ID   PMIP_KLULA              Reviewed;         779 AA.
AC   Q6CVF7;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Mitochondrial intermediate peptidase;
DE            Short=MIP;
DE            EC=3.4.24.59;
DE   AltName: Full=Octapeptidyl aminopeptidase;
DE   Flags: Precursor;
GN   Name=OCT1; OrderedLocusNames=KLLA0B12397g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Cleaves proteins, imported into the mitochondrion, to their
CC       mature size. While most mitochondrial precursor proteins are processed
CC       to the mature form in one step by mitochondrial processing peptidase
CC       (MPP), the sequential cleavage by MIP of an octapeptide after initial
CC       processing by MPP is a required step for a subgroup of nuclear-encoded
CC       precursor proteins destined for the matrix or the inner membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Release of an N-terminal octapeptide as second stage of
CC         processing of some proteins imported into the mitochondrion.;
CC         EC=3.4.24.59;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M3 family. {ECO:0000305}.
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DR   EMBL; CR382122; CAH02475.1; -; Genomic_DNA.
DR   RefSeq; XP_452082.1; XM_452082.1.
DR   AlphaFoldDB; Q6CVF7; -.
DR   SMR; Q6CVF7; -.
DR   STRING; 28985.XP_452082.1; -.
DR   MEROPS; M03.006; -.
DR   EnsemblFungi; CAH02475; CAH02475; KLLA0_B12397g.
DR   GeneID; 2897324; -.
DR   KEGG; kla:KLLA0_B12397g; -.
DR   eggNOG; KOG2090; Eukaryota.
DR   HOGENOM; CLU_001805_0_0_1; -.
DR   InParanoid; Q6CVF7; -.
DR   OMA; VVYCDLF; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IEA:EnsemblFungi.
DR   GO; GO:0006627; P:protein processing involved in protein targeting to mitochondrion; IEA:EnsemblFungi.
DR   GO; GO:0050821; P:protein stabilization; IEA:EnsemblFungi.
DR   Gene3D; 1.10.1370.10; -; 1.
DR   Gene3D; 3.40.390.10; -; 1.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR024077; Neurolysin/TOP_dom2.
DR   InterPro; IPR045090; Pept_M3A_M3B.
DR   InterPro; IPR001567; Pept_M3A_M3B_dom.
DR   PANTHER; PTHR11804; PTHR11804; 1.
DR   Pfam; PF01432; Peptidase_M3; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Mitochondrion; Protease;
KW   Reference proteome; Transit peptide; Zinc.
FT   TRANSIT         1..27
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..779
FT                   /note="Mitochondrial intermediate peptidase"
FT                   /id="PRO_0000338584"
FT   ACT_SITE        566
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         565
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         569
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         572
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   779 AA;  89380 MW;  E25823250E5683BF CRC64;
     MLRTVSVGRQ YVQRYFGHCL IVNSVRHSSS ATVNRLKQPL RRVFDDDSHW RALNHSNYKL
     NESKGRKFGL RSTSDLETGL FQNSYLKSAN GLVEFTQHSF EKAKELVQKI HSIETQDEMK
     YYIKDLDQLS DVLCRVIDLC EFIRATHPDK KFVQTAQQCH EKMFEIMNIL NTDVRLCDLL
     TQCLRESDVL GLDSEEIRTG KILLEDFEKS GIYMKPEIRE KFIQLSQEIS VIGQDFINNT
     EYVRSNYIKI SCELMDAHVN KMVCSQMKKD ITGEYYKVPT YGYIPHTLLR TCSDEVIRMK
     IWTEMHSCSD AQIERLTKLI SLRVELAKLL GSQNFAQYQL HGKMAKTPEN VSGFLESLVH
     STRIKAASEL KPLAVLKSEL TGTQTPHTSE EVLELMKPWD RDYYGSIQAL AQRRSSSLDN
     GESISSSFSL GVVMQGLSDL FEKIYGIKLV PATPKTGETW SPDVRRIDVV DEHDGLIGVM
     YCDLFEREGK TPNPAHFTVC CSRNMYLNEA DTSTIQVGVN SNGQKFQLPV ISLVCDFRWV
     EVNMGDGKHQ QMCLLQLNEI ETLFHEMGHA MHSMLGRTQL QNVSGTRCAT DFVELPSILM
     EHFARDTRVL SSISSHYKTG KSLDVEVLKN HQLENQFLQN CETFSQIKMS FLDQELHNLD
     HTTDGSIDVI AIYHRLERRL AVLPDDQSNW CGKFGHLFGY GASYYSYLFD RAIASKIWDH
     LFEQDPFNRT NGTKFKEGLL QWGGSRDPWY LLSQVLDEPR LAKGDEWGMR YIGDVKTGM