ID   PMRA_STRPN              Reviewed;         399 AA.
AC   P0A4K4; Q9ZEX9;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Multi-drug resistance efflux pump PmrA;
GN   Name=pmrA; OrderedLocusNames=SP_0972;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9869592; DOI=10.1128/aac.43.1.187;
RA   Gill M.J., Brenwald N.P., Wise R.;
RT   "Identification of an efflux pump gene, pmrA, associated with
RT   fluoroquinolone resistance in Streptococcus pneumoniae.";
RL   Antimicrob. Agents Chemother. 43:187-189(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- FUNCTION: Efflux pump for various substrates.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; AJ007367; CAA07482.1; -; Genomic_DNA.
DR   EMBL; AE005672; AAK75093.1; -; Genomic_DNA.
DR   PIR; D95112; D95112.
DR   RefSeq; WP_000136147.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; P0A4K4; -.
DR   SMR; P0A4K4; -.
DR   STRING; 170187.SP_0972; -.
DR   TCDB; 2.A.1.2.34; the major facilitator superfamily (mfs).
DR   EnsemblBacteria; AAK75093; AAK75093; SP_0972.
DR   GeneID; 60234173; -.
DR   KEGG; spn:SP_0972; -.
DR   eggNOG; COG2814; Bacteria.
DR   OMA; IVAPLWG; -.
DR   PhylomeDB; P0A4K4; -.
DR   BioCyc; SPNE170187:G1FZB-1000-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR005828; MFS_sugar_transport-like.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR   Pfam; PF07690; MFS_1; 1.
DR   Pfam; PF00083; Sugar_tr; 1.
DR   PRINTS; PR01035; TCRTETA.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..399
FT                   /note="Multi-drug resistance efflux pump PmrA"
FT                   /id="PRO_0000173377"
FT   TRANSMEM        12..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        49..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        248..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        340..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        366..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   399 AA;  42840 MW;  58F97634E006AD8E CRC64;
     MTEINWKDNL RIAWFGNFLT GASISLVVPF MPIFVENLGV GSQQVAFYAG LAISVSAISA
     ALFSPIWGIL ADKYGRKPMM IRAGLAMTIT MGGLAFVPNI YWLIFLRLLN GVFAGFVPNA
     TALIASQVPK EKSGSALGTL STGVVAGTLT GPFIGGFIAE LFGIRTVFLL VGSFLFLAAI
     LTICFIKEDF QPVAKEKAIP TKELFTSVKY PYLLLNLFLT SFVIQFSAQS IGPILALYVR
     DLGQTENLLF VSGLIVSSMG FSSMMSAGVM GKLGDKVGNH RLLVVAQFYS VIIYLLCANA
     SSPLQLGLYR FLFGLGTGAL IPGVNALLSK MTPKAGISRV FAFNQVFFYL GGVVGPMAGS
     AVAGQFGYHA VFYATSLCVA FSCLFNLIQF RTLLKVKEI