PMEI6_ARATH
ID PMEI6_ARATH Reviewed; 208 AA.
AC O22244; Q8LCK2;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Pectinesterase inhibitor 6 {ECO:0000305};
DE AltName: Full=Pectin methylesterase inhibitor 6 {ECO:0000303|PubMed:23362209};
DE Short=AtPMEI6 {ECO:0000305};
DE Flags: Precursor;
GN Name=PMEI6 {ECO:0000303|PubMed:23362209};
GN OrderedLocusNames=At2g47670 {ECO:0000312|Araport:AT2G47670};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=23362209; DOI=10.1105/tpc.112.106575;
RA Saez-Aguayo S., Ralet M.C., Berger A., Botran L., Ropartz D.,
RA Marion-Poll A., North H.M.;
RT "Pectin methylesterase inhibitor6 promotes Arabidopsis mucilage release by
RT limiting methylesterification of homogalacturonan in seed coat epidermal
RT cells.";
RL Plant Cell 25:308-323(2013).
CC -!- FUNCTION: Pectin methylesterase (PME) inhibitor that targets PME from
CC seeds and modulates PME activity and pectin methylesterification during
CC seed germination. Promotes mucilage release by limiting
CC methylesterification of homogalacturonan in seed coat epidermal cells.
CC {ECO:0000269|PubMed:23362209}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250|UniProtKB:Q9STY5}.
CC -!- SIMILARITY: Belongs to the PMEI family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC002535; AAM14850.1; -; Genomic_DNA.
DR EMBL; AC005309; AAC63623.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10874.1; -; Genomic_DNA.
DR EMBL; AY086547; AAM63611.1; -; mRNA.
DR PIR; T00417; T00417.
DR RefSeq; NP_182289.1; NM_130335.3.
DR AlphaFoldDB; O22244; -.
DR SMR; O22244; -.
DR STRING; 3702.AT2G47670.1; -.
DR PaxDb; O22244; -.
DR PRIDE; O22244; -.
DR ProteomicsDB; 234683; -.
DR EnsemblPlants; AT2G47670.1; AT2G47670.1; AT2G47670.
DR GeneID; 819380; -.
DR Gramene; AT2G47670.1; AT2G47670.1; AT2G47670.
DR KEGG; ath:AT2G47670; -.
DR Araport; AT2G47670; -.
DR TAIR; locus:2043398; AT2G47670.
DR eggNOG; ENOG502S061; Eukaryota.
DR HOGENOM; CLU_033761_0_2_1; -.
DR InParanoid; O22244; -.
DR OMA; NAYKGYD; -.
DR OrthoDB; 1300615at2759; -.
DR PhylomeDB; O22244; -.
DR PRO; PR:O22244; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22244; baseline and differential.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004857; F:enzyme inhibitor activity; IBA:GO_Central.
DR GO; GO:0046910; F:pectinesterase inhibitor activity; IDA:TAIR.
DR GO; GO:0043086; P:negative regulation of catalytic activity; IBA:GO_Central.
DR GO; GO:0009827; P:plant-type cell wall modification; IMP:TAIR.
DR GO; GO:0010214; P:seed coat development; IMP:TAIR.
DR Gene3D; 1.20.140.40; -; 1.
DR InterPro; IPR035513; Invertase/methylesterase_inhib.
DR InterPro; IPR006501; Pectinesterase_inhib_dom.
DR Pfam; PF04043; PMEI; 1.
DR SMART; SM00856; PMEI; 1.
DR SUPFAM; SSF101148; SSF101148; 1.
DR TIGRFAMs; TIGR01614; PME_inhib; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..208
FT /note="Pectinesterase inhibitor 6"
FT /id="PRO_5008422467"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 53..62
FT /evidence="ECO:0000250|UniProtKB:Q9LNF2"
FT DISULFID 118..165
FT /evidence="ECO:0000250|UniProtKB:Q9LNF2"
FT CONFLICT 83
FT /note="A -> G (in Ref. 3; AAM63611)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="V -> M (in Ref. 3; AAM63611)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="S -> R (in Ref. 3; AAM63611)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 208 AA; 23178 MW; FBAB665AD4517287 CRC64;
MTSSSSSPIT FTLLLLLSLL VALNPNPSLA STGSNINTND IVTQYSTYVR NACNVTRYNR
LCVRTLWPFA IVARNNTSKW ARASVAVTIT DTKRVLRLLL KTQRSAVGES ERIALSDCRE
LFVDSLDNLY KSLAVLRTLN ADEFQRQISD LATWLSAALT DDDTCLDGFE ETSSRTRTVR
MVRRKATKCM RLCSNALALL KKLAFDGL
