ID   PLS_STAAM               Reviewed;        1114 AA.
AC   Q931E9; Q99RD7;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2004, sequence version 2.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Putative surface protein SAV2496/SAV2497;
DE   Flags: Precursor;
GN   OrderedLocusNames=SAV2496/SAV2497;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB58658.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB58659.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
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DR   EMBL; BA000017; BAB58658.2; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000017; BAB58659.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; Q931E9; -.
DR   SMR; Q931E9; -.
DR   PaxDb; Q931E9; -.
DR   EnsemblBacteria; BAB58658; BAB58658; SAV2496.
DR   EnsemblBacteria; BAB58659; BAB58659; SAV2497.
DR   KEGG; sav:SAV2496; -.
DR   KEGG; sav:SAV2497; -.
DR   HOGENOM; CLU_115201_0_0_9; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   InterPro; IPR011098; G5_dom.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR031477; SasG_E.
DR   InterPro; IPR005877; YSIRK_signal_dom.
DR   Pfam; PF07501; G5; 5.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF17041; SasG_E; 4.
DR   Pfam; PF04650; YSIRK_signal; 1.
DR   SMART; SM01208; G5; 5.
DR   TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR   PROSITE; PS51109; G5; 5.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   3: Inferred from homology;
KW   Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT   SIGNAL          1..50
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..1085
FT                   /note="Putative surface protein SAV2496/SAV2497"
FT                   /id="PRO_0000005633"
FT   PROPEP          1086..1114
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000005634"
FT   DOMAIN          419..501
FT                   /note="G5 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          547..628
FT                   /note="G5 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          674..756
FT                   /note="G5 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          802..884
FT                   /note="G5 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   DOMAIN          930..1012
FT                   /note="G5 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   REGION          76..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          440..473
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          496..1088
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           1082..1086
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   COMPBIAS        92..116
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        440..458
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        502..526
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        552..586
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        629..653
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        679..713
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        757..781
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        807..841
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        861..890
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        918..933
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        943..967
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        968..982
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1000..1024
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1050..1085
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1085
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ   SEQUENCE   1114 AA;  122209 MW;  41927F43E2133F44 CRC64;
     MRDKKGPVNK RVDFLSNKLN KYSIRKFTVG TASILIGSLM YLGTQQEAEA AENNIENPTT
     LKDNVQSKEV KIEEVTNKDT APQGVEAKSE VTSNKDTIEH EASVKAEDIS KKEDTPKEVA
     NVAEVQPKSS VTHNAEAPKV RKARSVDEGS FDITRDSKNV VESTPITIQG KEHFEGYGSV
     DIQKNPTDLG VSEVTRFNVG NESNGLIGAL QLKNKIDFSK DFNFKVRVAN NHQSNTTGAD
     GWGFLFSKGN AEEYLTNGGI LGDKGLVNSG GFKIDTGYIY TSSMDKTEKQ AGQGYRGYGA
     FVKNDSSGNS QMVGENIDKS KTNFLNYADN STNTSDGKFH GQRLNDVILT YVASTGKMRA
     EYAGKTWETS ITDLGLSKNQ AYNFLITSSQ RWGLNQGINA NGWMRTDLKG SEFTFTPRSA
     KNNNRIRKKV EEIPFKKERK FNPDLAPGTE KVTREGQKGE KTITTPTLKN PLTGEIISKG
     ESKEEITKDP INELTEYGPE TIAPGHRDEF DPKLPTGEKE EVPGKPGIKN PETGDVVRPP
     VDSVTKYGPV KGDSIVEKEE IPFEKERKFN PDLAPGTEKV TREGQKGEKT TTPTLKNPLT
     GEIISKGESK EEITKDPINE LTEYGPETIA PGHRDEFDPK LPTGEKEEVP GKPGIKNPET
     GDVVRPPVDS VTKYGPVKGD SIVEKEEIPF KKERKFNPDL APGTEKVTRE GQKGEKTITT
     PTLKNPLTGE IISKGESKEE ITKDPINELT EYGPETITPG HRDEFDPKLP TGEKEEVPGK
     PGIKNPETGD VVRPPVDSVT KYGPVKGDSI VEKEEIPFEK ERKFNPDLAP GTEKVTREGQ
     KGEKTITTPT LKNPLTGEII SKGESKEEIT KDPVNELTEF GGEKIPQGHK DIFDPNLPTD
     QTEKVPGKPG IKNPDTGKVI EEPVDDVIKH GPKTGTPETK TVEIPFETKR EFNPKLQPGE
     ERVKQEGQPG SKTITTPITV NPLTGEKVGE GQPTEEITKQ PVDKIVEFGG EKPKDPKGPE
     NPEKPSRPTH PSGPVNPNNP GLSKDRAKPN GPVHSMDKND KVKKSKIAKE SVANQEKKRA
     ELPKTGLEST QKGLIFSSII GIAGLMLLAR RRKN