ID   PLSB_VIBVU              Reviewed;         809 AA.
AC   Q8DD48;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Glycerol-3-phosphate acyltransferase {ECO:0000255|HAMAP-Rule:MF_00393};
DE            Short=GPAT {ECO:0000255|HAMAP-Rule:MF_00393};
DE            EC=2.3.1.15 {ECO:0000255|HAMAP-Rule:MF_00393};
GN   Name=plsB {ECO:0000255|HAMAP-Rule:MF_00393}; OrderedLocusNames=VV1_1165;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC         phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00393};
CC   -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC       diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00393}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00393}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00393}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_00393}.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC       may constitute the binding site for the phosphate moiety of the
CC       glycerol-3-phosphate. {ECO:0000255|HAMAP-Rule:MF_00393}.
CC   -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000255|HAMAP-
CC       Rule:MF_00393}.
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DR   EMBL; AE016795; AAO09636.1; -; Genomic_DNA.
DR   RefSeq; WP_011079175.1; NC_004459.3.
DR   AlphaFoldDB; Q8DD48; -.
DR   SMR; Q8DD48; -.
DR   EnsemblBacteria; AAO09636; AAO09636; VV1_1165.
DR   KEGG; vvu:VV1_1165; -.
DR   HOGENOM; CLU_015407_0_0_6; -.
DR   OMA; EVIYVPC; -.
DR   UniPathway; UPA00557; UER00612.
DR   Proteomes; UP000002275; Chromosome 1.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR   HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR   InterPro; IPR022284; GPAT/DHAPAT.
DR   InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR   InterPro; IPR045520; GPAT_C.
DR   InterPro; IPR028354; GPAT_PlsB.
DR   InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR   PANTHER; PTHR12563; PTHR12563; 1.
DR   Pfam; PF01553; Acyltransferase; 1.
DR   Pfam; PF19277; GPAT_C; 1.
DR   PIRSF; PIRSF500064; GPAT; 1.
DR   PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR   SMART; SM00563; PlsC; 1.
DR   TIGRFAMs; TIGR03703; plsB; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cell inner membrane; Cell membrane; Lipid biosynthesis;
KW   Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW   Phospholipid metabolism; Transferase.
FT   CHAIN           1..809
FT                   /note="Glycerol-3-phosphate acyltransferase"
FT                   /id="PRO_0000195237"
FT   MOTIF           306..311
FT                   /note="HXXXXD motif"
SQ   SEQUENCE   809 AA;  90596 MW;  0E04FF0B4980DCDC CRC64;
     MSSGQSFSHS LLKLPLSALV KGTAIPSNPI DDHHIDINKP IVYALPFRSA VDLLTLQKHA
     LELGLPDPLS PLEIHGKSLK RYVFIASRPT LVQSDNDVPS DSIALFSDLL ALHAEDSELD
     VQVIPATVLW GRKPGKEGNN KPYLQAMNGL QKAKAVITAG RDCLVRFSPV VSLRYMAQSH
     GTDSSIAHKL ARVARIHFSR QKLAASGPDL PSRQVLFARL MKSPAIEQAI EEEAKNKNIS
     MEKARKEAQD IMDEIAADFS YSLVKQGDRL LGWLWNKLYQ GLNINNAATV RRLAQDGHEI
     VYVPCHRSHM DYLLLSYVLY HEGMVPPHIA AGINLNFFPA GPIFRRGGAF FIRRSFKGNR
     LYSTIFREYL AELFAKGYSV EYFSEGGRSR TGRLLPAKTG MLAMTIQAML RGLNRPVTLV
     PVYIGYEHVM EVATYAKELR GKRKEKENAG LVLRTLRKLR NFGLGYVNFG EPIPLNQYLN
     EHAPEWTKDI DPMGASRPQW INPVVNQLAN KMMTHINDAA AANALTLCAT ALLASRQRAL
     SKDSLIHQIE CYLQLLKNVP YSKTYTVPSE SAEALVEHAI SLDKFVIETD TMGDIISLDR
     NQSILMTYYR NNIIHLFALP SLIAQMIIRQ ENLTVSQIQQ QVAEIYPFLK AELFLSHKEE
     ELDELVVKVL NELVSQDLIS LKEDKVAKNQ ANTLTLVLLG RTISETLQRY SIAFNLLVSN
     PELAKADLEQ KSQDIAQRLT RLHGINAPEY FDKGVFASLF STLKQQGYLD SDGNCDGEKT
     AQFATLLYAL LYPEVKLTIE ESVFQLKSA