ID   PLSB_VIBA3              Reviewed;         807 AA.
AC   B7VM97;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Glycerol-3-phosphate acyltransferase {ECO:0000255|HAMAP-Rule:MF_00393};
DE            Short=GPAT {ECO:0000255|HAMAP-Rule:MF_00393};
DE            EC=2.3.1.15 {ECO:0000255|HAMAP-Rule:MF_00393};
GN   Name=plsB {ECO:0000255|HAMAP-Rule:MF_00393}; OrderedLocusNames=VS_3009;
OS   Vibrio atlanticus (strain LGP32) (Vibrio splendidus (strain Mel32)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=575788;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LGP32;
RA   Mazel D., Le Roux F.;
RT   "Vibrio splendidus str. LGP32 complete genome.";
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00393};
CC   -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC       diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00393}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00393}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00393}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_00393}.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC       may constitute the binding site for the phosphate moiety of the
CC       glycerol-3-phosphate. {ECO:0000255|HAMAP-Rule:MF_00393}.
CC   -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000255|HAMAP-
CC       Rule:MF_00393}.
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DR   EMBL; FM954972; CAV20287.1; -; Genomic_DNA.
DR   RefSeq; WP_012605022.1; NC_011753.2.
DR   AlphaFoldDB; B7VM97; -.
DR   SMR; B7VM97; -.
DR   STRING; 575788.VS_3009; -.
DR   PRIDE; B7VM97; -.
DR   EnsemblBacteria; CAV20287; CAV20287; VS_3009.
DR   KEGG; vsp:VS_3009; -.
DR   PATRIC; fig|575788.5.peg.4199; -.
DR   eggNOG; COG2937; Bacteria.
DR   HOGENOM; CLU_015407_0_0_6; -.
DR   OMA; EVIYVPC; -.
DR   OrthoDB; 580383at2; -.
DR   UniPathway; UPA00557; UER00612.
DR   Proteomes; UP000009100; Chromosome 1.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR   HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR   InterPro; IPR022284; GPAT/DHAPAT.
DR   InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR   InterPro; IPR045520; GPAT_C.
DR   InterPro; IPR028354; GPAT_PlsB.
DR   InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR   PANTHER; PTHR12563; PTHR12563; 1.
DR   Pfam; PF01553; Acyltransferase; 1.
DR   Pfam; PF19277; GPAT_C; 1.
DR   PIRSF; PIRSF500064; GPAT; 1.
DR   PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR   SMART; SM00563; PlsC; 1.
DR   TIGRFAMs; TIGR03703; plsB; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW   Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW   Phospholipid metabolism; Reference proteome; Transferase.
FT   CHAIN           1..807
FT                   /note="Glycerol-3-phosphate acyltransferase"
FT                   /id="PRO_1000192411"
FT   MOTIF           305..310
FT                   /note="HXXXXD motif"
SQ   SEQUENCE   807 AA;  90758 MW;  BEB5A255B81CC15A CRC64;
     MSSGQSFSRS LMKLPLSLLV KSTSIPSNPV EDLNIDLSKP IVYALPFRSS VDILTLQKHA
     LELGLPDPLS KLEINGKSLQ RYVFISSRKT LLQDDDYVPS SSIEVFSELL SLHAEDSELD
     VQVIPATVLW GRKPGKENNQ KPYLQAMNGL EKSKAVLLAG RDCLVRFSPV VSLRYMANSH
     GTDSTIAHKL ARVARIHFSR QKLAASGPNL PSRQALFDRL LKSEAIKKAI EDEAESKNIS
     IEKASKEAQD IMDEIAANFS YSLIKRGEKI LGWLWNKLYQ GLHISNASTV RKLAQDGHEI
     VYVPCHRSHM DYLLLSYVLY HEGMVPPHIA AGINLNFFPA GPIFRHGGAF FIRRSFKGNK
     LYSTIFREYL AELFAKGYSV EYFSEGGRSR TGRLLQAKTG MLAMTIQAML RGMNRPVTLV
     PVYIGYEHVM EVATYAKELR GKRKEKENAS LVIRTIRKLR NFGKGYVNFG EPIQLNQYLN
     EHAPEWTKDI DPMGTSKPQW MNPVVNDLAT KMMTHINDAA ATNALTLCAT ALLASRQRAL
     SRDSLVSQIN CYLSLLKNVP YSDTFTVPKD SAEDLVKHAE SLNKFLIESD TMGDIISLDR
     HQSILMTYYR NNIIHLFALP SLIAQMTIRQ HGLSIDAIQE NVAAIYPFLK KELFLSYDED
     QLESVVANII DELVGQGMLV VSNNQVTINQ SNSQALMLLG RTISETLQRY SIALNLLAEN
     PDLDKSDLEQ KSQDIAQRLG RLHGINAPEF FDKGVFASMF ATLKQQQYLD NDGNCDLEKT
     QQFAKLLYSM LYPEVRLTIQ ESIHQAE