PLSB_VIBPA
ID PLSB_VIBPA Reviewed; 808 AA.
AC Q87KN0;
DT 30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2003, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Glycerol-3-phosphate acyltransferase {ECO:0000255|HAMAP-Rule:MF_00393};
DE Short=GPAT {ECO:0000255|HAMAP-Rule:MF_00393};
DE EC=2.3.1.15 {ECO:0000255|HAMAP-Rule:MF_00393};
GN Name=plsB {ECO:0000255|HAMAP-Rule:MF_00393}; OrderedLocusNames=VP2947;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00393};
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00393}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_00393}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000255|HAMAP-Rule:MF_00393}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000255|HAMAP-
CC Rule:MF_00393}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000031; BAC61210.1; -; Genomic_DNA.
DR RefSeq; NP_799326.1; NC_004603.1.
DR RefSeq; WP_005460370.1; NC_004603.1.
DR AlphaFoldDB; Q87KN0; -.
DR SMR; Q87KN0; -.
DR STRING; 223926.28807973; -.
DR EnsemblBacteria; BAC61210; BAC61210; BAC61210.
DR GeneID; 1190533; -.
DR KEGG; vpa:VP2947; -.
DR PATRIC; fig|223926.6.peg.2836; -.
DR eggNOG; COG2937; Bacteria.
DR HOGENOM; CLU_015407_0_0_6; -.
DR OMA; EVIYVPC; -.
DR UniPathway; UPA00557; UER00612.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR InterPro; IPR022284; GPAT/DHAPAT.
DR InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR InterPro; IPR045520; GPAT_C.
DR InterPro; IPR028354; GPAT_PlsB.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR PANTHER; PTHR12563; PTHR12563; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR Pfam; PF19277; GPAT_C; 1.
DR PIRSF; PIRSF500064; GPAT; 1.
DR PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR SMART; SM00563; PlsC; 1.
DR TIGRFAMs; TIGR03703; plsB; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW Phospholipid metabolism; Reference proteome; Transferase.
FT CHAIN 1..808
FT /note="Glycerol-3-phosphate acyltransferase"
FT /id="PRO_0000195236"
FT MOTIF 306..311
FT /note="HXXXXD motif"
SQ SEQUENCE 808 AA; 91143 MW; 96E9BAEA6DCE8B61 CRC64;
MSSGQSFSRS LLKLPLSVMV KGTTIPSNPI DDLNIDLTKP IVYALPFRSN VDLLTLQKQA
MSLGLPDPLS PLEINGKTLN RFVFIASRPT VMGNDNDIPT DSVSLFTELL ELHKLDSELD
VQMIPATVLW GRKPGKEESH RPYLQPMNGP QKAKAVMAAG RDCLVRFSPV VSLRYMADSH
GTDSAIAHKL ARVARIHFSR QKLAASGPNL PQRQVLFARL LKSPAIEQAI EDEAKSKDIS
IEKARKEAHD IMDEIAADFS YGLVKNGDRI LSWLWTKLYQ GLHINNASTV RRLAQDGHEI
VYVPCHRSHM DYLLLSYVLY HEGMVPPHIA AGINLNFFPA GPIFRRGGAF FIRRSFKGNK
LYSTIFREYL AELFAKGYSV EYFSEGGRSR TGRLLQAKTG MLAMTIQAML RGLNRPVTLV
PVYIGYEHVM EVGTYAKELR GKRKEKENAG LVLRTLRKLR NFGLGYVNFG EPIQLNQYLN
EHAPEWTKDI DSMGGSKPQW MNPVVNELAN KMMTHINDAA AANALTLCAT ALLASRQRAL
SRDSLINQIE CYLKLLKNNP YSSTSTIPTE SAEELVDHAI SLDKFVIETD SMGDIISLDR
SQSILMTYYR NNIIHLFALP SLIAQMIIRQ RNLTVEKIQE NVAQIYPFLK KELFLSYQEE
DLNDLVVKTL NEFAEQKMIC LDGNKLEINQ SNNQPLVLLG RTITETLQRY SIAMNLLVAY
PELGKSDLEQ KSQDIAQRLG RLHGINAPEF FDKGVFTAMF NTLKQQEYLD SDGNCDKKKT
QKFAKLLFTL LYPEVKLTIE ESIHQLQA
