PLSB_SHELP
ID PLSB_SHELP Reviewed; 807 AA.
AC A3QIU8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Glycerol-3-phosphate acyltransferase {ECO:0000255|HAMAP-Rule:MF_00393};
DE Short=GPAT {ECO:0000255|HAMAP-Rule:MF_00393};
DE EC=2.3.1.15 {ECO:0000255|HAMAP-Rule:MF_00393};
GN Name=plsB {ECO:0000255|HAMAP-Rule:MF_00393}; OrderedLocusNames=Shew_3530;
OS Shewanella loihica (strain ATCC BAA-1088 / PV-4).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=323850;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1088 / PV-4;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G.,
RA Fredrickson J., Tiedje J., Richardson P.;
RT "Complete sequence of Shewanella loihica PV-4.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00393};
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00393}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00393}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000255|HAMAP-
CC Rule:MF_00393}.
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DR EMBL; CP000606; ABO25396.1; -; Genomic_DNA.
DR RefSeq; WP_011867325.1; NC_009092.1.
DR AlphaFoldDB; A3QIU8; -.
DR SMR; A3QIU8; -.
DR STRING; 323850.Shew_3530; -.
DR EnsemblBacteria; ABO25396; ABO25396; Shew_3530.
DR KEGG; slo:Shew_3530; -.
DR eggNOG; COG2937; Bacteria.
DR HOGENOM; CLU_015407_0_0_6; -.
DR OMA; EVIYVPC; -.
DR OrthoDB; 580383at2; -.
DR UniPathway; UPA00557; UER00612.
DR Proteomes; UP000001558; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR InterPro; IPR022284; GPAT/DHAPAT.
DR InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR InterPro; IPR045520; GPAT_C.
DR InterPro; IPR028354; GPAT_PlsB.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR PANTHER; PTHR12563; PTHR12563; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR Pfam; PF19277; GPAT_C; 1.
DR PIRSF; PIRSF500064; GPAT; 1.
DR PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR SMART; SM00563; PlsC; 1.
DR TIGRFAMs; TIGR03703; plsB; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW Phospholipid metabolism; Reference proteome; Transferase.
FT CHAIN 1..807
FT /note="Glycerol-3-phosphate acyltransferase"
FT /id="PRO_1000049458"
FT MOTIF 308..313
FT /note="HXXXXD motif"
SQ SEQUENCE 807 AA; 91491 MW; DD866D7D63DCD02B CRC64;
MSKQDSLWFK SLRWIQKKLV HTIVVPQDPF ADLNLDSDRP LVYVMKTESI SDIAALSEVT
DRLGLPSPYQ PLDVEGEQAP RVVCVEGAKP LLGEREGNEF YLESFSRLLS AHKKHPELDI
QLVPVSLYWG RTPGKEDDSM KAAVLERESP TWLRKCLMII FLGRHNFVQF SNAMSLRYMA
DEHGTDKRIA QKLARVARVH FSRQRKVMTG PVLPNRQNLF HALLKSESMK KAILEEAQSK
KISEAKARET AMEYLDEIAA DYSDSLVRIA ERFLTWLWNK LYKGINIKGA EQIRQLHHDG
HEIVYVPCHR SHMDYLLLSY ILYYQGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFR
GNKLYTAVFR EYLDQLFAKG YSVEYFTEGG RSRTGRLLAP KTGMIAMTLN SVLRGVERPV
TLVPVYLGYD HVMEVATYHK ELSGKKKQKE SVWQVFGAIR KLGNFGQGYV NFGEPITLHS
YLNEQVPEWR EELAQDPEQK PSWLTPVVNT LANRVMTRIN DAAAVSSITL TSLVLLASEQ
NALQRSQLEK QLDLYLSLLK SVPYTGYTSV VEGDGAKLIA QGLELKKLKL DSDPLGDIIS
IDESLAIAMT YYRNNIIHLL VIPSLIASCL TRQDECSRDE IIATVKDFYP LLQAELFMGI
DNLETYVNQL LDQLLAEGLI DEDKHFSVKP DGLNQLLLLA GTISETMQRY AIIFNLLAAS
PKIERSELES DSHQLAQRLG ALHGITAPEF YDKKLYGTLS VKLKELGYLA DDSHSEDVQR
IRTRANSLLR SSVRQTIVDS VAAEHQA
