PMEI5_ARATH
ID PMEI5_ARATH Reviewed; 179 AA.
AC Q9SIC6; Q7XJ30;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Pectinesterase inhibitor 5 {ECO:0000305};
DE AltName: Full=Pectin methylesterase inhibitor 5 {ECO:0000303|PubMed:23129203};
DE Short=AtPMEI5 {ECO:0000303|PubMed:23129203};
DE Flags: Precursor;
GN Name=PMEI5 {ECO:0000303|PubMed:23129203};
GN OrderedLocusNames=At2g31430 {ECO:0000312|Araport:AT2G31430};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23129203; DOI=10.1104/pp.112.205724;
RA Mueller K., Levesque-Tremblay G., Bartels S., Weitbrecht K., Wormit A.,
RA Usadel B., Haughn G., Kermode A.R.;
RT "Demethylesterification of cell wall pectins in Arabidopsis plays a role in
RT seed germination.";
RL Plant Physiol. 161:305-316(2013).
CC -!- FUNCTION: Pectin methylesterase (PME) inhibitor that targets PME from
CC seeds and modulates PME activity and pectin methylesterification during
CC seed germination. {ECO:0000269|PubMed:23129203}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250|UniProtKB:Q9STY5}.
CC -!- TISSUE SPECIFICITY: Expressed in seeds, buds, and mature flowers.
CC {ECO:0000269|PubMed:23129203}.
CC -!- SIMILARITY: Belongs to the PMEI family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007169; AAD26476.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08546.1; -; Genomic_DNA.
DR EMBL; AY327264; AAP92518.1; -; mRNA.
DR EMBL; AY327265; AAP92519.1; -; mRNA.
DR EMBL; AY773855; AAV63884.1; -; mRNA.
DR PIR; F84720; F84720.
DR RefSeq; NP_180701.1; NM_128700.4.
DR AlphaFoldDB; Q9SIC6; -.
DR SMR; Q9SIC6; -.
DR STRING; 3702.AT2G31430.1; -.
DR PaxDb; Q9SIC6; -.
DR PRIDE; Q9SIC6; -.
DR ProteomicsDB; 236647; -.
DR EnsemblPlants; AT2G31430.1; AT2G31430.1; AT2G31430.
DR GeneID; 817701; -.
DR Gramene; AT2G31430.1; AT2G31430.1; AT2G31430.
DR KEGG; ath:AT2G31430; -.
DR Araport; AT2G31430; -.
DR TAIR; locus:2061275; AT2G31430.
DR eggNOG; ENOG502SR8P; Eukaryota.
DR HOGENOM; CLU_1505481_0_0_1; -.
DR InParanoid; Q9SIC6; -.
DR OMA; CTATIFL; -.
DR OrthoDB; 1198655at2759; -.
DR PhylomeDB; Q9SIC6; -.
DR PRO; PR:Q9SIC6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SIC6; baseline and differential.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030599; F:pectinesterase activity; IBA:GO_Central.
DR GO; GO:0046910; F:pectinesterase inhibitor activity; IDA:UniProtKB.
DR GO; GO:0010029; P:regulation of seed germination; IMP:UniProtKB.
DR CDD; cd15797; PMEI; 1.
DR Gene3D; 1.20.140.40; -; 1.
DR InterPro; IPR035513; Invertase/methylesterase_inhib.
DR InterPro; IPR006501; Pectinesterase_inhib_dom.
DR InterPro; IPR034086; PMEI_plant.
DR Pfam; PF04043; PMEI; 1.
DR SMART; SM00856; PMEI; 1.
DR SUPFAM; SSF101148; SSF101148; 1.
DR TIGRFAMs; TIGR01614; PME_inhib; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..179
FT /note="Pectinesterase inhibitor 5"
FT /id="PRO_5008430337"
FT DISULFID 35..44
FT /evidence="ECO:0000250|UniProtKB:Q9LNF2"
FT DISULFID 101..141
FT /evidence="ECO:0000250|UniProtKB:Q9LNF2"
SQ SEQUENCE 179 AA; 20332 MW; 8F39904E195F43F7 CRC64;
MATMLINHML FLTSLLIVVF PVANAIPARD IDKLCKETTD VPFCLKYLGT DPRIPAARDL
TDVLLIAITQ SKMQVDDATT HIDRVRRKFN GPHGRRRIEV CKTNYGIASA RFHTAWELGL
QKSFWDVEKL ARIGTNAVID CENVWRRDGP IQTSPLTFYN MNVFKLSGII LLIFNKLVT
