PLY6_ARATH
ID PLY6_ARATH Reviewed; 455 AA.
AC O64510;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Probable pectate lyase 6;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At2g02720; ORFNames=T20F6.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
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DR EMBL; AC002521; AAC05350.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05615.1; -; Genomic_DNA.
DR EMBL; BT005757; AAO64162.1; -; mRNA.
DR PIR; T00856; T00856.
DR RefSeq; NP_178375.1; NM_126327.4.
DR AlphaFoldDB; O64510; -.
DR SMR; O64510; -.
DR STRING; 3702.AT2G02720.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR PaxDb; O64510; -.
DR PRIDE; O64510; -.
DR ProteomicsDB; 234929; -.
DR EnsemblPlants; AT2G02720.1; AT2G02720.1; AT2G02720.
DR GeneID; 814801; -.
DR Gramene; AT2G02720.1; AT2G02720.1; AT2G02720.
DR KEGG; ath:AT2G02720; -.
DR Araport; AT2G02720; -.
DR TAIR; locus:2058842; AT2G02720.
DR eggNOG; ENOG502QQE2; Eukaryota.
DR HOGENOM; CLU_026608_2_1_1; -.
DR InParanoid; O64510; -.
DR OMA; CKEYVKC; -.
DR OrthoDB; 924221at2759; -.
DR PhylomeDB; O64510; -.
DR BioCyc; ARA:AT2G02720-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:O64510; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64510; baseline and differential.
DR Genevisible; O64510; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR007524; Pec_lyase_N.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF04431; Pec_lyase_N; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..455
FT /note="Probable pectate lyase 6"
FT /id="PRO_0000024870"
FT ACT_SITE 327
FT /evidence="ECO:0000255"
FT BINDING 247
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 271
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 275
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 455 AA; 51258 MW; 7C6A35A767A30CA4 CRC64;
MVNLGSYVFV FVALSLTVVV PSVQAHIAEY DEYWTQRQTN ALRETLESYD PNPENVTDHF
NYHAALAMET TGIVNETRRD LRQVGRGKKT TRRGGRFESL NAIDKCWRGD KNWDKNRKKL
ADCVLGFGRK TTGGKNGPIY VVTDPSDNDL LKPKPGTIRH AVTRDRPLWI IFARSMIIKL
QQELIITNDK TIDGRGAKIY ITGGAGLTLQ FVRNVIIHNI HIKQIKRGAG GLIIDSEQHF
GLRTVSDGDG INIFGATNVW IDHVSMTDCS DGMIDAIMGS TAITISNSHF TDHDEVMLFG
GTNKDVIDKK MQITVAFNHF GKRLKQRMPR VRFGLVHVVN NDYTHWEMYA IGGNMNPTII
SQGNRFIAPP IEDSKQVTKR EYTPYPEWKS WNWQSEKDYF LNGAYFVQSG KANAWSATPK
NPIPRKFAIR PQPGTKVRRL TKDAGTLGCK PGKSC
