ASXL3_MOUSE
ID ASXL3_MOUSE Reviewed; 2259 AA.
AC Q8C4A5; Q8C380;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 3.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative Polycomb group protein ASXL3;
DE AltName: Full=Additional sex combs-like protein 3;
GN Name=Asxl3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-961 AND 2090-2259 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION.
RX PubMed=16412590; DOI=10.1016/j.gene.2005.10.033;
RA Fisher C.L., Randazzo F., Humphries R.K., Brock H.W.;
RT "Characterization of Asxl1, a murine homolog of Additional sex combs, and
RT analysis of the Asx-like gene family.";
RL Gene 369:109-118(2006).
CC -!- FUNCTION: Putative Polycomb group (PcG) protein. PcG proteins act by
CC forming multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. PcG proteins are not required to initiate repression, but
CC to maintain it during later stages of development. They probably act
CC via methylation of histones, rendering chromatin heritably changed in
CC its expressibility (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C4A5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C4A5-2; Sequence=VSP_031720;
CC -!- SIMILARITY: Belongs to the Asx family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC103351; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC163208; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK082659; BAC38564.2; -; mRNA.
DR EMBL; AK086668; BAC39715.1; -; mRNA.
DR CCDS; CCDS50234.1; -. [Q8C4A5-2]
DR RefSeq; NP_001161249.1; NM_001167777.1. [Q8C4A5-2]
DR RefSeq; XP_011245170.1; XM_011246868.1. [Q8C4A5-2]
DR RefSeq; XP_017173342.1; XM_017317853.1.
DR AlphaFoldDB; Q8C4A5; -.
DR SMR; Q8C4A5; -.
DR BioGRID; 229278; 2.
DR STRING; 10090.ENSMUSP00000095260; -.
DR iPTMnet; Q8C4A5; -.
DR PhosphoSitePlus; Q8C4A5; -.
DR PaxDb; Q8C4A5; -.
DR PRIDE; Q8C4A5; -.
DR Antibodypedia; 82041; 3 antibodies from 1 providers.
DR Ensembl; ENSMUST00000097655; ENSMUSP00000095260; ENSMUSG00000045215. [Q8C4A5-2]
DR Ensembl; ENSMUST00000120223; ENSMUSP00000112793; ENSMUSG00000045215. [Q8C4A5-2]
DR GeneID; 211961; -.
DR KEGG; mmu:211961; -.
DR UCSC; uc008efm.2; mouse. [Q8C4A5-1]
DR CTD; 80816; -.
DR MGI; MGI:2685175; Asxl3.
DR VEuPathDB; HostDB:ENSMUSG00000045215; -.
DR eggNOG; ENOG502QWPH; Eukaryota.
DR GeneTree; ENSGT00520000055578; -.
DR HOGENOM; CLU_001823_0_0_1; -.
DR InParanoid; Q8C4A5; -.
DR OMA; MFTGNML; -.
DR OrthoDB; 53757at2759; -.
DR TreeFam; TF328464; -.
DR BioGRID-ORCS; 211961; 2 hits in 77 CRISPR screens.
DR PRO; PR:Q8C4A5; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q8C4A5; protein.
DR Bgee; ENSMUSG00000045215; Expressed in superior cervical ganglion and 157 other tissues.
DR ExpressionAtlas; Q8C4A5; baseline and differential.
DR Genevisible; Q8C4A5; MM.
DR GO; GO:0035517; C:PR-DUB complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042975; F:peroxisome proliferator activated receptor binding; IBA:GO_Central.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0051055; P:negative regulation of lipid biosynthetic process; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR026905; ASX-like_PHD.
DR InterPro; IPR024811; ASX/ASX-like.
DR InterPro; IPR028020; ASX_DEUBAD_dom.
DR InterPro; IPR024818; ASXL3.
DR InterPro; IPR007759; Asxl_HARE-HTH.
DR InterPro; IPR044867; DEUBAD_dom.
DR PANTHER; PTHR13578; PTHR13578; 1.
DR PANTHER; PTHR13578:SF18; PTHR13578:SF18; 1.
DR Pfam; PF13919; ASXH; 1.
DR Pfam; PF05066; HARE-HTH; 1.
DR Pfam; PF13922; PHD_3; 1.
DR PROSITE; PS51916; DEUBAD; 1.
DR PROSITE; PS51913; HTH_HARE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Metal-binding; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..2259
FT /note="Putative Polycomb group protein ASXL3"
FT /id="PRO_0000320671"
FT DOMAIN 10..83
FT /note="HTH HARE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01261"
FT DOMAIN 253..362
FT /note="DEUBAD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01264"
FT ZN_FING 2221..2258
FT /note="PHD-type; atypical"
FT REGION 181..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 607..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 703..810
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 857..1012
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1025..1049
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1126..1151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1433..1462
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1614..1643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1687..1719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1993..2075
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 377..399
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 703..786
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 920..946
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 947..984
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1029..1049
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1126..1149
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1433..1455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1698..1719
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2020..2060
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..55
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031720"
SQ SEQUENCE 2259 AA; 243673 MW; D1968675ABC6704B CRC64;
MKDKRKKKDR TWAEAARLAL EKHPNSPMTA KQILEVIQKE GLKETGTSPL ACLNAMLHTN
TRVGDGTFFK IPGKSGLYAL RKEESSCPVD GTLDLVVDPD LDGAEMAEAS ANGEENRVCT
KQVTDEVSST RDCSLTNTAV QSKLVSSFQQ HTKKALKQAL RQQQKRRNGV SMMVNKTVPR
VVLTPLKVSD EQSDSPSGSE SKNGEADSSD KEMKHGQKSP TGKQTSQHLK RLKKSGLGHL
KWTKAEDIDI ETPGSILVNT NLRALINKHT FASFPQHFQQ YLLLLLPEVD RQMGSDGILR
LSTSALNNEF FAYAAQGWKQ RLAEGEFTPE MQLRIRQEIE KEKKTEPWKE KFFERFYGER
SGMSREESIK LTSGPNHEGA EGSSSHGDSG IPGPSAQNAL EEQQPKILKS SASLEPDFCT
TVCPMLEVPV KDVMTESETE DIFIPEESVI QEEVAEEVET SIYECQDEHL KTIPAFSEES
ESPATPCEEP QVAAPEDSLE SCVVMNDILH TLPHIEVKIV EKLECPQEEM SVVIDQLEIC
DSLLPCPSSV THILDVEQKE QETTIETSAM ALREGPSSLE SQLPNEGIAV DMELQSDPEE
QLSENACISE TSFSSESPEG ACASLPSPGG ETQSTSEESC TPASLETTFC SEVSSTENTD
KYNQRNPTGE SLHASLVSEV SPLATSPEIS EASLMSNLPL TSEASPVSNL PLTSEASPMS
DLPPTSETSS ESSMPLTSET PFVSSLPIPA ETSPISNSSV NERMVHQQRK SPSGSEEANS
PQKEEPSIPT KPLGESLVSH PKPLSTIPEP INMSSAMVPE ALPPEGLHSQ TLSQEPCNAH
VEMEKLYASS IPELPSSEMT KVKNHSVLQR PEKKGLSAPL EVPVFSEETE TKGIELPPAK
LQDKQYAPSV DKATFLEGSR NKIHKQSSTL NRLETSHTSK VSEPSKSPDG IRNDNRESEI
SKRKTVEHSF GICKEKRARI EDDQSARSLA SSSPPEKEQP PREEPRVPPL KIQLSKIGPP
FIIKSQPVSK AESRASTSTS VSSGRNTGAR TLADIKARAQ QARAQREAAA AAAVAAAASI
VSGAMGSPGE GGKARTLAHI KEQTKAKLFA KHQSRAHLFQ TSKETRLPSV STKEDSLNME
ASPTPETKME GSTGVIIINP NCRSPSSKPT HIREITTVLQ QPLNPPQIPE TATDLSVHSS
DDNIPVSHLT EKIVSSTSSE NSSVPILHNK SPINPIPMSV CSTAMSGAIK EHPFVSPVDK
SSVLMSVDSA NSTISACNIS MLKSIQGSDA PCIALVPKCI NRTPIPAAPE GTGQSNSMDG
KALLVPSSKA ANVISNQYTS VPAPTIASNL PNHLCTSSVL IPPTGINNRF VSEKIAMPGS
EEQAAVSIGA TMRTALSCGD SVAVTDSLVP RSPIAMFAGN MLTANSYNCP PKLSGENLDN
NSGPLNRTDN SEKPQQPAGG FVPATINRSI PCKVIVDHST TLTSNLSLTV SIESGDSSLD
SQTRSVRTDV SIQPVACPQV SVISRPEQAT SEGLDHGSVF IAAPTAKQDC KTLQATCTSL
RELPLTLPDK LNEVTVPTHG FAEQARNSST FKKETDTACS NQYNPGNRIC WSEDPMRNTA
PPVVSHSSSS KQKEHPEQTG LKAVKTEHVS YAHVSDLHPR NLITNVSLPV KPEPHEVDKG
FRMDTEDFPG PERPPPVTEV TSSASVQPTQ TMKPSTTSPV EEAISLAPDT LKRIPSASSS
SCRLSSVEAN NPLVTQLLQG NLPLEKVLPQ PRLGAKLEIN RLPLPLQTTS VGKTGLERNM
VEMPSSSPNP DGKGYLAGTL APVQMRKREN HPKKRAARTV GDHAQVKCEP GKMVMEPDVK
AVPCVISPSM SQLGHNQPFK QERLNKPSMA NRIMPSPEVK QQKRLLPACS FQPSLFHVNK
NEGFHADTGT SHRQQFYQMP MAARGPLPTP ALLQNSPKTP VGCNAFAFNR HLEQKALGDV
NLPTAPHQLR LANMLSPNMP IKEGEDGGGT THTMPSKAVV HAPLPPPPPP PPPPPPPLAL
PPPPPPPPPL PPPLPTVEVP SDQKQPPVNM ETTKRLSWPQ STGICSNIKS EPLSFEESLS
SSCELGMKQV PYDQNEVKEQ LKAFALKNAD FSSYLLSEPQ KPFTQLAAQK LPVPQQQPLC
GSYPTIHFGS TNFKRAASAI EKSIGILGSG SNPATSTGLT GQNTQMPVQN FADNSNADEL
ELKCSCRLKA MIVCKGCGAF CHDDCIGPSK LCVACLVVR
