PML1_YEAST
ID PML1_YEAST Reviewed; 204 AA.
AC Q07930; D6VY18; Q06901;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Pre-mRNA leakage protein 1;
GN Name=PML1; OrderedLocusNames=YLR016C; ORFNames=L1591;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RA Saville S.P., Atkinson S., Jamieson L., Pocklington M.J., Orr E.;
RT "A 7.8kb fragment from chromosome XII of Saccharomyces cerevisiae does not
RT harbour PKC2.";
RL Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH IST3
RP AND BUD13.
RX PubMed=15565172; DOI=10.1038/sj.emboj.7600482;
RA Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F.,
RA Laprevote O., Seraphin B.;
RT "Proteomic analysis identifies a new complex required for nuclear pre-mRNA
RT retention and splicing.";
RL EMBO J. 23:4847-4856(2004).
CC -!- FUNCTION: Required for efficient splicing and pre-mRNA nuclear
CC retention. {ECO:0000269|PubMed:15565172}.
CC -!- SUBUNIT: Belongs to the pre-mRNA retention and splicing (RES) complex
CC composed of at least BUD13, IST3 and PML1.
CC -!- INTERACTION:
CC Q07930; P40565: IST3; NbExp=5; IntAct=EBI-27110, EBI-25387;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1470 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA62157.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X90564; CAA62157.1; ALT_FRAME; Genomic_DNA.
DR EMBL; Z73188; CAA97538.1; -; Genomic_DNA.
DR EMBL; AY557934; AAS56260.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09334.1; -; Genomic_DNA.
DR PIR; S64838; S64838.
DR RefSeq; NP_013116.1; NM_001181903.1.
DR PDB; 2JKD; X-ray; 2.50 A; A/B=25-204.
DR PDB; 2MKC; NMR; -; B=22-42.
DR PDB; 2MY3; NMR; -; B=22-42.
DR PDB; 3ELS; X-ray; 1.80 A; A=51-204.
DR PDB; 3ELV; X-ray; 2.40 A; A/B=1-204.
DR PDB; 5GM6; EM; 3.50 A; U=1-204.
DR PDB; 5LQW; EM; 5.80 A; N=1-204.
DR PDB; 5ZWM; EM; 3.40 A; Z=1-204.
DR PDB; 5ZWO; EM; 3.90 A; Z=1-204.
DR PDB; 7DCO; EM; 2.50 A; Y=1-204.
DR PDBsum; 2JKD; -.
DR PDBsum; 2MKC; -.
DR PDBsum; 2MY3; -.
DR PDBsum; 3ELS; -.
DR PDBsum; 3ELV; -.
DR PDBsum; 5GM6; -.
DR PDBsum; 5LQW; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR PDBsum; 7DCO; -.
DR AlphaFoldDB; Q07930; -.
DR BMRB; Q07930; -.
DR SMR; Q07930; -.
DR BioGRID; 31290; 112.
DR ComplexPortal; CPX-1649; RES complex.
DR ComplexPortal; CPX-26; U2 small nuclear ribonucleoprotein complex.
DR DIP; DIP-2149N; -.
DR IntAct; Q07930; 10.
DR STRING; 4932.YLR016C; -.
DR MaxQB; Q07930; -.
DR PaxDb; Q07930; -.
DR PRIDE; Q07930; -.
DR EnsemblFungi; YLR016C_mRNA; YLR016C; YLR016C.
DR GeneID; 850703; -.
DR KEGG; sce:YLR016C; -.
DR SGD; S000004006; PML1.
DR VEuPathDB; FungiDB:YLR016C; -.
DR eggNOG; KOG1882; Eukaryota.
DR GeneTree; ENSGT00940000156553; -.
DR HOGENOM; CLU_022457_1_3_1; -.
DR InParanoid; Q07930; -.
DR OMA; NGRSCYL; -.
DR BioCyc; YEAST:G3O-32177-MON; -.
DR EvolutionaryTrace; Q07930; -.
DR PRO; PR:Q07930; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q07930; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0070274; C:RES complex; IDA:SGD.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005686; C:U2 snRNP; IC:ComplexPortal.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0051237; P:maintenance of RNA location; IMP:SGD.
DR GO; GO:0006406; P:mRNA export from nucleus; IMP:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IDA:SGD.
DR GO; GO:1903241; P:U2-type prespliceosome assembly; IC:ComplexPortal.
DR CDD; cd00060; FHA; 1.
DR DisProt; DP01890; -.
DR InterPro; IPR000253; FHA_dom.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR Pfam; PF00498; FHA; 1.
DR SMART; SM00240; FHA; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR PROSITE; PS50006; FHA_DOMAIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome.
FT CHAIN 1..204
FT /note="Pre-mRNA leakage protein 1"
FT /id="PRO_0000058466"
FT DOMAIN 104..172
FT /note="FHA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT CONFLICT 15
FT /note="G -> A (in Ref. 1; CAA62157)"
FT /evidence="ECO:0000305"
FT HELIX 38..41
FT /evidence="ECO:0007829|PDB:2MKC"
FT HELIX 61..67
FT /evidence="ECO:0007829|PDB:3ELS"
FT HELIX 72..74
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 78..84
FT /evidence="ECO:0007829|PDB:3ELS"
FT HELIX 85..90
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 93..97
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 102..108
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 128..131
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 141..148
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 151..158
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 178..180
FT /evidence="ECO:0007829|PDB:2JKD"
FT STRAND 184..190
FT /evidence="ECO:0007829|PDB:3ELS"
FT HELIX 192..194
FT /evidence="ECO:0007829|PDB:3ELS"
FT STRAND 197..203
FT /evidence="ECO:0007829|PDB:3ELS"
SQ SEQUENCE 204 AA; 23654 MW; DA39B4465EFFD378 CRC64;
MFHRRKRPYN TRNYGHDDKK FKSQYIDIMP DFSPSGLLEL ESNNKEGIAL KHVEPQDAIS
PDNYMDMLGL EARDRTMYEL VIYRKNDKDK GPWKRYDLNG RSCYLVGREL GHSLDTDLDD
RTEIVVADIG IPEETSSKQH CVIQFRNVRG ILKCYVMDLD SSNGTCLNNV VIPGARYIEL
RSGDVLTLSE FEEDNDYELI FMNV
