ASY3_ARATH
ID ASY3_ARATH Reviewed; 793 AA.
AC Q0WR66; O80726; Q8RY31;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Meiosis-specific protein ASY3 {ECO:0000305};
DE AltName: Full=Protein ASYNAPTIC 3 {ECO:0000303|PubMed:22319460};
DE Short=AtASY3 {ECO:0000303|PubMed:22319460};
GN Name=ASY3 {ECO:0000303|PubMed:22319460};
GN OrderedLocusNames=At2g46980 {ECO:0000312|Araport:AT2G46980};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH ASY1, AND SUBCELLULAR LOCATION.
RX PubMed=22319460; DOI=10.1371/journal.pgen.1002507;
RA Ferdous M., Higgins J.D., Osman K., Lambing C., Roitinger E., Mechtler K.,
RA Armstrong S.J., Perry R., Pradillo M., Cunado N., Franklin F.C.;
RT "Inter-homolog crossing-over and synapsis in Arabidopsis meiosis are
RT dependent on the chromosome axis protein AtASY3.";
RL PLoS Genet. 8:E1002507-E1002507(2012).
CC -!- FUNCTION: Required for normal meiosis in male and female gametophytes.
CC Acts with ASY1 at the interface between the developing chromosome axes
CC and the recombination machinery to ensure interhomolog recombination.
CC Required for synaptonemal complex formation during meiosis.
CC {ECO:0000269|PubMed:22319460}.
CC -!- SUBUNIT: Interacts with ASY1. {ECO:0000269|PubMed:22319460}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:22319460}. Nucleus
CC {ECO:0000269|PubMed:22319460}. Note=During interphase-early leptotene,
CC distributed as numerous punctuate foci throughout the chromatin. At
CC zygotene and pachytene, shows a continuous localization along
CC chromosomal axes. {ECO:0000269|PubMed:22319460}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WR66-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WR66-2; Sequence=VSP_058718, VSP_058719;
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DR EMBL; AC004411; AAC34237.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10780.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10781.1; -; Genomic_DNA.
DR EMBL; AY078934; AAL84940.1; -; mRNA.
DR EMBL; AK228456; BAF00383.1; -; mRNA.
DR PIR; T02189; T02189.
DR RefSeq; NP_182221.1; NM_130266.2. [Q0WR66-2]
DR RefSeq; NP_850466.2; NM_180135.3. [Q0WR66-1]
DR AlphaFoldDB; Q0WR66; -.
DR SMR; Q0WR66; -.
DR STRING; 3702.AT2G46980.2; -.
DR iPTMnet; Q0WR66; -.
DR PaxDb; Q0WR66; -.
DR ProteomicsDB; 246715; -. [Q0WR66-1]
DR EnsemblPlants; AT2G46980.1; AT2G46980.1; AT2G46980. [Q0WR66-2]
DR EnsemblPlants; AT2G46980.2; AT2G46980.2; AT2G46980. [Q0WR66-1]
DR GeneID; 819312; -.
DR Gramene; AT2G46980.1; AT2G46980.1; AT2G46980. [Q0WR66-2]
DR Gramene; AT2G46980.2; AT2G46980.2; AT2G46980. [Q0WR66-1]
DR KEGG; ath:AT2G46980; -.
DR Araport; AT2G46980; -.
DR TAIR; locus:2041334; AT2G46980.
DR eggNOG; ENOG502R6IY; Eukaryota.
DR InParanoid; Q0WR66; -.
DR OrthoDB; 233900at2759; -.
DR PhylomeDB; Q0WR66; -.
DR PRO; PR:Q0WR66; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q0WR66; baseline and differential.
DR GO; GO:0005694; C:chromosome; IDA:TAIR.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:TAIR.
DR GO; GO:0090173; P:regulation of synaptonemal complex assembly; IMP:UniProtKB.
DR InterPro; IPR037731; ASY3-like.
DR PANTHER; PTHR36027; PTHR36027; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Coiled coil; Meiosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..793
FT /note="Meiosis-specific protein ASY3"
FT /id="PRO_0000438698"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 305..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 682..745
FT /evidence="ECO:0000255"
FT COMPBIAS 1..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..91
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..260
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..337
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 338..357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..406
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..443
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..506
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 508..552
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..586
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 512..516
FT /note="DFLEK -> VTFIN (in isoform 2)"
FT /id="VSP_058718"
FT VAR_SEQ 517..793
FT /note="Missing (in isoform 2)"
FT /id="VSP_058719"
FT CONFLICT 262
FT /note="R -> G (in Ref. 3; AAL84940)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 793 AA; 87996 MW; 35153871CFE57CB2 CRC64;
MSDYRSFGSN YHPSSQSRKI SIGVMADSQP KRNLVPDKDD GDVIARVEKL KSATVTELQA
NKKEKSDLAA KQRNSAQVTG HVTSPWRSPR SSHRKLGTLE SVLCKQTSSL SGSKGLNKGL
NGAHQTPARE SFQNCPISSP QHSLGELNGG RNDRVMDRSP ERMEEPPSAV LQQKVASQRE
KMDKPGKETN GTTDVLRSKL WEILGKASPA NNEDVNSETP EVEKTNFKLS QDKGSNDDPL
IKPRHNSDSI ETDSESPENA TRRPVTRSLL QRRVGAKGVQ KKTKAGANLG RKCTEQVNSV
FSFEEGLRGK IGTAVNSSVM PKKQRGRRKN TVVKCRKAHS RKKDEADWSR KEASKSNTPP
RSESTETGKR SSSSDKKGSS HDLHPQSKAR KQKPDISTRE GDFHPSPEAE AAALPEMSQG
LSKNGDKHER PSNIFREKSV EPENEFQSPT FGYKAPISSP SPCCSPEASP LQPRNISPTL
DETETPIFSF GTKKTSQGTT GQASDTEKRL PDFLEKKRDY SFRRESSPEP NEDLVLSDPS
SDERDSDGSR EDSPVLGHNI SPEERETANW TNERSMLGPS SVKRNSNLKG IGRVVLSPPS
PLSKGIDKTD SFQHCSEMDE DEDEGLGRAV ALFAMALQNF ERKLKSAAEK KSSEIIASVS
EEIHLELENI KSHIITEAGK TSNLAKTKRK HAETRLQEQE EKMRMIHEKF KDDVSHHLED
FKSTIEELEA NQSELKGSIK KQRTSHQKLI AHFEGGIETK LDDATKRIDS VNKSARGKML
QLKMIVAECL RDD
