PLY8_ARATH
ID PLY8_ARATH Reviewed; 416 AA.
AC Q9M8Z8; Q8LF66;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable pectate lyase 8;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At3g07010; ORFNames=F17A9.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
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DR EMBL; AC016827; AAF27005.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74488.1; -; Genomic_DNA.
DR EMBL; AY085026; AAM61584.1; -; mRNA.
DR RefSeq; NP_187357.1; NM_111581.3.
DR AlphaFoldDB; Q9M8Z8; -.
DR SMR; Q9M8Z8; -.
DR BioGRID; 5221; 1.
DR STRING; 3702.AT3G07010.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR iPTMnet; Q9M8Z8; -.
DR PaxDb; Q9M8Z8; -.
DR PRIDE; Q9M8Z8; -.
DR ProteomicsDB; 234976; -.
DR EnsemblPlants; AT3G07010.1; AT3G07010.1; AT3G07010.
DR GeneID; 819886; -.
DR Gramene; AT3G07010.1; AT3G07010.1; AT3G07010.
DR KEGG; ath:AT3G07010; -.
DR Araport; AT3G07010; -.
DR TAIR; locus:2077622; AT3G07010.
DR eggNOG; ENOG502QQ5F; Eukaryota.
DR HOGENOM; CLU_026608_0_1_1; -.
DR InParanoid; Q9M8Z8; -.
DR OrthoDB; 582220at2759; -.
DR PhylomeDB; Q9M8Z8; -.
DR BioCyc; ARA:AT3G07010-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:Q9M8Z8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M8Z8; baseline and differential.
DR Genevisible; Q9M8Z8; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..416
FT /note="Probable pectate lyase 8"
FT /id="PRO_0000024872"
FT ACT_SITE 294
FT /evidence="ECO:0000255"
FT BINDING 214
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 238
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 242
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 28
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 17
FT /note="A -> T (in Ref. 3; AAM61584)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="G -> C (in Ref. 3; AAM61584)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="A -> S (in Ref. 3; AAM61584)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 46174 MW; 27BBCB0FB6B047C2 CRC64;
MAVTKLILFA SALLLTALFI GVNASRSNET WHEHAVENPD EVAAMVDMSI RNSTERRRLG
YFSCATGNPI DDCWRCDRKW QLRRKRLADC SIGFGRNAIG GRDGRFYVVT DPGDDDPVNP
IPGTLRHAVI QDEPLWIIFK RDMVITLKQE LIMNSFKTID GRGVNVHIAN GACLTIQYVT
NIIVHGIHVH DCKPTGNAMV RSSPSHYGFR SMADGDAISI FGSSHIWIDH NSLSNCADGL
VDAVMSSTAI TVSNNFFTHH NEVMLLGHSD SYTRDKVMQV TIAYNHFGEG LIQRMPRCRH
GYFHVVNNDY THWEMYAIGG SAGPTINSQG NRFLAPVNPF AKEVTKREYT GESKWKHWNW
RSEGDLFLNG AFFTRSGAGA GANYARASSL SAKSSSLVGT MTSYSGALNC RAGRRC
