PLX1_PHYBI
ID PLX1_PHYBI Reviewed; 64 AA.
AC P81565; Q599T7; Q9PT74;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 04-MAY-2001, sequence version 1.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=Phylloxin-B1 {ECO:0000303|PubMed:18644413};
DE Short=PLX-B1 {ECO:0000303|PubMed:18644413};
DE AltName: Full=Phylloxin {ECO:0000303|PubMed:10632707};
DE Flags: Precursor;
GN Name=PLX-B {ECO:0000303|PubMed:15936849};
OS Phyllomedusa bicolor (Two-colored leaf frog) (Rana bicolor).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC Phyllomedusa.
OX NCBI_TaxID=8393;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 45-63, FUNCTION,
RP SUBCELLULAR LOCATION, AMIDATION AT GLN-63, AND MASS SPECTROMETRY.
RC TISSUE=Skin secretion;
RX PubMed=10632707; DOI=10.1046/j.1432-1327.2000.01012.x;
RA Pierre T.P., Seon A.A., Amiche M., Nicolas P.;
RT "Phylloxin, a novel peptide antibiotic of the dermaseptin family of
RT antimicrobial/opioid peptide precursors.";
RL Eur. J. Biochem. 267:370-378(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skin secretion;
RX PubMed=15936849; DOI=10.1016/j.peptides.2005.04.017;
RA Chen T., Gagliardo R., Walker B., Zhou M., Shaw C.;
RT "Partial structure of the phylloxin gene from the giant monkey frog,
RT Phyllomedusa bicolor: parallel cloning of precursor cDNA and genomic DNA
RT from lyophilized skin secretion.";
RL Peptides 26:2624-2628(2005).
RN [3]
RP NOMENCLATURE.
RX PubMed=18644413; DOI=10.1016/j.peptides.2008.06.017;
RA Amiche M., Ladram A., Nicolas P.;
RT "A consistent nomenclature of antimicrobial peptides isolated from frogs of
RT the subfamily Phyllomedusinae.";
RL Peptides 29:2074-2082(2008).
CC -!- FUNCTION: Antimicrobial peptide against the wall-less bacteria
CC A.laidlawii and S.melliferum, the Gram-positive bacteria B.megaterium
CC KM, C.glutamicum ATCC 27853 and M.luteus ATCC 27853 and the Gram-
CC negative-bacteria R.meliloti 102F34 and E.coli K12.
CC {ECO:0000269|PubMed:10632707}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10632707}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000305|PubMed:10632707}.
CC -!- MASS SPECTROMETRY: Mass=1980.96; Mass_error=0.1; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10632707};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Phylloxin subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC URL="https://wangapd3.com/database/query_output.php?ID=00167";
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DR EMBL; AJ251876; CAB63925.1; -; mRNA.
DR EMBL; AJ867213; CAI29483.1; -; Genomic_DNA.
DR AlphaFoldDB; P81565; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR Pfam; PF03032; FSAP_sig_propep; 1.
PE 1: Evidence at protein level;
KW Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW Cleavage on pair of basic residues; Direct protein sequencing; Immunity;
KW Innate immunity; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..42
FT /evidence="ECO:0000305|PubMed:10632707"
FT /id="PRO_0000007115"
FT PEPTIDE 45..63
FT /note="Phylloxin-B1"
FT /evidence="ECO:0000269|PubMed:10632707"
FT /id="PRO_0000007116"
FT MOD_RES 63
FT /note="Glutamine amide"
FT /evidence="ECO:0000269|PubMed:10632707"
SQ SEQUENCE 64 AA; 7256 MW; D8E5FAE8F315A618 CRC64;
MVFLKKSLLL VLFVGLVSLS ICEENKREEH EEIEENKEKA EEKRGWMSKI ASGIGTFLSG
MQQG
