; PIRSF500064; GPAT; 1.
DR   PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR   SMART; SM00563; PlsC; 1.
DR   TIGRFAMs; TIGR03703; plsB; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW   Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW   Phospholipid metabolism; Reference proteome; Transferase.
FT   CHAIN           1..809
FT                   /note="Glycerol-3-phosphate acyltransferase"
FT                   /id="PRO_0000195226"
FT   MOTIF           306..311
FT                   /note="HXXXXD motif"
SQ   SEQUENCE   809 AA;  92674 MW;  63CFE636A090382D CRC64;
     MFSLLNMYRK VLELPLSFLV KNNPIPHHPI EELKLDPTQP MVYVLPYTSQ TDLVIFRKNC
     LSVGLPDPLE KNEIEGKSLP RFVFLDEGRR FFKSKGAKEE TIQVFNQYLE LHHQRSDLDV
     QIIPVSVLWG RSPGHEKQSG LPQLRLLSGL QKFIAAVWFG RDTFVRFSQA VSLRYMVNEH
     GSDQAISHKL ARVAKIHFSK QRISATGPRL PNREAMFNKL LASPTILDAI QDEAKAKNIS
     EEKATQEAKK ILDEIAANVN YEGLRVADRF LRWLWNKLYQ GIDVQNADRV RKLALDGHEI
     VYVPCHRSHI DYLLLSYVLY HQGLVPPHIA AGINLNFWPV GGMFRRGGAF FIRRTFKGNR
     LYSTIFREYL AELFHRGYSV EYFIEGGRSR TGRLLAPKTG MMSMTLQALL QGQTRPISVV
     PVYVGYEHVL EVDTYAKELR GAAKEKENAG LVLRVIKKLR NLGKGYVNFG EPITLSNYLN
     QHYPEWKENT QAEERAKWFN QAVDSISHQV MVNINKAAAV NAMNLTGTAL LSSRQRALSR
     EQLLEQLESY QQFLQNVPYS QDIVVPTESP EKMLEHILQL ERVGIIVEKD NFGEIVRLER
     NSAVLMTYYR NNIQHLFVLP SLVASIVLHY EAIQKPLVLE AVSKIYPFLK SELFLSFSYE
     ELQDRVNHII TEFERQEVIK CNANMLSINK PRVRMLQLWS SGVREILQRY YITVSILEKD
     PATPRASLEK DSQSVAQRLS VLHGINAPEF FDKAVFSAFI ASLKQEGYFN EEGIANTEKL
     NEITEILNQV ISTEVCLTIK GAIAKIEEE