PLTF_PSEF5
ID PLTF_PSEF5 Reviewed; 498 AA.
AC Q4KCY5;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=L-proline--[L-prolyl-carrier protein] ligase {ECO:0000305};
DE EC=6.2.1.53 {ECO:0000269|PubMed:11880032};
DE AltName: Full=L-prolyl-AMP ligase {ECO:0000303|PubMed:11880032};
GN Name=pltF {ECO:0000312|EMBL:AAY92064.1};
GN OrderedLocusNames=PFL_2792 {ECO:0000312|EMBL:AAY92064.1};
OS Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=220664;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX PubMed=15980861; DOI=10.1038/nbt1110;
RA Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA Thomashow L.S., Loper J.E.;
RT "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT Pf-5.";
RL Nat. Biotechnol. 23:873-878(2005).
RN [2]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX PubMed=11880032; DOI=10.1016/s1074-5521(02)00100-x;
RA Thomas M.G., Burkart M.D., Walsh C.T.;
RT "Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during
RT undecylprodigiosin and pyoluteorin biosynthesis.";
RL Chem. Biol. 9:171-184(2002).
CC -!- FUNCTION: Involved in the biosynthesis of pyoluteorin. Catalyzes the
CC conversion of L-proline to L-prolyl-AMP and the transfer of the L-
CC prolyl group to acyl carrier protein PltL.
CC {ECO:0000269|PubMed:11880032}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + holo-[peptidyl-carrier protein] + L-proline = AMP +
CC diphosphate + L-prolyl-[peptidyl-carrier protein];
CC Xref=Rhea:RHEA:11656, Rhea:RHEA-COMP:11480, Rhea:RHEA-COMP:14109,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039,
CC ChEBI:CHEBI:64479, ChEBI:CHEBI:138622, ChEBI:CHEBI:456215;
CC EC=6.2.1.53; Evidence={ECO:0000269|PubMed:11880032};
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; CP000076; AAY92064.1; -; Genomic_DNA.
DR RefSeq; WP_011061084.1; NC_004129.6.
DR PDB; 6O6E; X-ray; 2.14 A; B=1-498.
DR PDBsum; 6O6E; -.
DR AlphaFoldDB; Q4KCY5; -.
DR SMR; Q4KCY5; -.
DR STRING; 220664.PFL_2792; -.
DR DNASU; 3478039; -.
DR EnsemblBacteria; AAY92064; AAY92064; PFL_2792.
DR KEGG; pfl:PFL_2792; -.
DR PATRIC; fig|220664.5.peg.2848; -.
DR eggNOG; COG1020; Bacteria.
DR HOGENOM; CLU_000022_2_12_6; -.
DR OMA; RTLYPIG; -.
DR OrthoDB; 377638at2; -.
DR BioCyc; MetaCyc:MON-20313; -.
DR BRENDA; 6.2.1.53; 5121.
DR Proteomes; UP000008540; Chromosome.
DR GO; GO:0005524; F:ATP binding; ISS:JCVI.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020459; AMP-binding.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR PRINTS; PR00154; AMPBINDING.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic biosynthesis; ATP-binding; Ligase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..498
FT /note="L-proline--[L-prolyl-carrier protein] ligase"
FT /id="PRO_0000448663"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 18..22
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 30..47
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 54..60
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 63..74
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 78..81
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 88..98
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 110..115
FT /evidence="ECO:0007829|PDB:6O6E"
FT TURN 117..120
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 121..127
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 135..137
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 154..161
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 169..174
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 175..189
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 196..199
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 207..217
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 221..224
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 233..242
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 246..250
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 252..261
FT /evidence="ECO:0007829|PDB:6O6E"
FT TURN 264..266
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 274..277
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 284..293
FT /evidence="ECO:0007829|PDB:6O6E"
FT TURN 294..296
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 297..303
FT /evidence="ECO:0007829|PDB:6O6E"
FT TURN 306..308
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 312..316
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 330..332
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 337..341
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 354..360
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 365..367
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 375..385
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 391..403
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 406..409
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 410..418
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 424..433
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 434..436
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 438..444
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 453..461
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 466..468
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 471..475
FT /evidence="ECO:0007829|PDB:6O6E"
FT STRAND 485..487
FT /evidence="ECO:0007829|PDB:6O6E"
FT HELIX 489..498
FT /evidence="ECO:0007829|PDB:6O6E"
SQ SEQUENCE 498 AA; 54537 MW; 0A219EED5E35FE84 CRC64;
MKLLHERMMH SLARYPRQTA VVDEQDALSY EALELRIREF VAMLCALGVG QGQRILLWAH
KSVDLVAVMQ AALRLGVVYV PVDPLSPVSR LEKIAGDSQA VLVLCTAARL EELAGSALAQ
VRSVVLDDPA SAGYWRNIDT GSSVVPTLAI QPDDLAYILY TSGSTGVPKG VALSHGNALA
FVDWACERYC FQPGERFANH APLHFDLSVL DIYCALNVGA TVCLVPESIA FSPRLLTDFI
RQHEISIWYS VPSVLMMMMQ DGDLLSDIQD TLRVLLFAGE PFPVKHLRDL RAAYADVRLA
NLFGPTETNV CTAFEVGAID PERVLPVPIG TAASGNQVWA QKPDGSRCAV GEEGELVVQG
PTVMLGYFAK PAQEGPYKTG DMVRQRPDGN YEYLGRRDDM LKVRGNRIER GEVEAALLAH
PQVSEAAVLV VGEGMNAQLW GVLVAHTRDA LSLIDLKRHC AQRLPRYMII DKVLCLDALP
RNANGKVDRF ALARQVEG
