AT12A_ARATH
ID AT12A_ARATH Reviewed; 96 AA.
AC Q8S924;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ubiquitin-like protein ATG12A;
DE AltName: Full=Autophagy-related protein 12a;
DE Short=APG12-like protein a;
DE Short=AtAPG12a;
GN Name=ATG12A; Synonyms=APG12A; OrderedLocusNames=At1g54210;
GN ORFNames=F20D21.38;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12070171; DOI=10.1074/jbc.m204630200;
RA Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.;
RT "The APG8/12-activating enzyme APG7 is required for proper nutrient
RT recycling and senescence in Arabidopsis thaliana.";
RL J. Biol. Chem. 277:33105-33114(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, AND GENE FAMILY.
RX PubMed=12114572; DOI=10.1104/pp.011024;
RA Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D.,
RA Tabata S., Ohsumi Y.;
RT "Leaf senescence and starvation-induced chlorosis are accelerated by the
RT disruption of an Arabidopsis autophagy gene.";
RL Plant Physiol. 129:1181-1193(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16040659; DOI=10.1104/pp.105.060673;
RA Thompson A.R., Doelling J.H., Suttangkakul A., Vierstra R.D.;
RT "Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and
RT ATG12 conjugation pathways.";
RL Plant Physiol. 138:2097-2110(2005).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Ubiquitin-like protein involved in cytoplasm to vacuole
CC transport (Cvt) and autophagy vesicles formation. Conjugation with ATG5
CC through a ubiquitin-like conjugating system involving also ATG7 as an
CC E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme,
CC is essential for its function. ATG12/ATG5 conjugate has an essential
CC role in plant nutrient recycling. {ECO:0000269|PubMed:16040659}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8S924-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16040659}.
CC -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR EMBL; AF492758; AAM70187.1; -; mRNA.
DR EMBL; AB073184; BAB88396.1; -; mRNA.
DR EMBL; AC005287; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE33061.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57757.1; -; Genomic_DNA.
DR EMBL; BT024654; ABD57479.1; -; mRNA.
DR RefSeq; NP_001320241.1; NM_001333633.1. [Q8S924-1]
DR RefSeq; NP_175823.1; NM_104299.3. [Q8S924-1]
DR AlphaFoldDB; Q8S924; -.
DR SMR; Q8S924; -.
DR IntAct; Q8S924; 1.
DR MINT; Q8S924; -.
DR STRING; 3702.AT1G54210.1; -.
DR TCDB; 9.A.15.3.1; the autophagy-related phagophore-formation transporter (apt) family.
DR iPTMnet; Q8S924; -.
DR PaxDb; Q8S924; -.
DR PRIDE; Q8S924; -.
DR DNASU; 841861; -.
DR EnsemblPlants; AT1G54210.1; AT1G54210.1; AT1G54210. [Q8S924-1]
DR EnsemblPlants; AT1G54210.3; AT1G54210.3; AT1G54210. [Q8S924-1]
DR GeneID; 841861; -.
DR Gramene; AT1G54210.1; AT1G54210.1; AT1G54210. [Q8S924-1]
DR Gramene; AT1G54210.3; AT1G54210.3; AT1G54210. [Q8S924-1]
DR KEGG; ath:AT1G54210; -.
DR Araport; AT1G54210; -.
DR TAIR; locus:2020098; AT1G54210.
DR eggNOG; KOG3439; Eukaryota.
DR HOGENOM; CLU_106795_3_1_1; -.
DR InParanoid; Q8S924; -.
DR OMA; LFIYVHQ; -.
DR PhylomeDB; Q8S924; -.
DR PRO; PR:Q8S924; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8S924; baseline and differential.
DR Genevisible; Q8S924; AT.
DR GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; IBA:GO_Central.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; IGI:TAIR.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0006501; P:C-terminal protein lipidation; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR007242; Atg12.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR13385; PTHR13385; 1.
DR Pfam; PF04110; APG12; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Autophagy; Cytoplasm; Isopeptide bond;
KW Protein transport; Reference proteome; Transport; Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..96
FT /note="Ubiquitin-like protein ATG12A"
FT /id="PRO_0000250542"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CROSSLNK 96
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-128 in ATG5)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 96 AA; 10571 MW; A2D770829EDC39B4 CRC64;
MATESSSPSS VRKVVVHLRA TGGAPILKQS KFKIPGTDKF AKVIDFLRRQ LHSDSLFVYV
NSAFSPNPDE SVIDLYNNFG FDGKLVVNYA CSMAWG
