PMIP2_CRYNJ
ID PMIP2_CRYNJ Reviewed; 823 AA.
AC P0CQ20; Q55XK7; Q5KMC8;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Mitochondrial intermediate peptidase 2;
DE Short=MIP 2;
DE EC=3.4.24.59;
DE AltName: Full=Octapeptidyl aminopeptidase 2;
DE Flags: Precursor;
GN Name=OCT2; OrderedLocusNames=CNB02140;
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Cleaves proteins, imported into the mitochondrion, to their
CC mature size. While most mitochondrial precursor proteins are processed
CC to the mature form in one step by mitochondrial processing peptidase
CC (MPP), the sequential cleavage by MIP of an octapeptide after initial
CC processing by MPP is a required step for a subgroup of nuclear-encoded
CC precursor proteins destined for the matrix or the inner membrane (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal octapeptide as second stage of
CC processing of some proteins imported into the mitochondrion.;
CC EC=3.4.24.59;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase M3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAW41837.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE017342; AAW41837.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_569144.1; XM_569144.1.
DR AlphaFoldDB; P0CQ20; -.
DR SMR; P0CQ20; -.
DR STRING; 5207.AAW41837; -.
DR PaxDb; P0CQ20; -.
DR EnsemblFungi; AAW41837; AAW41837; CNB02140.
DR GeneID; 3255573; -.
DR KEGG; cne:CNB02140; -.
DR VEuPathDB; FungiDB:CNB02140; -.
DR eggNOG; KOG2090; Eukaryota.
DR InParanoid; P0CQ20; -.
DR OrthoDB; 642479at2759; -.
DR Proteomes; UP000002149; Chromosome 2.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR GO; GO:0006518; P:peptide metabolic process; IBA:GO_Central.
DR GO; GO:0006627; P:protein processing involved in protein targeting to mitochondrion; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR Gene3D; 1.10.1370.10; -; 1.
DR Gene3D; 3.40.390.10; -; 1.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR024077; Neurolysin/TOP_dom2.
DR InterPro; IPR045090; Pept_M3A_M3B.
DR InterPro; IPR001567; Pept_M3A_M3B_dom.
DR PANTHER; PTHR11804; PTHR11804; 1.
DR Pfam; PF01432; Peptidase_M3; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 3: Inferred from homology;
KW Hydrolase; Metal-binding; Metalloprotease; Mitochondrion; Protease;
KW Reference proteome; Transit peptide; Zinc.
FT TRANSIT 1..33
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 34..823
FT /note="Mitochondrial intermediate peptidase 2"
FT /id="PRO_0000338582"
FT REGION 532..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 596
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 595
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 599
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 602
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ SEQUENCE 823 AA; 92172 MW; 4C30FAD763938997 CRC64;
MRRLQQSLRR RSARRCPFIL IPHRLLTTSY ASYKPAPQAT LEIEDTNSPT FLLKTSPIQL
PARATSDDSA IKAHFDLPHS IFGDMVGVRR EHVKGLFHYD SLTQPESLMR LTDRTLIQAS
AIVQRIVVAP QDPTGRELRL VVKNLDRLSD ILCGVIDMCE LIRNVHPHQD WVNQSDRTHQ
ILCSFMNELN ATRGLYESLA KAIAHPFNDP LTTSELRVAR IFLTDFERSG IHLPPSVRER
FVKHSDALLF LGRSFLSSAS SGPSTVPHIE IPDPHRLLTG LGRQFVDSLP RTGRNGQAVI
EPGSWEAQMI LRYAREGRAR ELVYVGGMRA DKKRISVLEA MLKERAELAS VLGKNNWAEV
VLVDKMTKTP ENVMRFLTSL AQHHQPVARA EVDMLRRMKA TALTGNYFDP RNSRTRHLPL
FHAWDRDYYS DKYLTSLIPT GSPPSISPYL STGTVMSGLS RIFSRLYGIS FKPAVVSPGE
VWHPSVRRLD VVHEEEGLIG VIYCDFFSRI GKSSGAAHYT VRCSRRVDDD DIDGDGLPED
WDKPYGPGLE ADKESLSGKP GKYQLPIIAL SMDVGTVNEG RPALLNWQEL ETLFHEMGHA
IHSMIGRTEY HNVSGTRCAT DFVELPSILM EHFVSSPEVL STLAFHHATG EPLPIPVIEA
HLALNQSLSA LETHGQIAMA LLDQKYHTLR HGQDSFDSTA IWFQLQQEIG VIQPVPGTAW
QMQFGHLYGY GATYYSYLFD RAIAGKIWST LFHRSGTSQA YDRKAEGILS REGGELLKEK
VLKWGGGRDP WEMVGDVIGG VEGDELSKGD ERALALVGSW SVV