PMPM_PSEAE
ID PMPM_PSEAE Reviewed; 477 AA.
AC Q9I3Y3;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Multidrug resistance protein PmpM;
DE AltName: Full=H(+)/drug antiporter;
DE AltName: Full=Multidrug-efflux transporter;
GN Name=pmpM; Synonyms=norM; OrderedLocusNames=PA1361;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=14679249; DOI=10.1128/jb.186.1.262-265.2004;
RA He G.-X., Kuroda T., Mima T., Morita Y., Mizushima T., Tsuchiya T.;
RT "An H(+)-coupled multidrug efflux pump, PmpM, a member of the MATE family
RT of transporters, from Pseudomonas aeruginosa.";
RL J. Bacteriol. 186:262-265(2004).
CC -!- FUNCTION: Multidrug efflux pump that functions as an H(+)/drug
CC antiporter. Confers resistance to benzalkonium chloride,
CC fluoroquinolones, ethidium bromide, acriflavine and
CC tetraphenylphosphonium chloride. {ECO:0000269|PubMed:14679249}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC 2.A.66.1) family. {ECO:0000305}.
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DR EMBL; AE004091; AAG04750.1; -; Genomic_DNA.
DR PIR; B83476; B83476.
DR RefSeq; NP_250052.1; NC_002516.2.
DR RefSeq; WP_003112388.1; NZ_QZGE01000005.1.
DR AlphaFoldDB; Q9I3Y3; -.
DR SMR; Q9I3Y3; -.
DR STRING; 287.DR97_434; -.
DR TCDB; 2.A.66.1.12; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR PaxDb; Q9I3Y3; -.
DR PRIDE; Q9I3Y3; -.
DR EnsemblBacteria; AAG04750; AAG04750; PA1361.
DR GeneID; 880959; -.
DR KEGG; pae:PA1361; -.
DR PATRIC; fig|208964.12.peg.1414; -.
DR PseudoCAP; PA1361; -.
DR HOGENOM; CLU_012893_6_0_6; -.
DR InParanoid; Q9I3Y3; -.
DR OMA; TGNQKVG; -.
DR PhylomeDB; Q9I3Y3; -.
DR BioCyc; PAER208964:G1FZ6-1387-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0098567; C:periplasmic side of plasma membrane; IDA:PseudoCAP.
DR GO; GO:0005886; C:plasma membrane; IDA:PseudoCAP.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IBA:GO_Central.
DR InterPro; IPR002528; MATE_fam.
DR Pfam; PF01554; MatE; 2.
DR PIRSF; PIRSF006603; DinF; 1.
DR TIGRFAMs; TIGR00797; matE; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Antiport; Cell inner membrane; Cell membrane;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..477
FT /note="Multidrug resistance protein PmpM"
FT /id="PRO_0000164231"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 477 AA; 50870 MW; D83E1754579BD568 CRC64;
MNSPALPLSR GLRIRAELKE LLTLAAPIMI AQLATTAMGF VDAVMAGRAS PHDLAAVALG
NSIWIPMFLL MTGTLLATTA KVAQRHGAGD QPGTGPLVRQ ALWLALLIGP LSGAVLWWLS
EPILGLMKVR PELIGPSLLY LKGIALGFPA AALYHVLRCY TNGLGRTRPS MVLGIGGLLL
NIPINYALIY GHFGMPKMGG PGCGWATGSV MWFMFLGMLF WVNKASIYRA SQLFSRWEWP
DRATIGPLVA VGLPIGIAVF AESSIFSVIA LLIGGLDENV VAGHQIALNF SALVFMIPYS
LGMAVTVRVG HNLGAGLPRD ARFAAGVGMA AALGYACVSA SLMLLLREQI AAMYSPDPAV
IAIAASLIVF SALFQFSDAL QVTAAGALRG YQDTRVTMIM TLFAYWGIGL PVGYSLGLTD
WFQEPTGPRG LWQGLVVGLT GAAIMLCIRL ARSARRFIRQ HERLQREDAE AASVLGR
