PLY9_ARATH
ID PLY9_ARATH Reviewed; 452 AA.
AC Q9LRM5;
DT 16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Probable pectate lyase 9;
DE EC=4.2.2.2;
DE Flags: Precursor;
GN OrderedLocusNames=At3g24230; ORFNames=MUJ8.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC gluconate from pectin: step 2/5.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC {ECO:0000305}.
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DR EMBL; AB028621; BAB01365.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76877.1; -; Genomic_DNA.
DR EMBL; BX823056; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_189065.2; NM_113328.3.
DR AlphaFoldDB; Q9LRM5; -.
DR SMR; Q9LRM5; -.
DR STRING; 3702.AT3G24230.1; -.
DR CAZy; PL1; Polysaccharide Lyase Family 1.
DR PaxDb; Q9LRM5; -.
DR PRIDE; Q9LRM5; -.
DR EnsemblPlants; AT3G24230.1; AT3G24230.1; AT3G24230.
DR GeneID; 822010; -.
DR Gramene; AT3G24230.1; AT3G24230.1; AT3G24230.
DR KEGG; ath:AT3G24230; -.
DR Araport; AT3G24230; -.
DR TAIR; locus:2093761; AT3G24230.
DR eggNOG; ENOG502QQ5F; Eukaryota.
DR HOGENOM; CLU_026608_0_1_1; -.
DR InParanoid; Q9LRM5; -.
DR OMA; HMSEQNI; -.
DR OrthoDB; 582220at2759; -.
DR PhylomeDB; Q9LRM5; -.
DR BioCyc; ARA:AT3G24230-MON; -.
DR UniPathway; UPA00545; UER00824.
DR PRO; PR:Q9LRM5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRM5; baseline and differential.
DR Genevisible; Q9LRM5; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR018082; AmbAllergen.
DR InterPro; IPR002022; Pec_lyase.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045032; PEL.
DR PANTHER; PTHR31683; PTHR31683; 1.
DR Pfam; PF00544; Pectate_lyase_4; 1.
DR PRINTS; PR00807; AMBALLERGEN.
DR SMART; SM00656; Amb_all; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..452
FT /note="Probable pectate lyase 9"
FT /id="PRO_0000024873"
FT ACT_SITE 330
FT /evidence="ECO:0000255"
FT BINDING 250
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 274
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 278
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 214
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 233
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 271
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 281
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 305
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 374
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 224
FT /note="H -> N (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
FT CONFLICT 306
FT /note="D -> H (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
FT CONFLICT 326
FT /note="G -> A (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
FT CONFLICT 338
FT /note="Y -> H (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
FT CONFLICT 346
FT /note="Y -> H (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
FT CONFLICT 408
FT /note="F -> S (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
FT CONFLICT 419
FT /note="D -> H (in Ref. 3; BX823056)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 452 AA; 49985 MW; F31AD9097F923249 CRC64;
MATSSLKLTS ACFVLLFIFV GCVLTATNLR NNEISRSRKL KTEDSKSFNS SPMTTRLDGV
VELNEHAVTD PDKVAHEVSN LIHMSEQNIT ARRKLGFFSC GNGNLIDDCW RCDRNWNKNR
KHLADCGMGF GSKAFGGRNG SYYVVTDHSD DDVVNPKPGT LRHAVIQVEP LWIIFKRDMV
IKLKQELIMN SFKTIDARGA NVHIANGACI TIQNITNVIV HGLHIHDCKR TGNVTVRSSP
SQAGFRGTAD GDAINIFGSS HIWIDHNSLS NCTDGLVDVV NGSTAITISN NHFTHHDEVM
LLGHNDSYTR DKMMQVTVAY NHFGEGLIQR MPRCRHGYFH VVNNDYTHWK MYAIGGSANP
TINSQGNRFA APKNHSAKEV TKRLDTKGNE WMEWNWRSEK DLLVNGAFFT PSGEGASGDS
QTLSLPAKPA SMVDAITASA GALSCRRGKP CY
