ID   PMN14_PINFU             Reviewed;         121 AA.
AC   Q6F4C6;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Prismalin-14;
DE   Flags: Precursor;
OS   Pinctada fucata (Akoya pearl oyster) (Pinctada imbricata fucata).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX   NCBI_TaxID=50426;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAD27406.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-121, PYROGLUTAMATE
RP   FORMATION AT GLN-17, FUNCTION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Shell {ECO:0000269|PubMed:15132736};
RX   PubMed=15132736; DOI=10.1042/bj20040319;
RA   Suzuki M., Murayama E., Inoue H., Ozaki N., Tohse H., Kogure T.,
RA   Nagasawa H.;
RT   "Characterization of Prismalin-14, a novel matrix protein from the
RT   prismatic layer of the Japanese pearl oyster (Pinctada fucata).";
RL   Biochem. J. 382:205-213(2004).
CC   -!- FUNCTION: Displays inhibitory activity against calcium carbonate
CC       precipitation, binds calcium and affects crystallization of calcium
CC       carbonate in vitro. May be involved in calcification of the prismatic
CC       layer of the shell. {ECO:0000269|PubMed:15132736,
CC       ECO:0000303|PubMed:15132736}.
CC   -!- TISSUE SPECIFICITY: Expressed only at the mantle edge where it is found
CC       predominantly in the inner side of the outer mantle fold.
CC       {ECO:0000269|PubMed:15132736}.
CC   -!- MASS SPECTROMETRY: Mass=11890.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15132736};
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DR   EMBL; AB159512; BAD27406.1; -; mRNA.
DR   AlphaFoldDB; Q6F4C6; -.
DR   GO; GO:0005509; F:calcium ion binding; IDA:UniProtKB.
DR   GO; GO:0031215; P:shell calcification; NAS:UniProtKB.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Pyrrolidone carboxylic acid; Repeat;
KW   Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000269|PubMed:15132736"
FT   CHAIN           17..121
FT                   /note="Prismalin-14"
FT                   /evidence="ECO:0000269|PubMed:15132736"
FT                   /id="PRO_0000022070"
FT   REPEAT          48..51
FT                   /note="1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          52..55
FT                   /note="2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          56..59
FT                   /note="3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          60..63
FT                   /note="4"
FT                   /evidence="ECO:0000255"
FT   REGION          48..63
FT                   /note="4 X 4 AA approximate tandem repeats of P-I-Y-R"
FT   MOD_RES         17
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000303|PubMed:15132736"
SQ   SEQUENCE   121 AA;  13491 MW;  14E4407ACA20B25D CRC64;
     MRSLLVLLAL AACASAQYFF RGGDDDNGFF GGDDDNGYFG YFPRFSYPIY RPIYRPIYYP
     QIIRPFYGYG YGGFNGGYGG LGLYGGYGGF GGYGYRPFSY GYNPFSYGYY GFGDDDGGFD
     D