AT12B_ARATH
ID AT12B_ARATH Reviewed; 94 AA.
AC Q9LVK3;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Ubiquitin-like protein ATG12B;
DE AltName: Full=Autophagy-related protein 12b;
DE Short=APG12-like protein b;
DE Short=AtAPG12b;
GN Name=ATG12B; Synonyms=APG12, APG12B; OrderedLocusNames=At3g13970;
GN ORFNames=MDC16.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, AND GENE FAMILY.
RX PubMed=12114572; DOI=10.1104/pp.011024;
RA Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D.,
RA Tabata S., Ohsumi Y.;
RT "Leaf senescence and starvation-induced chlorosis are accelerated by the
RT disruption of an Arabidopsis autophagy gene.";
RL Plant Physiol. 129:1181-1193(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 1-94.
RX PubMed=16874047; DOI=10.4161/auto.1.2.1859;
RA Suzuki N.N., Yoshimoto K., Fujioka Y., Ohsumi Y., Inagaki F.;
RT "The crystal structure of plant ATG12 and its biological implication in
RT autophagy.";
RL Autophagy 1:119-126(2005).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16040659; DOI=10.1104/pp.105.060673;
RA Thompson A.R., Doelling J.H., Suttangkakul A., Vierstra R.D.;
RT "Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and
RT ATG12 conjugation pathways.";
RL Plant Physiol. 138:2097-2110(2005).
CC -!- FUNCTION: Ubiquitin-like protein involved in cytoplasm to vacuole
CC transport (Cvt) and autophagy vesicles formation. Conjugation with ATG5
CC through a ubiquitin-like conjugating system involving also ATG7 as an
CC E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme,
CC is essential for its function. ATG12/ATG5 conjugate has an essential
CC role in plant nutrient recycling. {ECO:0000269|PubMed:16040659}.
CC -!- INTERACTION:
CC Q9LVK3; Q0WWQ1: ATG3; NbExp=2; IntAct=EBI-8276588, EBI-8276607;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16040659}.
CC -!- SIMILARITY: Belongs to the ATG12 family. {ECO:0000305}.
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DR EMBL; AB073185; BAB88397.1; -; mRNA.
DR EMBL; AB019229; BAB02327.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75446.1; -; Genomic_DNA.
DR EMBL; BT024653; ABD57478.1; -; mRNA.
DR EMBL; AK118997; BAC43573.1; -; mRNA.
DR RefSeq; NP_188013.2; NM_112251.4.
DR PDB; 1WZ3; X-ray; 1.80 A; A/B=1-94.
DR PDB; 7EU4; X-ray; 3.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-94.
DR PDBsum; 1WZ3; -.
DR PDBsum; 7EU4; -.
DR AlphaFoldDB; Q9LVK3; -.
DR SMR; Q9LVK3; -.
DR BioGRID; 5945; 1.
DR IntAct; Q9LVK3; 1.
DR MINT; Q9LVK3; -.
DR STRING; 3702.AT3G13970.1; -.
DR PaxDb; Q9LVK3; -.
DR PRIDE; Q9LVK3; -.
DR EnsemblPlants; AT3G13970.1; AT3G13970.1; AT3G13970.
DR GeneID; 820611; -.
DR Gramene; AT3G13970.1; AT3G13970.1; AT3G13970.
DR KEGG; ath:AT3G13970; -.
DR Araport; AT3G13970; -.
DR TAIR; locus:2088182; AT3G13970.
DR eggNOG; KOG3439; Eukaryota.
DR HOGENOM; CLU_106795_3_1_1; -.
DR InParanoid; Q9LVK3; -.
DR OMA; HADNKES; -.
DR OrthoDB; 1525971at2759; -.
DR PhylomeDB; Q9LVK3; -.
DR EvolutionaryTrace; Q9LVK3; -.
DR PRO; PR:Q9LVK3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LVK3; baseline and differential.
DR GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; IBA:GO_Central.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; IGI:TAIR.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0006501; P:C-terminal protein lipidation; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR007242; Atg12.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR13385; PTHR13385; 1.
DR Pfam; PF04110; APG12; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Autophagy; Cytoplasm; Isopeptide bond;
KW Protein transport; Reference proteome; Transport; Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q8S924"
FT CHAIN 2..94
FT /note="Ubiquitin-like protein ATG12B"
FT /id="PRO_0000250543"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q8S924"
FT CROSSLNK 94
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-128 in ATG5)"
FT /evidence="ECO:0000250"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:7EU4"
FT STRAND 7..9
FT /evidence="ECO:0007829|PDB:7EU4"
FT STRAND 11..18
FT /evidence="ECO:0007829|PDB:1WZ3"
FT STRAND 29..33
FT /evidence="ECO:0007829|PDB:1WZ3"
FT HELIX 39..49
FT /evidence="ECO:0007829|PDB:1WZ3"
FT STRAND 55..62
FT /evidence="ECO:0007829|PDB:1WZ3"
FT HELIX 70..77
FT /evidence="ECO:0007829|PDB:1WZ3"
FT STRAND 82..89
FT /evidence="ECO:0007829|PDB:1WZ3"
SQ SEQUENCE 94 AA; 10358 MW; 5BA77795F9C89D6A CRC64;
MATESPNSVQ KIVVHLRATG GAPILKQSKF KVSGSDKFAN VIDFLRRQLH SDSLFVYVNS
AFSPNPDESV IDLYNNFGFD GKLVVNYACS MAWG
