ID   PMBA_ECO57              Reviewed;         450 AA.
AC   P0AFK1; P24231;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Metalloprotease PmbA;
DE            EC=3.4.-.-;
GN   Name=pmbA; OrderedLocusNames=Z5845, ECs5212;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Metalloprotease involved in CcdA degradation. Suppresses the
CC       inhibitory activity of the carbon storage regulator (CsrA) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase U62 family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG59432.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38635.1; -; Genomic_DNA.
DR   PIR; D86121; D86121.
DR   PIR; D91280; D91280.
DR   RefSeq; NP_313239.1; NC_002695.1.
DR   RefSeq; WP_001162171.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AFK1; -.
DR   SMR; P0AFK1; -.
DR   STRING; 155864.EDL933_5582; -.
DR   EnsemblBacteria; AAG59432; AAG59432; Z5845.
DR   EnsemblBacteria; BAB38635; BAB38635; ECs_5212.
DR   GeneID; 60903082; -.
DR   GeneID; 913868; -.
DR   KEGG; ece:Z5845; -.
DR   KEGG; ecs:ECs_5212; -.
DR   PATRIC; fig|386585.9.peg.5448; -.
DR   eggNOG; COG0312; Bacteria.
DR   HOGENOM; CLU_026425_0_0_6; -.
DR   OMA; LYRKSTF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2290.10; -; 1.
DR   InterPro; IPR045569; Metalloprtase-TldD/E_C.
DR   InterPro; IPR045570; Metalloprtase-TldD/E_cen_dom.
DR   InterPro; IPR002510; Metalloprtase-TldD/E_N.
DR   InterPro; IPR035068; TldD/PmbA_N.
DR   InterPro; IPR036059; TldD/PmbA_sf.
DR   Pfam; PF01523; PmbA_TldD; 1.
DR   Pfam; PF19289; PmbA_TldD_C; 1.
DR   Pfam; PF19290; PmbA_TldD_M; 1.
DR   SUPFAM; SSF111283; SSF111283; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Metalloprotease; Protease; Reference proteome.
FT   CHAIN           1..450
FT                   /note="Metalloprotease PmbA"
FT                   /id="PRO_0000142352"
SQ   SEQUENCE   450 AA;  48370 MW;  A062969197B52B5E CRC64;
     MALAMKVISQ VEAQRKILEE AVSTALELAS GKSDGAEVAV SKTTGISVST RYGEVENVEF
     NSDGALGITV YHQNRKGSAS STDLSPQAIA RTVQAALDIA RYTSPDPCAG VADKELLAFD
     APDLDLFHPA EVSPDEAIEL AARAEQAALQ ADKRITNTEG GSFNSHYGVK VFGNSHGMLQ
     GYCSTRHSLS SCVIAEENGD MERDYAYTIG RAMSDLQTPE WVGADCARRT LSRLSPRKLS
     TMKAPVIFAN EVATGLFGHL VGAIAGGSVY RKSTFLLDSL GKQILPDWLT IEEHPHLLKG
     LASTPFDSEG VRTERRDIIK DGILTQWLLT SYSARKLGLK STGHAGGIHN WRIAGQGLSF
     EQMLKEMGTG LVVTELMGQG VSAITGDYSR GAAGFWVENG EIQYPVSEIT IAGNLKDMWR
     NIVTVGNDIE TRSNIQCGSV LLPEMKIAGQ