PLT5_ARATH
ID PLT5_ARATH Reviewed; 539 AA.
AC Q8VZ80; Q9LS92;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Polyol transporter 5;
DE AltName: Full=Protein POLYOL TRANSPORTER 5;
DE Short=AtPLT5;
DE AltName: Full=Sugar-proton symporter PLT5;
GN Name=PLT5; OrderedLocusNames=At3g18830; ORFNames=MVE11.10, MVE11.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 249-527.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=15525644; DOI=10.1074/jbc.m410831200;
RA Reinders A., Panshyshyn J.A., Ward J.M.;
RT "Analysis of transport activity of Arabidopsis sugar alcohol permease
RT homolog AtPLT5.";
RL J. Biol. Chem. 280:1594-1602(2005).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=15598803; DOI=10.1105/tpc.104.026641;
RA Klepek Y.-S., Geiger D., Stadler R., Klebl F., Landouar-Arsivaud L.,
RA Lemoine R., Hedrich R., Sauer N.;
RT "Arabidopsis POLYOL TRANSPORTER5, a new member of the monosaccharide
RT transporter-like superfamily, mediates H+-symport of numerous substrates,
RT including myo-inositol, glycerol, and ribose.";
RL Plant Cell 17:204-218(2005).
RN [6]
RP GENE FAMILY.
RX PubMed=16923188; DOI=10.1186/1471-2148-6-64;
RA Johnson D.A., Hill J.P., Thomas M.A.;
RT "The monosaccharide transporter gene family in land plants is ancient and
RT shows differential subfamily expression and expansion across lineages.";
RL BMC Evol. Biol. 6:64-64(2006).
CC -!- FUNCTION: Plasma membrane broad-spectrum sugar-proton symporter.
CC Mediates the uptake of linear polyols such as sorbitol, xylitol,
CC erythritol or glycerol. Can transport the cyclic polyol myo-inositol
CC and different hexoses, pentoses (including ribose), tetroses and sugar
CC alcohols. {ECO:0000269|PubMed:15525644, ECO:0000269|PubMed:15598803}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.5 mM for sorbitol {ECO:0000269|PubMed:15525644,
CC ECO:0000269|PubMed:15598803};
CC KM=1.5 mM for glucose {ECO:0000269|PubMed:15525644,
CC ECO:0000269|PubMed:15598803};
CC KM=3.5 mM for myo-inositol {ECO:0000269|PubMed:15525644,
CC ECO:0000269|PubMed:15598803};
CC KM=23.4 mM for glycerol {ECO:0000269|PubMed:15525644,
CC ECO:0000269|PubMed:15598803};
CC Note=Determined at -60 mV (membrane potential) and an extracellular
CC pH of 5.5.;
CC pH dependence:
CC Optimum extracellular pH is 4.5 for sorbitol as substrate.
CC {ECO:0000269|PubMed:15525644, ECO:0000269|PubMed:15598803};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15525644,
CC ECO:0000269|PubMed:15598803}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15525644, ECO:0000269|PubMed:15598803}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots. Expressed in vascular
CC tissue of leaves, sepals and siliques. {ECO:0000269|PubMed:15525644,
CC ECO:0000269|PubMed:15598803}.
CC -!- INDUCTION: By wounding and insect feeding.
CC {ECO:0000269|PubMed:15525644}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL38359.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB026654; BAB01812.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76153.1; -; Genomic_DNA.
DR EMBL; AY065183; AAL38359.1; ALT_FRAME; mRNA.
DR EMBL; AY081618; AAM10180.1; -; mRNA.
DR RefSeq; NP_188513.1; NM_112769.3.
DR AlphaFoldDB; Q8VZ80; -.
DR SMR; Q8VZ80; -.
DR BioGRID; 6749; 2.
DR STRING; 3702.AT3G18830.1; -.
DR TCDB; 2.A.1.1.34; the major facilitator superfamily (mfs).
DR iPTMnet; Q8VZ80; -.
DR PaxDb; Q8VZ80; -.
DR PRIDE; Q8VZ80; -.
DR ProteomicsDB; 234924; -.
DR EnsemblPlants; AT3G18830.1; AT3G18830.1; AT3G18830.
DR GeneID; 821416; -.
DR Gramene; AT3G18830.1; AT3G18830.1; AT3G18830.
DR KEGG; ath:AT3G18830; -.
DR Araport; AT3G18830; -.
DR TAIR; locus:2094034; AT3G18830.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_001265_30_5_1; -.
DR InParanoid; Q8VZ80; -.
DR OMA; WNCILAF; -.
DR OrthoDB; 326501at2759; -.
DR PhylomeDB; Q8VZ80; -.
DR PRO; PR:Q8VZ80; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8VZ80; baseline and differential.
DR Genevisible; Q8VZ80; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IDA:TAIR.
DR GO; GO:0015591; F:D-ribose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015148; F:D-xylose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005354; F:galactose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015168; F:glycerol transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015575; F:mannitol transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015145; F:monosaccharide transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005365; F:myo-inositol transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015576; F:sorbitol transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0010311; P:lateral root formation; IMP:CACAO.
DR CDD; cd17437; MFS_PLT; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044776; PLT1-6.
DR InterPro; IPR045262; STP/PLT_plant.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR PANTHER; PTHR23500; PTHR23500; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..539
FT /note="Polyol transporter 5"
FT /id="PRO_0000259873"
FT TRANSMEM 37..57
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..539
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..23
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 527
FT /note="S -> L (in Ref. 3; AAL38359/AAM10180)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 539 AA; 58103 MW; 36B389284E5563A0 CRC64;
MTGATPENRT APSPPPVKHV PESVLPAKPP KRNNYAFACA ILASMTSILL GYDIGVMSGA
MIYIKRDLKI NDLQIGILAG SLNIYSLIGS CAAGRTSDWI GRRYTIVLAG AIFFAGAILM
GLSPNYAFLM FGRFIAGIGV GYALMIAPVY TAEVSPASSR GFLNSFPEVF INAGIMLGYV
SNLAFSNLPL KVGWRLMLGI GAVPSVILAI GVLAMPESPR WLVMQGRLGD AKRVLDKTSD
SPTEATLRLE DIKHAAGIPA DCHDDVVQVS RRNSHGEGVW RELLIRPTPA VRRVMIAAIG
IHFFQQASGI DAVVLFSPRI FKTAGLKTDH QQLLATVAVG VVKTSFILVA TFLLDRIGRR
PLLLTSVGGM VLSLAALGTS LTIIDQSEKK VMWAVVVAIA TVMTYVATFS IGAGPITWVY
SSEIFPLRLR SQGSSMGVVV NRVTSGVISI SFLPMSKAMT TGGAFYLFGG IATVAWVFFY
TFLPETQGRM LEDMDELFSG FRWRDSKSKP KGNPEKTVPN PEVEIGSNKQ WKEGDTQSS
