PLY4_ARATH
ID PLY4_ARATH Reviewed; 368 AA.
AC Q9C8G4; Q1PFQ4;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Probable pectate lyase 4;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At1g30350; ORFNames=T4K22.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
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DR EMBL; AC025295; AAG51103.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31208.1; -; Genomic_DNA.
DR EMBL; DQ446309; ABE65673.1; -; mRNA.
DR PIR; G86427; G86427.
DR RefSeq; NP_174324.1; NM_102772.3.
DR AlphaFoldDB; Q9C8G4; -.
DR SMR; Q9C8G4; -.
DR STRING; 3702.AT1G30350.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR PaxDb; Q9C8G4; -.
DR PRIDE; Q9C8G4; -.
DR ProteomicsDB; 234678; -.
DR EnsemblPlants; AT1G30350.1; AT1G30350.1; AT1G30350.
DR GeneID; 839915; -.
DR Gramene; AT1G30350.1; AT1G30350.1; AT1G30350.
DR KEGG; ath:AT1G30350; -.
DR Araport; AT1G30350; -.
DR TAIR; locus:2204232; AT1G30350.
DR eggNOG; ENOG502QSYA; Eukaryota.
DR HOGENOM; CLU_026608_0_1_1; -.
DR InParanoid; Q9C8G4; -.
DR OMA; DACWRRN; -.
DR OrthoDB; 924221at2759; -.
DR PhylomeDB; Q9C8G4; -.
DR BioCyc; ARA:AT1G30350-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:Q9C8G4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C8G4; baseline and differential.
DR Genevisible; Q9C8G4; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..368
FT /note="Probable pectate lyase 4"
FT /id="PRO_0000024868"
FT ACT_SITE 247
FT /evidence="ECO:0000255"
FT BINDING 167
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 191
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 195
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 368 AA; 40576 MW; F35EDEDB2CED34BC CRC64;
MASLVVIVSL LLAAFASPLL ETAHSYNVTA PRVSLNPIDA CWRRNPKWAT NRQALAHCAV
GYGKAAIGGK NGPIYVVTNP SDNPTRPSPG TLRYAVSQPK PLWITFARDM VIVLKSQLMI
NSYKTIDGRG AKVEIANGPC LRIRQVKHVI IHGISIHDCK ADPNGMDGDG IRVFQSTHVW
IDHCFLSRCH DGLIDVIVSS TAVTISNNYF TQHDKVMLLG HDDSYMGDKD MRVTIAFNTF
GPGLIERMPR VRRGYAHVAN NRYEKWQMYA IGGSANPIIF SEGNYFVAPE KRSSKQVTKR
MMAGPDSKRW KWGTSRDVFM NGAFFGPPGV IVRPLYKGGE GFRVAHGSLV PSLTSSAGPL
RCYVGRIC
