PMRA_PSEAE
ID PMRA_PSEAE Reviewed; 221 AA.
AC Q9HV32;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Response regulator protein PmrA {ECO:0000303|PubMed:14507375};
GN Name=pmrA; OrderedLocusNames=PA4776;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, AND INDUCTION BY CATIONIC ANTIMICROBIAL PEPTIDES.
RX PubMed=14507375; DOI=10.1046/j.1365-2958.2003.03673.x;
RA McPhee J.B., Lewenza S., Hancock R.E.;
RT "Cationic antimicrobial peptides activate a two-component regulatory
RT system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic
RT antimicrobial peptides in Pseudomonas aeruginosa.";
RL Mol. Microbiol. 50:205-217(2003).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RX PubMed=16707691; DOI=10.1128/jb.00053-06;
RA McPhee J.B., Bains M., Winsor G., Lewenza S., Kwasnicka A., Brazas M.D.,
RA Brinkman F.S., Hancock R.E.;
RT "Contribution of the PhoP-PhoQ and PmrA-PmrB two-component regulatory
RT systems to Mg2+-induced gene regulation in Pseudomonas aeruginosa.";
RL J. Bacteriol. 188:3995-4006(2006).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=29379484; DOI=10.3389/fmicb.2017.02690;
RA Liu W., Li M., Jiao L., Wang P., Yan Y.;
RT "PmrA/PmrB Two-Component System Regulation of lipA Expression in
RT Pseudomonas aeruginosa PAO1.";
RL Front. Microbiol. 8:2690-2690(2017).
CC -!- FUNCTION: Member of the two-component regulatory system PmrA/PmrB that
CC plays a role in the regulation of resistance towards polymyxin B and
CC cationic antimicrobial peptides in response to limiting concentrations
CC of Mg(2+) (PubMed:14507375). Functions as a transcriptional activator
CC by direct binding to a cis-acting sequence upstream of the target gene
CC promoters including lipase lipA and pmrH promoters (PubMed:16707691,
CC PubMed:29379484). Autoregulates also its own pmrAB operon under Mg(2+)-
CC limiting conditions (PubMed:14507375, PubMed:16707691).
CC {ECO:0000269|PubMed:14507375, ECO:0000269|PubMed:16707691,
CC ECO:0000269|PubMed:29379484}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By cationic antimicrobial peptides and by Mg(2+)-limiting
CC conditions. {ECO:0000269|PubMed:14507375}.
CC -!- DISRUPTION PHENOTYPE: Deletion leads to transcriptional alteration of
CC multiple genes including a large number of genes involved in metal
CC transport, such as the feoAB operon involved in ferrous iron uptake and
CC the mgtE and mgtA operons involved in Mg(2+) transport.
CC {ECO:0000269|PubMed:16707691}.
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DR EMBL; AE004091; AAG08162.1; -; Genomic_DNA.
DR PIR; F83048; F83048.
DR RefSeq; NP_253464.1; NC_002516.2.
DR RefSeq; WP_003102236.1; NZ_QZGE01000018.1.
DR AlphaFoldDB; Q9HV32; -.
DR SMR; Q9HV32; -.
DR STRING; 287.DR97_2120; -.
DR PaxDb; Q9HV32; -.
DR PRIDE; Q9HV32; -.
DR EnsemblBacteria; AAG08162; AAG08162; PA4776.
DR GeneID; 881834; -.
DR KEGG; pae:PA4776; -.
DR PATRIC; fig|208964.12.peg.5003; -.
DR PseudoCAP; PA4776; -.
DR HOGENOM; CLU_000445_30_1_6; -.
DR InParanoid; Q9HV32; -.
DR OMA; YFEGANT; -.
DR PhylomeDB; Q9HV32; -.
DR BioCyc; PAER208964:G1FZ6-4889-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IDA:PseudoCAP.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..221
FT /note="Response regulator protein PmrA"
FT /id="PRO_0000449418"
FT DOMAIN 2..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 124..218
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 51
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 221 AA; 24394 MW; ADFB426D89B7E356 CRC64;
MRILLAEDDL LLGDGIRAGL RLEGDTVEWV TDGVAAENAL VTDEFDLLVL DIGLPRRSGL
DILRNLRHQG LLTPVLLLTA RDKVADRVAG LDSGADDYLT KPFDLDELQA RVRALTRRTT
GRALPQLVHG ELRLDPATHQ VTLSGQAVEL APREYALLRL LLENSGKVLS RNQLEQSLYG
WSGDVESNAI EVHVHHLRRK LGNQLIRTVR GIGYGIDQPA P
