PLY10_ARATH
ID PLY10_ARATH Reviewed; 440 AA.
AC Q9LJ42; Q8GXJ2;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Probable pectate lyase 10;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At3g24670; ORFNames=MSD24.10, MSD24_7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 94-440.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01216.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAC42832.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP000740; BAB01216.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76935.1; -; Genomic_DNA.
DR EMBL; AK118210; BAC42832.1; ALT_INIT; mRNA.
DR RefSeq; NP_189110.1; NM_113378.2.
DR AlphaFoldDB; Q9LJ42; -.
DR SMR; Q9LJ42; -.
DR STRING; 3702.AT3G24670.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR PaxDb; Q9LJ42; -.
DR PRIDE; Q9LJ42; -.
DR ProteomicsDB; 226187; -.
DR EnsemblPlants; AT3G24670.1; AT3G24670.1; AT3G24670.
DR GeneID; 822063; -.
DR Gramene; AT3G24670.1; AT3G24670.1; AT3G24670.
DR KEGG; ath:AT3G24670; -.
DR Araport; AT3G24670; -.
DR TAIR; locus:2093131; AT3G24670.
DR eggNOG; ENOG502QQ5F; Eukaryota.
DR HOGENOM; CLU_026608_0_1_1; -.
DR InParanoid; Q9LJ42; -.
DR OMA; EPSEGEW; -.
DR OrthoDB; 582220at2759; -.
DR PhylomeDB; Q9LJ42; -.
DR BioCyc; ARA:AT3G24670-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:Q9LJ42; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJ42; baseline and differential.
DR Genevisible; Q9LJ42; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..440
FT /note="Probable pectate lyase 10"
FT /id="PRO_0000024874"
FT REGION 32..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 318
FT /evidence="ECO:0000255"
FT BINDING 238
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 262
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 266
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 440 AA; 48761 MW; 5904FD25F5798FDD CRC64;
MVIFSRSFLA LSTTLIILAL CINSSTMAQE TEDLNSHSSS NSSTANKLPN DDGAWNEHAV
KNPEEVAAMV DMKIKNSTER RRLGFFSCAT GNPIDDCWRC DRNWHLRRKR LANCAIGFGR
NAIGGRDGRY YVVTDPSDHD AVNPRPGTLR HAVIQDRPLW IVFKRDMVIT LTQELIMNSF
KTIDGRGVNV AIAGGACITI QYVTNIIIHG INVHDCRRTG NAMVRSSPSH YGWRTMADGD
AISIFGSSHI WIDHNSLSNC ADGLIDAIMG STAITISNNY MTHHNEVMLM GHSDSYTRDK
LMQVTIAYNH FGEGLIQRMP RCRHGYFHVV NNDYTHWVMY AIGGSANPTI NSQGNRFLAP
GNPFAKEVTK RVGSWQGEWK QWNWRSQGDL MLNGAYFTKS GAAAPASYAR ASSLGAKPAS
VVSMLTYSSG ALKCRIGMRC
