AT101_ARATH
ID AT101_ARATH Reviewed; 215 AA.
AC F4K265; Q84WU3; Q93ZX0; Q9FKY8;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Autophagy-related protein 101 {ECO:0000303|PubMed:24563201};
GN Name=ATG101 {ECO:0000303|PubMed:24563201};
GN OrderedLocusNames=At5g66930 {ECO:0000312|Araport:AT5G66930};
GN ORFNames=MUD21.19 {ECO:0000312|EMBL:BAB08635.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP INTERACTION WITH ATG11 AND ATG13A, AND SUBCELLULAR LOCATION.
RX PubMed=24563201; DOI=10.1105/tpc.113.120014;
RA Li F., Chung T., Vierstra R.D.;
RT "AUTOPHAGY-RELATED11 plays a critical role in general autophagy- and
RT senescence-induced mitophagy in Arabidopsis.";
RL Plant Cell 26:788-807(2014).
CC -!- FUNCTION: Accessory protein involved in autophagy. Acts as scaffold
CC protein of the ATG1-ATG13 complex for faithful delivery of autophagic
CC vesicles to the vacuole. Required for selective mitophagy.
CC {ECO:0000250|UniProtKB:Q9SUG7}.
CC -!- SUBUNIT: Interacts with ATG11 and ATG13A.
CC {ECO:0000269|PubMed:24563201}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome
CC {ECO:0000269|PubMed:24563201}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4K265-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4K265-2; Sequence=VSP_057968, VSP_057969;
CC -!- SIMILARITY: Belongs to the ATG101 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08635.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX833047; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB010700; BAB08635.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98279.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98280.1; -; Genomic_DNA.
DR EMBL; AY056211; AAL07060.1; -; mRNA.
DR EMBL; BT002759; AAO22587.1; -; mRNA.
DR EMBL; BX833047; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_569042.2; NM_126092.5. [F4K265-1]
DR RefSeq; NP_851284.1; NM_180953.2. [F4K265-2]
DR AlphaFoldDB; F4K265; -.
DR SMR; F4K265; -.
DR IntAct; F4K265; 1.
DR STRING; 3702.AT5G66930.3; -.
DR iPTMnet; F4K265; -.
DR PaxDb; F4K265; -.
DR PRIDE; F4K265; -.
DR ProteomicsDB; 246522; -. [F4K265-1]
DR EnsemblPlants; AT5G66930.1; AT5G66930.1; AT5G66930. [F4K265-2]
DR EnsemblPlants; AT5G66930.2; AT5G66930.2; AT5G66930. [F4K265-1]
DR GeneID; 836827; -.
DR Gramene; AT5G66930.1; AT5G66930.1; AT5G66930. [F4K265-2]
DR Gramene; AT5G66930.2; AT5G66930.2; AT5G66930. [F4K265-1]
DR KEGG; ath:AT5G66930; -.
DR Araport; AT5G66930; -.
DR eggNOG; KOG4493; Eukaryota.
DR HOGENOM; CLU_069661_0_0_1; -.
DR OMA; VCWEIWT; -.
DR PRO; PR:F4K265; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K265; baseline and differential.
DR Genevisible; F4K265; AT.
DR GO; GO:0005776; C:autophagosome; IEA:UniProtKB-SubCell.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0000407; C:phagophore assembly site; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR012445; ATG101.
DR PANTHER; PTHR13292; PTHR13292; 1.
DR Pfam; PF07855; ATG101; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Autophagy; Cytoplasmic vesicle; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..215
FT /note="Autophagy-related protein 101"
FT /id="PRO_0000434629"
FT REGION 124..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 139..157
FT /note="EASEERSSRRTLLEQSLQE -> GSIDQFPVLISKNYIFLLL (in
FT isoform 2)"
FT /id="VSP_057968"
FT VAR_SEQ 158..215
FT /note="Missing (in isoform 2)"
FT /id="VSP_057969"
FT CONFLICT 82
FT /note="K -> E (in Ref. 3; AAL07060)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 215 AA; 25278 MW; 9721238C4715AE7D CRC64;
MNCEVCQLKE LEVESFEIRE VLRCILHTIV FHRALGLIRP KDIDLELFEI TYVQCGEIEV
EKKIDEKIEQ FINWIEKHPN KKSQICLSFY EVKSKQPSWF TKIERLYWEQ WYINLNVLQP
TKPPVGKSHH SKLVMDPGEA SEERSSRRTL LEQSLQEVLF QIIKFVNEKK DHVPPINDGV
IYYPFEITIP SSSDSAFGMD MFKRILHSGH PSMLG
