PLS_STAAU
ID PLS_STAAU Reviewed; 1637 AA.
AC P80544; Q9ZF62;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Surface protein;
DE AltName: Full=230 kDa cell-wall protein;
DE AltName: Full=Plasmin-sensitive surface protein;
DE Flags: Precursor;
GN Name=pls;
OS Staphylococcus aureus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate 1061;
RX PubMed=11292719; DOI=10.1128/iai.69.5.3013-3020.2001;
RA Savolainen K., Paulin L., Westerlund-Wikstrom B., Foster T.J.,
RA Korhonen T.K., Kuusela P.;
RT "Expression of pls, a gene closely associated with the mecA gene of
RT methicillin-resistant Staphylococcus aureus, prevents bacterial adhesion in
RT vitro.";
RL Infect. Immun. 69:3013-3020(2001).
RN [2]
RP PROTEIN SEQUENCE OF 624-628; 676-682; 938-948; 1156-1168; 1176-1185;
RP 1199-1205 AND 1217-1224.
RC STRAIN=Isolate 1061;
RX PubMed=8665912; DOI=10.1111/j.1432-1033.1996.00904.x;
RA Hilden P., Savolainen K., Tyynelae J., Vuento M., Kuusela P.;
RT "Purification and characterisation of a plasmin-sensitive surface protein
RT of Staphylococcus aureus.";
RL Eur. J. Biochem. 236:904-910(1996).
CC -!- FUNCTION: Could have a role in preventing adhesion at some stages
CC during an infection.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}.
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DR EMBL; AF115379; AAD09131.1; -; Genomic_DNA.
DR AlphaFoldDB; P80544; -.
DR SMR; P80544; -.
DR PRIDE; P80544; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR InterPro; IPR011098; G5_dom.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR031477; SasG_E.
DR InterPro; IPR005877; YSIRK_signal_dom.
DR Pfam; PF07501; G5; 5.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF17041; SasG_E; 5.
DR Pfam; PF04650; YSIRK_signal; 1.
DR SMART; SM01208; G5; 5.
DR TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR PROSITE; PS51109; G5; 5.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 1: Evidence at protein level;
KW Cell wall; Direct protein sequencing; Peptidoglycan-anchor; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..48
FT /evidence="ECO:0000255"
FT CHAIN 49..1601
FT /note="Surface protein"
FT /id="PRO_0000005639"
FT PROPEP 1602..1637
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000005640"
FT DOMAIN 654..737
FT /note="G5 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 783..865
FT /note="G5 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 911..993
FT /note="G5 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 1039..1121
FT /note="G5 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 1167..1250
FT /note="G5 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT REPEAT 1301..1302
FT /note="1"
FT REPEAT 1303..1304
FT /note="2"
FT REPEAT 1305..1306
FT /note="3"
FT REPEAT 1307..1308
FT /note="4"
FT REPEAT 1309..1310
FT /note="5"
FT REPEAT 1311..1312
FT /note="6"
FT REPEAT 1313..1314
FT /note="7"
FT REPEAT 1315..1316
FT /note="8"
FT REPEAT 1317..1318
FT /note="9"
FT REPEAT 1319..1320
FT /note="10"
FT REPEAT 1321..1322
FT /note="11"
FT REPEAT 1323..1324
FT /note="12"
FT REPEAT 1325..1326
FT /note="13"
FT REPEAT 1327..1328
FT /note="14"
FT REPEAT 1329..1330
FT /note="15"
FT REPEAT 1331..1332
FT /note="16"
FT REPEAT 1333..1334
FT /note="17"
FT REPEAT 1335..1336
FT /note="18"
FT REPEAT 1337..1338
FT /note="19"
FT REPEAT 1339..1340
FT /note="20"
FT REPEAT 1341..1342
FT /note="21"
FT REPEAT 1343..1344
FT /note="22"
FT REPEAT 1345..1346
FT /note="23"
FT REPEAT 1347..1348
FT /note="24"
FT REPEAT 1349..1350
FT /note="25"
FT REPEAT 1351..1352
FT /note="26"
FT REPEAT 1353..1354
FT /note="27"
FT REPEAT 1355..1356
FT /note="28"
FT REPEAT 1357..1358
FT /note="29"
FT REPEAT 1359..1360
FT /note="30"
FT REPEAT 1361..1362
FT /note="31"
FT REPEAT 1363..1364
FT /note="32"
FT REPEAT 1365..1366
FT /note="33"
FT REPEAT 1367..1368
FT /note="34"
FT REPEAT 1369..1370
FT /note="35"
FT REPEAT 1371..1372
FT /note="36"
FT REPEAT 1373..1374
FT /note="37"
FT REPEAT 1375..1376
FT /note="38"
FT REPEAT 1377..1378
FT /note="39"
FT REPEAT 1379..1380
FT /note="40"
FT REPEAT 1381..1382
FT /note="41"
FT REPEAT 1383..1384
FT /note="42"
FT REPEAT 1385..1386
FT /note="43"
FT REPEAT 1387..1388
FT /note="44"
FT REPEAT 1389..1390
FT /note="45"
FT REPEAT 1391..1392
FT /note="46"
FT REPEAT 1393..1394
FT /note="47"
FT REPEAT 1395..1396
FT /note="48"
FT REPEAT 1397..1398
FT /note="49"
FT REPEAT 1399..1400
FT /note="50"
FT REPEAT 1401..1402
FT /note="51"
FT REPEAT 1403..1404
FT /note="52"
FT REPEAT 1405..1406
FT /note="53"
FT REPEAT 1407..1408
FT /note="54"
FT REPEAT 1409..1410
FT /note="55"
FT REPEAT 1411..1412
FT /note="56"
FT REPEAT 1413..1414
FT /note="57"
FT REPEAT 1415..1416
FT /note="58"
FT REPEAT 1417..1418
FT /note="59"
FT REPEAT 1419..1420
FT /note="60"
FT REPEAT 1421..1422
FT /note="61"
FT REPEAT 1423..1424
FT /note="62"
FT REPEAT 1425..1426
FT /note="63"
FT REPEAT 1427..1428
FT /note="64"
FT REPEAT 1429..1430
FT /note="65"
FT REPEAT 1431..1432
FT /note="66"
FT REPEAT 1433..1434
FT /note="67"
FT REPEAT 1435..1436
FT /note="68"
FT REPEAT 1437..1438
FT /note="69"
FT REPEAT 1439..1440
FT /note="70"
FT REPEAT 1441..1442
FT /note="71"
FT REPEAT 1443..1444
FT /note="72"
FT REPEAT 1445..1446
FT /note="73"
FT REPEAT 1447..1448
FT /note="74"
FT REPEAT 1449..1450
FT /note="75"
FT REPEAT 1451..1452
FT /note="76"
FT REPEAT 1453..1454
FT /note="77"
FT REPEAT 1455..1456
FT /note="78"
FT REPEAT 1457..1458
FT /note="79"
FT REPEAT 1459..1460
FT /note="80"
FT REPEAT 1461..1462
FT /note="81"
FT REPEAT 1463..1464
FT /note="82"
FT REPEAT 1465..1466
FT /note="83"
FT REPEAT 1467..1468
FT /note="84"
FT REPEAT 1469..1470
FT /note="85"
FT REPEAT 1471..1472
FT /note="86"
FT REPEAT 1473..1474
FT /note="87"
FT REPEAT 1475..1476
FT /note="88"
FT REPEAT 1477..1478
FT /note="89"
FT REPEAT 1479..1480
FT /note="90"
FT REPEAT 1481..1482
FT /note="91"
FT REPEAT 1483..1484
FT /note="92"
FT REPEAT 1485..1486
FT /note="93"
FT REPEAT 1487..1488
FT /note="94"
FT REPEAT 1489..1490
FT /note="95"
FT REPEAT 1491..1492
FT /note="96"
FT REPEAT 1493..1494
FT /note="97"
FT REPEAT 1495..1496
FT /note="98"
FT REPEAT 1497..1498
FT /note="99"
FT REPEAT 1499..1500
FT /note="100"
FT REPEAT 1501..1502
FT /note="101"
FT REPEAT 1503..1504
FT /note="102"
FT REPEAT 1505..1506
FT /note="103"
FT REPEAT 1507..1508
FT /note="104"
FT REPEAT 1509..1510
FT /note="105"
FT REPEAT 1511..1512
FT /note="106"
FT REPEAT 1513..1514
FT /note="107"
FT REPEAT 1515..1516
FT /note="108"
FT REPEAT 1517..1518
FT /note="109"
FT REPEAT 1519..1520
FT /note="110"
FT REPEAT 1521..1522
FT /note="111"
FT REPEAT 1523..1524
FT /note="112"
FT REPEAT 1525..1526
FT /note="113"
FT REPEAT 1527..1528
FT /note="114"
FT REPEAT 1529..1530
FT /note="115"
FT REPEAT 1531..1532
FT /note="116"
FT REPEAT 1533..1534
FT /note="117"
FT REPEAT 1535..1536
FT /note="118"
FT REPEAT 1537..1538
FT /note="119"
FT REPEAT 1539..1540
FT /note="120"
FT REPEAT 1541..1542
FT /note="121"
FT REPEAT 1543..1544
FT /note="122"
FT REPEAT 1545..1546
FT /note="123"
FT REPEAT 1547..1548
FT /note="124"
FT REPEAT 1549..1550
FT /note="125"
FT REPEAT 1551..1552
FT /note="126"
FT REPEAT 1553..1554
FT /note="127"
FT REPEAT 1555..1556
FT /note="128"
FT REPEAT 1557..1558
FT /note="129"
FT REPEAT 1559..1560
FT /note="130"
FT REPEAT 1561..1562
FT /note="131"
FT REPEAT 1563..1564
FT /note="132"
FT REPEAT 1565..1566
FT /note="133"
FT REPEAT 1567..1568
FT /note="134"
FT REPEAT 1569..1570
FT /note="135"
FT REPEAT 1571..1572
FT /note="136"
FT REPEAT 1573..1574
FT /note="137"
FT REPEAT 1575..1576
FT /note="138"
FT REPEAT 1577..1578
FT /note="139"
FT REPEAT 1579..1580
FT /note="140"
FT REPEAT 1581..1582
FT /note="141"
FT REGION 49..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 689..719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 739..1611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1301..1582
FT /note="141 X 2 AA tandem repeats of D-[SAG]"
FT MOTIF 1598..1602
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 49..86
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 280..304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 782..820
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 821..836
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 910..948
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 949..964
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1038..1076
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1077..1092
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1166..1187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1207..1221
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1271..1303
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1304..1578
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1579..1598
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1601
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ SEQUENCE 1637 AA; 174575 MW; 75BE9ADB469BD309 CRC64;
MNKNSKKKLD FLPNKLNKYS IRRFTVGTAS ILVGATLIFG VANDQAEAAE NNTTQKQDDS
SDASKVKGNV QTIEQSSANS NESDIPEQVD VTKDTTEQAS TEEKANTTEQ ASTEEKADTT
EQATTEEAPK AEGTDKVETE EAPKAEETDK ATTEEAPKAE ETDKATEEAP KTEETDKATT
EEAPAAEETS KAATEEAPKA EETSKAATEE APKAEETEKT ATEEAPKTEE TDKVETEEAP
KAEETSKAAT EKAPKAEETN KVETEEAPAA EETNKAATEE TPAVEDTNAK SNSNAQPSET
ERTQVVDTVA KDLYKKSEVT EAEKAEIEKV LPKDISNLSN EEIKKIALSE VLKETANKEN
AQPRATFRSV SSNARTTNVN YSATALRAAA QDTVTKKGTG NFTAHGDIIH KTYKEEFPNE
GTLTAFNTNF NPNTGTKGAL EYNDKIDFNK DFTITVPVAN NNQGNTTGAD GWGFMFTQGN
GQDFLNQGGI LRDKGMANAS GFKIDTAYNN VNGKVDKLDA DKTNNLSQIG AAKVGYGTFV
KNGADGVTNQ VGQNALNTKD KPVNKIIYAD NTTNHLDGQF HGQRLNDVVL NYDAATSTIT
ATYAGKTWKA TTDDLGIDKS QKYNFLITSS HMQNRYSNGI MRTNLEGVTI TTPQADLIDD
VEVTKQPIPH KTIREFDPTL EPGSPDVIVQ KGEDGEKTTT TPTKVDPDTG DVVERGEPTT
EVTKNPVDEI VHFTPEEVPQ GHKDEFDPNL PIDGTEEVPG KPGIKNPETG EVVTPPVDDV
TKHGPKAGEP EVTKEEIPFE KKREFNPDLK PGEEKVTQEG QTGEKTTTTP TTINPLTGEK
VGEGEPTTEV TKEPVDEITQ FGGEEVPQGH KDEFDPNLPI DGTEEVPGKP GIKNPETGEV
VTPPVDDVTK HGPKAGEPEV TKEEIPFEKK REFNPDLKPG EEKVTQEGQT GEKTTTTPTT
INPLTGEKVG EGEPTTEVTK EPVDEITQFG GEEVPQGHKD EFDPNLPIDG TEEVPGKPGI
KNPETGEVVT PPVDDVTKHG PKAGEPEVTK EEIPFEKKRE FNPDLKPGEE KVTQEGQTGE
KTTTTPTTIN PLTGEKVGEG EPTTEVTKEP VDEITQFGGE EVPQGHKDEF DPNLPIDGTE
EVPGKPGIKN PETGEVVTPP VDDVTKHGPK AGEPEVTKEE IPYETKRVLD PTMEPGSPDK
VAQKGENGEK TTTTPTTINP LTGEKVGEGE PTTEVTKEPI DEIVNYAPEI IPHGTREEID
PNLPEGETKV IPGKDGLKDP ETGEIIEEPQ DEVIIHGAKD DSDADSDSDA DSDSDADSDS
DADSDSDADS DSDSDSDSDS DSDSDADSDS DSDSDSDADS DSDADSDSDA DSDSDSDADS
DSDSDADSDS DSDSDSDADS DSDSDSDSDA DSDSDADSDS DSDSDSDADS DSDSDSDSDA
DSDSDADSDS DADSDSDADS DSDSDSDSDA DSDSDADSDS DADSDSDADS DSDSDSDSDA
DSDSDSDSDS DSDADSDSDA DSDSDSDADS DSDADSDSDA DGDSDADSDS DADSDSDSDS
DSDSDSDSDA DSDSDSDSDS DADRDHNDKT DKPNNKELPD TGNDAQNNGT LFGSLFAALG
GLFLVGRRRK NKNNEEK
