PLY7_ARATH
ID PLY7_ARATH Reviewed; 475 AA.
AC Q9SRH4; Q8LGC8;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Probable pectate lyase 7;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At3g01270; ORFNames=T22N4.10, T4P13.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
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DR EMBL; AC008261; AAF26147.1; -; Genomic_DNA.
DR EMBL; AC010676; AAF03499.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73631.1; -; Genomic_DNA.
DR EMBL; AY084341; AAM60924.1; -; mRNA.
DR RefSeq; NP_186776.1; NM_110993.4.
DR AlphaFoldDB; Q9SRH4; -.
DR SMR; Q9SRH4; -.
DR STRING; 3702.AT3G01270.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR PaxDb; Q9SRH4; -.
DR PRIDE; Q9SRH4; -.
DR ProteomicsDB; 235048; -.
DR EnsemblPlants; AT3G01270.1; AT3G01270.1; AT3G01270.
DR GeneID; 821142; -.
DR Gramene; AT3G01270.1; AT3G01270.1; AT3G01270.
DR KEGG; ath:AT3G01270; -.
DR Araport; AT3G01270; -.
DR TAIR; locus:2100247; AT3G01270.
DR eggNOG; ENOG502QQE2; Eukaryota.
DR HOGENOM; CLU_026608_0_1_1; -.
DR InParanoid; Q9SRH4; -.
DR OMA; CQELMIN; -.
DR OrthoDB; 924221at2759; -.
DR PhylomeDB; Q9SRH4; -.
DR BioCyc; ARA:AT3G01270-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:Q9SRH4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SRH4; baseline and differential.
DR Genevisible; Q9SRH4; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR007524; Pec_lyase_N.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF04431; Pec_lyase_N; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..475
FT /note="Probable pectate lyase 7"
FT /id="PRO_0000024871"
FT REGION 91..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 351
FT /evidence="ECO:0000255"
FT BINDING 271
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 295
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 299
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 28
FT /note="D -> E (in Ref. 3; AAM60924)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="V -> I (in Ref. 3; AAM60924)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 475 AA; 53855 MW; DE1550EA09B8161D CRC64;
METARLFKLV CVICIASLIP TIRANVADET DEYWVNKANE ARKHTLMAYH PDPYEIVDHF
HERHYDNSTD VEGTEEEKAV ASEEEDVIEM ISSPTNSTRR SLTGRGKGKG KGKWSKLTGP
CTASNPIDKC WRCQPDWARR RKKLVHCVRG FGYRTTGGKR GRIYVVTSPR DDDMVNPRPG
TLRHAVIQKE PLWIVFKHDM SIRLSQELMI TSDKTIDARG ANVHIAYGAG ITMQYVHNII
IHGLHVHHIV KSSGGLIRDS INHFGHRGEA DGDGISIFGA TNIWLDHISM SKCQDGLIDA
IMGSTAITIS NSHFTHHNDV MLLGAQNNNM DDKKMQVTVA YNHFGKGLVQ RMPRVRWGFV
HVVNNDYTHW ELYAIGGSQG PTILSHGNRF IAPPHKQHYR EVTKRDYASE SEWKNWNWRS
EKDVFMNNAY FRQSGNPHFK CSHSRQQMIK PKNGMAVSKL TKYAGALDCR VGKAC
