PLS_STAAN
ID PLS_STAAN Reviewed; 1370 AA.
AC P61598; Q99RD7;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Putative surface protein SA2285;
DE Flags: Precursor;
GN OrderedLocusNames=SA2285;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N315;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=N315;
RA Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT aureus strain N315.";
RL Submitted (OCT-2007) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB43588.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; BA000018; BAB43588.1; ALT_FRAME; Genomic_DNA.
DR PIR; B90053; B90053.
DR AlphaFoldDB; P61598; -.
DR SMR; P61598; -.
DR EnsemblBacteria; BAB43588; BAB43588; BAB43588.
DR KEGG; sau:SA2285; -.
DR HOGENOM; CLU_000977_2_0_9; -.
DR Proteomes; UP000000751; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR InterPro; IPR011098; G5_dom.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR031477; SasG_E.
DR InterPro; IPR005877; YSIRK_signal_dom.
DR Pfam; PF07501; G5; 7.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF17041; SasG_E; 6.
DR Pfam; PF04650; YSIRK_signal; 1.
DR SMART; SM01208; G5; 7.
DR TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR PROSITE; PS51109; G5; 7.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 1: Evidence at protein level;
KW Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT SIGNAL 1..50
FT /evidence="ECO:0000255"
FT CHAIN 51..1341
FT /note="Putative surface protein SA2285"
FT /id="PRO_0000005635"
FT PROPEP 1342..1370
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000005636"
FT DOMAIN 418..500
FT /note="G5 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 546..628
FT /note="G5 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 674..756
FT /note="G5 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 802..884
FT /note="G5 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 930..1012
FT /note="G5 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 1058..1140
FT /note="G5 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 1186..1268
FT /note="G5 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT REGION 77..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 439..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..1344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1338..1342
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 92..116
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..457
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..525
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..585
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 629..653
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 679..713
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 757..781
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..841
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 885..909
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 935..969
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1013..1037
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1063..1097
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1117..1146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1174..1189
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1199..1223
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1224..1238
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1256..1280
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1306..1341
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1341
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ SEQUENCE 1370 AA; 150227 MW; 9D3EF22A2C511066 CRC64;
MRDKKGPVNK RVDFLSNKLN KYSIRKFTVG TASILIGSLM YLGTQQEAEA AENNIENPTT
LKDNVQSKEV KIEEVTNKDT APQGVEAKSE VTSNKDTIEH EASVKAEDIS KKEDTPKEVA
NVAEVQPKSS VTHNAEAPKV RKARSVDEGS FDITRDSKNV VESTPITIQG KEHFEGYGSV
DIQKNPTDLG VSEVTRFNVG NESNGLIGAL QLKNKIDFSK DFNFKVRVAN NHQSNTTGAD
GWGFLFSKGN AEEYLTNGGI LGDKGLVNSG GFKIDTGYIY TSSMDKTEKQ AGQGYRGYGA
FVKNDSSGNS QMVGENIDKS KTNFLNYADN STNTSDGKFH GQRLNDVILT YVASTGKMRA
EYAGKTWETS ITDLGLSKNQ AYNFLITSSQ RWGLNQGINA NGWMRTDLKG SEFTFTPSAK
NNNRIRKKVE EIPFKKERKF NPDLAPGTEK VTREGQKGEK TITTPTLKNP LTGEIISKGE
SKEEITKDPI NELTEYGPET IAPGHRDEFD PKLPTGEKEE VPGKPGIKNP ETGDVVRPPV
DSVTKYGPVK GDSIVEKEEI PFEKERKFNP DLAPGTEKVT REGQKGEKTI TTPTLKNPLT
GEIISKGESK EEITKDPINE LTEYGPETIA PGHRDEFDPK LPTGEKEEVP GKPGIKNPET
GDVVRPPVDS VTKYGPVKGD SIVEKEEIPF EKERKFNPDL APGTEKVTRE GQKGEKTITT
PTLKNPLTGE IISKGESKEE ITKDPINELT EYGPETIAPG HRDEFDPKLP TGEKEEVPGK
PGIKNPETGD VVRPPVDSVT KYGPVKGDSI VEKEEIPFEK ERKFNPDLAP GTEKVTREGQ
KGEKTITTPT LKNPLTGEII SKGESKEEIT KDPINELTEY GPETIAPGHR DEFDPKLPTG
EKEEVPGKPG IKNPETGDVV RPPVDSVTKY GPVKGDSIVE KEEIPFKKER KFNPDLAPGT
EKVTREGQKG EKTITTPTLK NPLTGEIISK GESKEEITKD PINELTEYGP ETITPGHRDE
FDPKLPTGEK EEVPGKPGIK NPETGDVVRP PVDSVTKYGP VKGDSIVEKE EIPFEKERKF
NPDLAPGTEK VTREGQKGEK TITTPTLKNP LTGEIISKGE SKEEITKDPV NELTEFGGEK
IPQGHKDIFD PNLPTDQTEK VPGKPGIKNP DTGKVIEEPV DDVIKHGPKT GTPETKTVEI
PFETKREFNP KLQPGEERVK QEGQPGSKTI TTPITVNPLT GEKVGEGQPT EEITKQPVDK
IVEFGGEKPK DPKGPENPEK PSRPTHPSGP VNPNNPGLSK DRAKPNGPVH SMDKNDKVKK
SKIAKESVAN QEKKRAELPK TGLESTQKGL IFSSIIGIAG LMLLARRRKN
