AT16_ACTCC
ID AT16_ACTCC Reviewed; 456 AA.
AC A0A2R6Q326;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 02-DEC-2020, sequence version 2.
DT 03-AUG-2022, entry version 14.
DE RecName: Full=Alcohol acyltransferase 16 {ECO:0000303|Ref.2};
DE Short=AcAT16 {ECO:0000303|Ref.2};
DE EC=2.3.1.- {ECO:0000269|PubMed:21450321};
GN Name=AT16 {ECO:0000303|Ref.2};
GN ORFNames=CEY00_Acc22632 {ECO:0000312|EMBL:PSS01274.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5; TISSUE=Leaf;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fruit;
RA Beuning L., Bowen J., Crowhurst R., Gleave A., Janssen B., McArtney S.,
RA Newcomb R., Ross G., Snowden K., Walton E., Yauk Y.;
RT "Plant and food research apple EST project.";
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND
RP INDUCTION BY ETHYLENE.
RX PubMed=21450321; DOI=10.1016/j.phytochem.2011.02.026;
RA Guenther C.S., Chervin C., Marsh K.B., Newcomb R.D., Souleyre E.J.F.;
RT "Characterisation of two alcohol acyltransferases from kiwifruit (Actinidia
RT spp.) reveals distinct substrate preferences.";
RL Phytochemistry 72:700-710(2011).
CC -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC kiwifruit flavor (PubMed:21450321). Alcohol acyl transferase that can
CC use a wide range of alcohols as substrate to produce esters
CC (PubMed:21450321). Exhibits benzoyl-CoA:alcohol O-acyltransferase
CC activity (PubMed:21450321). {ECO:0000269|PubMed:21450321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(methylsulfanyl)propanoyl-CoA + butan-1-ol = butyl 3-
CC (methylsulfanyl)propanoate + CoA; Xref=Rhea:RHEA:64676,
CC ChEBI:CHEBI:28885, ChEBI:CHEBI:57287, ChEBI:CHEBI:82815,
CC ChEBI:CHEBI:156073; Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64677;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=benzoyl-CoA + ethanol = CoA + ethyl benzoate;
CC Xref=Rhea:RHEA:64680, ChEBI:CHEBI:16236, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57369, ChEBI:CHEBI:156074;
CC Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64681;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=benzoyl-CoA + butan-1-ol = butyl benzoate + CoA;
CC Xref=Rhea:RHEA:64636, ChEBI:CHEBI:28885, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57369, ChEBI:CHEBI:156070;
CC Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64637;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(methylsulfanyl)acetyl-CoA + butan-1-ol = butyl 2-
CC (methylsulfanyl)acetate + CoA; Xref=Rhea:RHEA:64672,
CC ChEBI:CHEBI:28885, ChEBI:CHEBI:57287, ChEBI:CHEBI:156076,
CC ChEBI:CHEBI:156077; Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64673;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- TISSUE SPECIFICITY: Expressed in fruit. {ECO:0000269|PubMed:21450321}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in kiwifruit during ripening.
CC {ECO:0000269|PubMed:21450321}.
CC -!- INDUCTION: Induced by ethylene in ripe fruits.
CC {ECO:0000269|PubMed:21450321}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=PSS01274.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; NKQK01000020; PSS01274.1; ALT_SEQ; Genomic_DNA.
DR EMBL; HO772640; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; A0A2R6Q326; -.
DR SMR; A0A2R6Q326; -.
DR STRING; 1590841.A0A2R6Q326; -.
DR EnsemblPlants; PSS01274; PSS01274; CEY00_Acc22632.
DR Gramene; PSS01274; PSS01274; CEY00_Acc22632.
DR Proteomes; UP000241394; Chromosome lg20.
DR GO; GO:0016746; F:acyltransferase activity; IDA:UniProtKB.
DR GO; GO:0006066; P:alcohol metabolic process; IDA:UniProtKB.
DR GO; GO:0009836; P:fruit ripening, climacteric; IEP:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..456
FT /note="Alcohol acyltransferase 16"
FT /id="PRO_0000451705"
FT ACT_SITE 167
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT ACT_SITE 382
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
SQ SEQUENCE 456 AA; 51005 MW; A20058457F0E4589 CRC64;
MASFPPSLVF TVRRNEPTLV LPSKSTPREL KQLSDIDDQE GLRFQVPVIM FYKRKLSMEG
EDPVKVIREA LAEALVFYYP FAGRLIEGPN RKLMVDCTSE GVLFIEADAD IEVNQLIGDT
IDPGFSYLDE LLHDVPGSEG ILGCPLLLIQ VTRFRCGGWA FAIRLNHTMS DAPGLVQLLT
TIAEFARGAE GAPSVPPVWQ REFLAARQPP SITFQHHEYE QVINTTTDDN KSMTHKSFFF
GPKEIRAIRS HFPPHYRSVS STFDVLTACL WRCRTCALGL DPPKTVRISC AANGRGKHDL
HVPRGYYGNV FAFPAVVSRA GMISTSSLEY TVEEVKKAKA RMTGEYLRSV ADLMVTKGRP
LYTVAGNYIV SDTTRVGFDA IDFGWGKPVY GGPARAFPLI SFYARFKNNR GEDGTVVLIC
LPEAAMKRFQ DELKKMTEEH VDGPFEYKLI KVMSKL
