ID   PLS_STAAC               Reviewed;        1548 AA.
AC   Q5HJU7;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Putative surface protein SACOL0050;
DE   Flags: Precursor;
GN   OrderedLocusNames=SACOL0050;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}.
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DR   EMBL; CP000046; AAW38699.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5HJU7; -.
DR   SMR; Q5HJU7; -.
DR   EnsemblBacteria; AAW38699; AAW38699; SACOL0050.
DR   KEGG; sac:SACOL0050; -.
DR   HOGENOM; CLU_000977_2_1_9; -.
DR   OMA; EEVPQGH; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   InterPro; IPR011098; G5_dom.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR031477; SasG_E.
DR   InterPro; IPR005877; YSIRK_signal_dom.
DR   Pfam; PF07501; G5; 4.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF17041; SasG_E; 4.
DR   Pfam; PF04650; YSIRK_signal; 1.
DR   SMART; SM01208; G5; 4.
DR   TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR   PROSITE; PS51109; G5; 4.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   3: Inferred from homology;
KW   Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT   SIGNAL          1..48
FT                   /evidence="ECO:0000255"
FT   CHAIN           49..1512
FT                   /note="Putative surface protein SACOL0050"
FT                   /id="PRO_0000045208"
FT   PROPEP          1513..1548
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000045209"
FT   DOMAIN          667..750
FT                   /note="G5 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          796..878
FT                   /note="G5 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          924..1006
FT                   /note="G5 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          1052..1135
FT                   /note="G5 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   REGION          49..318
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          702..732
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          766..1522
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           1509..1513
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   COMPBIAS        49..86
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        108..286
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        293..317
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        795..833
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        834..849
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        923..961
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        962..977
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1051..1072
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1092..1106
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1156..1188
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1189..1489
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1490..1509
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1512
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ   SEQUENCE   1548 AA;  164848 MW;  021480D35BEB899C CRC64;
     MNKNSKKKLD FLPNKLNKYS IRRFTVGTAS ILVGATLIFG VANDQAEAAE NNTTQKQDDS
     SDASKVKGNV QTIEQSSANS NESDIPEQVD VTKDTTEQAS TEEKANTTEQ ASTEEKADTT
     EQATTEEAPK AEGTDKVETE EAPKAEETDK ATEEAPKTEE TDKATTEEAP KAEETDKATE
     EAPKTEETDK ATTEEAPAAE ETSKAATEEA PKAEETSKAA TEEAPKAEET EKTATEEAPK
     TEETDKVETE EAPKAEETSK AATEKAPKAE ETNKVETEEA PAAEETNKAA TEETPAVEDT
     NAKSNSNAQP SETERTQVVD TVAKDLYKKS EVTEAEKAEI EKVLPKDISN LSNEEIKKIA
     LSEVLKETAN KENAQPRATF RSVSSNARTT NVNYSATALR AAAQDTVTKK GTGNFTAHGD
     IIHKTYKEEF PNEGTLTAFN TNFNPNTGTK GALEYNDKID FNKDFTITVP VANNNQGNTT
     GADGWGFMFT QGNGQDFLNQ GGILRDKGMA NASGFKIDTA YNNVNGKVDK LDADKTNNLS
     QIGAAKVGYG TFVKNGADGV TNQVGQNALN TKDKPVNKII YADNTTNHLD GQFHGQRLND
     VVLNYDAATS TITATYAGKT WKATTDDLGI DKSQKYNFLI TSSHMQNRYS NGIMRTNLEG
     VTITTPQADL IDDVEVTKQP IPHKTIREFD PTLEPGSPDV IVQKGEDGEK TTTTPTKVDP
     DTGDVVERGE PTTEVTKNPV DEIVHFAPEE VPQGHKDEFD PNLPIDGTEE VPGKPGIKNP
     ETGEVVTPPV DDVTKHGPKA GEPEVTKEEI PFEKKREFNP DLKPGEEKVT QEGQTGEKTT
     TTPTTINPLT GEKVGEGEPT TEVTKEPVDE ITQFGGEEVP QGHKDEFDPN LPIDGTEEVP
     GKPGIKNPET GEVVTPPVDD VTKHGPKAGE PEVTKEEIPF EKKREFNPDL KPGEEKVTQE
     GQTGEKTTTT PTTINPLTGE KVGEGEPTTE VTKEPVDEIT QFGGEEVPQG HKDEFDPNLP
     IDGTEEVPGK PGIKNPETGE VVTPPVDDVT KHGPKAGEPE VTKEEIPYET KRVLDPTMEP
     GSPDKVAQKG ENGEKTTTTP TTINPLTGEK VGEGEPTTEV TKEPIDEIVN YAPEIIPHGT
     REEIDPNLPE GETKVIPGKD GLKDPETGEI IEEPQDEVII HGAKDDSDAD SDSDADSDSD
     ADSDSDADSD SDADSDSDSD SDSDSDSDSD ADSDSDADSD SDSDSDSDAD SDSDADSDSD
     ADSDSDADSD SDSDSDSDAD SDSDSDSDSD ADSDSDADSD SDADSDSDAD SDSDSDSDSD
     ADSDSDSDSD SDSDADSDSD SDSDADSDSD ADSDSDADSD SDADSDSDSD SDSDSDSDSD
     SDSDRDSDAD SDSDADSDSD SDSDSDADSD SDSDSDSDAD SDSDADSDSD SDADSDSDSD
     SDSDADSDSD ADSDSDADSD SDADSDSDSD SDSDSDSDSD ADSDSDADSD SDADRDHNDK
     TDKPNNKELP DTGNDAQNNG TLFGSLFAAL GGLFLVGRRR KNKNNEEK