PLU_DROME
ID PLU_DROME Reviewed; 174 AA.
AC P42570; Q9V910;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=DNA replication inhibitor plutonium;
GN Name=plu; ORFNames=CG9183;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Iso-1;
RX PubMed=8313891; DOI=10.1002/j.1460-2075.1994.tb06281.x;
RA Axton J.M., Shamanski F.L., Young L.M., Henderson D.S., Boyd J.B.,
RA Orr-Weaver T.L.;
RT "The inhibitor of DNA replication encoded by the Drosophila gene plutonium
RT is a small, ankyrin repeat protein.";
RL EMBO J. 13:462-470(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-167, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Inhibits DNA replication early in developments. May bind and
CC block the action of a replication or initiation factor.
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DR EMBL; U03986; AAB60211.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF57492.1; -; Genomic_DNA.
DR PIR; S41713; S41713.
DR RefSeq; NP_476683.1; NM_057335.3.
DR AlphaFoldDB; P42570; -.
DR SMR; P42570; -.
DR BioGRID; 69325; 39.
DR DIP; DIP-24015N; -.
DR IntAct; P42570; 1.
DR STRING; 7227.FBpp0085618; -.
DR iPTMnet; P42570; -.
DR PaxDb; P42570; -.
DR EnsemblMetazoa; FBtr0086306; FBpp0085618; FBgn0003114.
DR GeneID; 44848; -.
DR KEGG; dme:Dmel_CG9183; -.
DR UCSC; CG9183-RA; d. melanogaster.
DR CTD; 44848; -.
DR FlyBase; FBgn0003114; plu.
DR VEuPathDB; VectorBase:FBgn0003114; -.
DR eggNOG; ENOG502T8DJ; Eukaryota.
DR HOGENOM; CLU_1751588_0_0_1; -.
DR InParanoid; P42570; -.
DR OMA; MIPALCV; -.
DR OrthoDB; 1452390at2759; -.
DR PhylomeDB; P42570; -.
DR SignaLink; P42570; -.
DR BioGRID-ORCS; 44848; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 44848; -.
DR PRO; PR:P42570; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0003114; Expressed in secondary oocyte and 9 other tissues.
DR ExpressionAtlas; P42570; baseline and differential.
DR Genevisible; P42570; DM.
DR GO; GO:1902911; C:protein kinase complex; IPI:FlyBase.
DR GO; GO:1902554; C:serine/threonine protein kinase complex; IPI:FlyBase.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0007343; P:egg activation; IMP:FlyBase.
DR GO; GO:0008156; P:negative regulation of DNA replication; IMP:FlyBase.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; TAS:FlyBase.
DR GO; GO:0007338; P:single fertilization; IMP:FlyBase.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF13637; Ank_4; 1.
DR SMART; SM00248; ANK; 2.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
PE 1: Evidence at protein level;
KW ANK repeat; DNA replication; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..174
FT /note="DNA replication inhibitor plutonium"
FT /id="PRO_0000067057"
FT REPEAT 39..68
FT /note="ANK 1"
FT REPEAT 72..103
FT /note="ANK 2"
FT MOD_RES 167
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VARIANT 97
FT /note="S -> W"
SQ SEQUENCE 174 AA; 19319 MW; E3506DD6D8BBC3FD CRC64;
MGEINALSCV GQDDVVSLRI VCTMARDGKQ HNLEDVDMYG NTALLKACYL GRFECARTLL
EFGANIFAMN YFGQNALTLA TYAGHLTLVK ELLRRRSYKD FNLSSMIPAL CVATLQKHSA
LVAYFTQLDS RGVQETQTVH GLGVAELRGM IKAAGRLDKR NVRSPPTFIS NRLR
