PLY20_ARATH
ID PLY20_ARATH Reviewed; 417 AA.
AC Q93WF1; Q8LDB4; Q9FI81;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable pectate lyase 20;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At5g48900; ORFNames=K19E20.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10313.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB017061; BAB10313.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95740.1; -; Genomic_DNA.
DR EMBL; AY050795; AAK92730.1; -; mRNA.
DR EMBL; AY050404; AAK91420.1; -; mRNA.
DR EMBL; AY086099; AAM63307.1; -; mRNA.
DR RefSeq; NP_568705.1; NM_124267.3.
DR AlphaFoldDB; Q93WF1; -.
DR SMR; Q93WF1; -.
DR BioGRID; 20194; 1.
DR STRING; 3702.AT5G48900.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR iPTMnet; Q93WF1; -.
DR PaxDb; Q93WF1; -.
DR PRIDE; Q93WF1; -.
DR ProteomicsDB; 235044; -.
DR EnsemblPlants; AT5G48900.1; AT5G48900.1; AT5G48900.
DR GeneID; 834948; -.
DR Gramene; AT5G48900.1; AT5G48900.1; AT5G48900.
DR KEGG; ath:AT5G48900; -.
DR Araport; AT5G48900; -.
DR TAIR; locus:2154384; AT5G48900.
DR eggNOG; ENOG502QQ5F; Eukaryota.
DR HOGENOM; CLU_026608_0_1_1; -.
DR InParanoid; Q93WF1; -.
DR OMA; DKRWYLR; -.
DR OrthoDB; 582220at2759; -.
DR PhylomeDB; Q93WF1; -.
DR BioCyc; ARA:AT5G48900-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:Q93WF1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93WF1; baseline and differential.
DR Genevisible; Q93WF1; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..417
FT /note="Probable pectate lyase 20"
FT /id="PRO_0000024885"
FT ACT_SITE 295
FT /evidence="ECO:0000255"
FT BINDING 215
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 239
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 243
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 53
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 417 AA; 46728 MW; 286C71B9AE957848 CRC64;
MAVTQILVVF ASALLLSMFF TGVDSTRSNE TWHEHAVENP EEVAAMVDMS IRNSTARRRL
GYFSCSTGNP IDDCWRCDRR WQSRRKHLAN CAIGFGRNAI GGRDGRYYVV SDPNDDNPVN
PKPGTLRHAV IQEEPLWIVF KRDMVITLKE ELIMNSFKTI DGRGVNVHIA NGACITIQFV
TNIIIHGIHI HDCRPTGNAM VRSSPSHYGW RTMADGDGIS IFGSSHIWID HNSLSNCADG
LIDAVMASTA ITISNNYFTH HNEVMLLGHS DTYTRDKVMQ VTIAYNHFGE GLIQRMPRCR
HGYFHVVNND YTHWEMYAIG GSASPTINSQ GNRYLAPRNR FAKEVTKRDY AGQWQWRHWN
WRSEGDLFLN GAFFTRSGSG LGASYARASS LAAKSSSLVG VITYNAGALN CRGGRRC
