PMI2_ARATH
ID PMI2_ARATH Reviewed; 607 AA.
AC Q9C9N6;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Protein PLASTID MOVEMENT IMPAIRED 2;
DE AltName: Full=Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE LIGHT 2;
DE Short=Protein WEB2;
GN Name=PMI2; Synonyms=WEB2; OrderedLocusNames=At1g66840; ORFNames=F4N21.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16778016; DOI=10.1104/pp.106.080333;
RA Luesse D.R., Deblasio S.L., Hangarter R.P.;
RT "Plastid movement impaired 2, a new gene involved in normal blue-light-
RT induced chloroplast movements in Arabidopsis.";
RL Plant Physiol. 141:1328-1337(2006).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP GENE FAMILY, AND INTERACTION WITH WEB1.
RX PubMed=20974974; DOI=10.1073/pnas.1007836107;
RA Kodama Y., Suetsugu N., Kong S.G., Wada M.;
RT "Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the
RT chloroplast photorelocation movement velocity in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:19591-19596(2010).
CC -!- FUNCTION: Required for the chloroplast avoidance response under high
CC intensity blue light. This avoidance response consists in the
CC relocation of chloroplasts on the anticlinal side of exposed cells.
CC Acts in association with WEB1 to maintain the velocity of chloroplast
CC photorelocation movement via cp-actin filaments regulation.
CC {ECO:0000269|PubMed:16778016, ECO:0000269|PubMed:20974974}.
CC -!- SUBUNIT: Interacts with WEB1. {ECO:0000269|PubMed:20974974}.
CC -!- INTERACTION:
CC Q9C9N6; O48724: WEB1; NbExp=6; IntAct=EBI-4408425, EBI-4408440;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20974974}.
CC -!- TISSUE SPECIFICITY: Ubiquitous but preferentially in chloroplast-
CC containing tissues. {ECO:0000269|PubMed:16778016,
CC ECO:0000269|PubMed:20974974}.
CC -!- DISRUPTION PHENOTYPE: Deficient chloroplast response.
CC {ECO:0000269|PubMed:20974974}.
CC -!- SIMILARITY: Belongs to the WEB family. {ECO:0000305}.
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DR EMBL; AC013288; AAG60086.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34561.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57978.1; -; Genomic_DNA.
DR RefSeq; NP_001320449.1; NM_001334250.1.
DR RefSeq; NP_176856.1; NM_105355.3.
DR AlphaFoldDB; Q9C9N6; -.
DR SMR; Q9C9N6; -.
DR IntAct; Q9C9N6; 1.
DR STRING; 3702.AT1G66840.1; -.
DR iPTMnet; Q9C9N6; -.
DR PaxDb; Q9C9N6; -.
DR PRIDE; Q9C9N6; -.
DR ProteomicsDB; 234738; -.
DR EnsemblPlants; AT1G66840.1; AT1G66840.1; AT1G66840.
DR EnsemblPlants; AT1G66840.2; AT1G66840.2; AT1G66840.
DR GeneID; 843002; -.
DR Gramene; AT1G66840.1; AT1G66840.1; AT1G66840.
DR Gramene; AT1G66840.2; AT1G66840.2; AT1G66840.
DR KEGG; ath:AT1G66840; -.
DR Araport; AT1G66840; -.
DR TAIR; locus:2033399; AT1G66840.
DR eggNOG; ENOG502QR0X; Eukaryota.
DR HOGENOM; CLU_017212_1_1_1; -.
DR InParanoid; Q9C9N6; -.
DR OMA; GSFQFMT; -.
DR OrthoDB; 1126798at2759; -.
DR PhylomeDB; Q9C9N6; -.
DR PRO; PR:Q9C9N6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9N6; baseline and differential.
DR Genevisible; Q9C9N6; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0009904; P:chloroplast accumulation movement; IMP:TAIR.
DR GO; GO:0009903; P:chloroplast avoidance movement; IMP:TAIR.
DR GO; GO:0009637; P:response to blue light; IMP:TAIR.
DR InterPro; IPR008545; Web.
DR Pfam; PF05701; WEMBL; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Reference proteome.
FT CHAIN 1..607
FT /note="Protein PLASTID MOVEMENT IMPAIRED 2"
FT /id="PRO_0000283627"
FT COILED 66..295
FT /evidence="ECO:0000255"
FT COILED 329..445
FT /evidence="ECO:0000255"
SQ SEQUENCE 607 AA; 70240 MW; 3BCC9D4188A87158 CRC64;
MGERNLDGTV SVKATINKYG QKATRSVIKS SVAEDLHKSG RELGIYRESR RVAESAKAKA
EVELCKAKKI VKELTLRIEE SNRRLKSRRI DIEAVMNESR IDGNGGYVRI MRELEDMKQE
LSKLKLDVVY VSREKVVAEK EVMELESRME ENLKLLESLK LEVDVANEEH VLVEVAKIEA
LKECKEVEEQ REKERKEVSE SLHKRKKRIR EMIREIERSK NFENELAETL LDIEMLETQL
KLVKEMERKV QRNESMSRSK NRAFERGKDN LSVLKEVTEA TEAKKAELAS INAELFCLVN
TMDTLRKEFD HAKKETAWLD KMIQKDDVML ERLNTKLLIA KDQLEAVSKA EERISYLADN
LTTSFEKLKS DREAAKKEEL KLREEARIIN NEIQKTETGF DGKEKELLSK LDELEKAKHA
ESLALEKLET MVEKTMETRE MESRRNSTIT ISRFEYEYLS GKACHAEETA EKKVEAAMAW
VEALKASTKA IMIKTESLKR VSGKTMLEEE RESFRMQRSL SIKRLVQDEI QKFKGNSEDN
GLINSPKPVR KSVRLSGKFA PVQGGKSRRY SSGNRATPTF FVIKKKKKVP NMVKFFSRKR
RNSSLEQ
