ID   PMEPA_XENLA             Reviewed;         285 AA.
AC   D2KUZ7; A9ZS93;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Protein TMEPAI;
DE   AltName: Full=Prostate transmembrane protein androgen induced 1;
DE   AltName: Full=Transmembrane prostate androgen-induced protein;
GN   Name=pmepa1; Synonyms=tmepai;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=20129061; DOI=10.1016/j.molcel.2009.10.028;
RA   Watanabe Y., Itoh S., Goto T., Ohnishi E., Inamitsu M., Itoh F., Satoh K.,
RA   Wiercinska E., Yang W., Shi L., Tanaka A., Nakano N., Mommaas A.M.,
RA   Shibuya H., Ten Dijke P., Kato M.;
RT   "TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad
RT   proteins from active participation in TGF-beta signaling.";
RL   Mol. Cell 37:123-134(2010).
CC   -!- FUNCTION: May function as a negative regulator of TGF-beta signaling
CC       and thereby inhibits activin-mediated mesoderm formation.
CC       {ECO:0000269|PubMed:20129061}.
CC   -!- SUBCELLULAR LOCATION: Early endosome membrane {ECO:0000250}; Single-
CC       pass membrane protein {ECO:0000250}. Golgi apparatus membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PMEPA1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF96587.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB365488; BAF96587.1; ALT_INIT; mRNA.
DR   EMBL; AB477425; BAI66653.1; -; mRNA.
DR   RefSeq; NP_001108307.1; NM_001114835.1.
DR   RefSeq; NP_001165362.1; NM_001171891.1.
DR   AlphaFoldDB; D2KUZ7; -.
DR   PRIDE; D2KUZ7; -.
DR   GeneID; 100137708; -.
DR   KEGG; xla:100137708; -.
DR   CTD; 100137708; -.
DR   Xenbase; XB-GENE-1013949; pmepa1.L.
DR   OrthoDB; 1189277at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 100137708; Expressed in internal ear and 19 other tissues.
DR   GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0010008; C:endosome membrane; ISS:UniProtKB.
DR   GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0070412; F:R-SMAD binding; IEA:InterPro.
DR   GO; GO:0060394; P:negative regulation of pathway-restricted SMAD protein phosphorylation; ISS:UniProtKB.
DR   GO; GO:0010991; P:negative regulation of SMAD protein complex assembly; ISS:UniProtKB.
DR   GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; ISS:UniProtKB.
DR   InterPro; IPR039122; TMEPAI.
DR   InterPro; IPR043445; TMEPAI/LRAD4.
DR   PANTHER; PTHR16514; PTHR16514; 1.
DR   PANTHER; PTHR16514:SF5; PTHR16514:SF5; 1.
PE   2: Evidence at transcript level;
KW   Endosome; Golgi apparatus; Membrane; Reference proteome; Repeat;
KW   Signal transduction inhibitor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..285
FT                   /note="Protein TMEPAI"
FT                   /id="PRO_0000430493"
FT   TOPO_DOM        1..38
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        60..285
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          76..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          201..222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          256..285
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           155..158
FT                   /note="PPxY motif 1"
FT   MOTIF           183..186
FT                   /note="SMAD interaction motif (SIM)"
FT   MOTIF           226..229
FT                   /note="PPxY motif 2"
FT   COMPBIAS        270..285
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   285 AA;  32071 MW;  9538E65A6D300CA3 CRC64;
     MHNLMGLNST SESIHSNVSC TCNCKRSLFQ TMEISELEFV QIIIIVVVMM VMVVVITCLL
     NHYKLSARSF IHRHSQGRRR EENLSSEGNL WPSESTVSGN GIIEQHIYTP RPSDRLSVPS
     FLQRDRFNRF QPTYPYIQNE IDLPPTISLS DGEEPPPYQG PCTLQLRDPE QQMELNRESV
     RAPPNRTIFD SDLIDSSVYG GPCPPSSNSG VSATSYSSNG RMEGPPPTYN EVIGHYPRSS
     FYHCQQPNLA TPSILESNRL QPGNHGLDST ITRSKDKDKQ KGQPF