ID   PMFR_PAENI              Reviewed;         470 AA.
AC   Q8GAH9;
DT   11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Transcriptional activator PmfR;
GN   Name=pmfR; ORFNames=ORF67;
OS   Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans).
OG   Plasmid pAO1.
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Paenarthrobacter.
OX   NCBI_TaxID=29320;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 49919 / DSM 420 / JCM 3874 / NBRC 15511; PLASMID=pAO1;
RX   PubMed=12618462; DOI=10.1128/jb.185.6.1976-1986.2003;
RA   Igloi G.L., Brandsch R.;
RT   "Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter
RT   nicotinovorans and identification of a pAO1-dependent nicotine uptake
RT   system.";
RL   J. Bacteriol. 185:1976-1986(2003).
RN   [2]
RP   FUNCTION AS A TRANSCRIPTIONAL ACTIVATOR, DNA-BINDING, GENE NAME, PATHWAY,
RP   SUBUNIT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 49919 / DSM 420 / JCM 3874 / NBRC 15511; PLASMID=pAO1;
RX   PubMed=15838033; DOI=10.1128/jb.187.9.3062-3070.2005;
RA   Chiribau C.B., Sandu C., Igloi G.L., Brandsch R.;
RT   "Characterization of PmfR, the transcriptional activator of the pAO1-borne
RT   purU-mabO-folD operon of Arthrobacter nicotinovorans.";
RL   J. Bacteriol. 187:3062-3070(2005).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 49919 / DSM 420 / JCM 3874 / NBRC 15511; PLASMID=pAO1;
RX   PubMed=17293530; DOI=10.1128/aem.02668-06;
RA   Mihasan M., Chiribau C.B., Friedrich T., Artenie V., Brandsch R.;
RT   "An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon
RT   and may protect Arthrobacter nicotinovorans from oxidative stress during
RT   nicotine catabolism.";
RL   Appl. Environ. Microbiol. 73:2479-2485(2007).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 49919 / DSM 420 / JCM 3874 / NBRC 15511; PLASMID=pAO1;
RX   PubMed=17464069; DOI=10.1099/mic.0.2006/004234-0;
RA   Ganas P., Mihasan M., Igloi G.L., Brandsch R.;
RT   "A two-component small multidrug resistance pump functions as a metabolic
RT   valve during nicotine catabolism by Arthrobacter nicotinovorans.";
RL   Microbiology 153:1546-1555(2007).
CC   -!- FUNCTION: Transcriptional regulator involved in the activation of the
CC       purU-mabO-folD-nepA-nepB and mao-ORF55-nbr operons implicated in the
CC       nicotine catabolic pathway. The sequence GTTT-14 bp-AAAC seems to be
CC       the core binding site of the regulator upstream of the -35 promoter
CC       region of the operon. {ECO:0000269|PubMed:15838033,
CC       ECO:0000269|PubMed:17293530, ECO:0000269|PubMed:17464069}.
CC   -!- PATHWAY: Alkaloid degradation; nicotine degradation [regulation].
CC       {ECO:0000269|PubMed:15838033}.
CC   -!- SUBUNIT: Forms oligomers in solution, probably homotetramers.
CC       {ECO:0000269|PubMed:15838033}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene do not produce the MabO
CC       protein in the presence of nicotine, in contrast to the wild-type.
CC       Transcription of the nepAB genes and of the mao-ORF55-nbr operon is
CC       abolished in the mutant strain. {ECO:0000269|PubMed:15838033,
CC       ECO:0000269|PubMed:17293530, ECO:0000269|PubMed:17464069}.
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DR   EMBL; AJ507836; CAD47925.1; -; Genomic_DNA.
DR   RefSeq; WP_016359436.1; NC_021229.1.
DR   RefSeq; YP_007988751.1; NC_021229.1.
DR   AlphaFoldDB; Q8GAH9; -.
DR   UniPathway; UPA00106; -.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR   GO; GO:0019608; P:nicotine catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   Gene3D; 1.10.10.2840; -; 1.
DR   InterPro; IPR025736; PucR_C-HTH_dom.
DR   InterPro; IPR042070; PucR_C-HTH_sf.
DR   InterPro; IPR012914; PucR_dom.
DR   Pfam; PF13556; HTH_30; 1.
DR   Pfam; PF07905; PucR; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Plasmid; Transcription; Transcription regulation.
FT   CHAIN           1..470
FT                   /note="Transcriptional activator PmfR"
FT                   /id="PRO_0000429451"
SQ   SEQUENCE   470 AA;  51166 MW;  07BE411C0F2EA24A CRC64;
     MEQQVLTVRD LVTAQSLGMK VLSGAGGLDR QVLWAHSCEL SDPDRWLGPH ELLMTVGLCV
     PHSAVEQRNF IVKLDEAGLS GVALGDHNSL PPLTRELYEE ADRRSFPVLL TNQATPFAAI
     GRTVAAATAT TQTMQVLKLS KLYQLSTYAR TDPLRMMNDL QALLRAGLSV FDVQTGLTIV
     EGAPLEFMPT SVRERTYALP GDSDSRLMIS EYPGEEVSSF LLIHVLQVID VALSQLLRSL
     RRRSERSTQM LASIFEGRSP DGLGSILGPS GTSSGYQFVA VALEDSEKVA RAASIKSLPV
     LAGPGSSSFF ILMPEQSRND VRNLLHGLDV RAGVSSTYLD LRDAKAAADE AAKIFSSGGT
     NGLWTDFTGV PVSLLTRSRK EASAIVQQVL GRLAGTDPKI TVLRETLFAF LANDRRWNET
     AAALGIHRQT LSYRLTRIKE ITGRDIASSA DLSAFWLAFQ AWPSFSDRSD