ID   PMRB_PSEAE              Reviewed;         477 AA.
AC   Q9HV31;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Sensor protein kinase PmrB {ECO:0000303|PubMed:14507375};
DE            EC=2.7.13.3 {ECO:0000250|UniProtKB:P40719};
GN   Name=pmrB; OrderedLocusNames=PA4777;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, AND INDUCTION BY CATIONIC ANTIMICROBIAL PEPTIDES.
RX   PubMed=14507375; DOI=10.1046/j.1365-2958.2003.03673.x;
RA   McPhee J.B., Lewenza S., Hancock R.E.;
RT   "Cationic antimicrobial peptides activate a two-component regulatory
RT   system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic
RT   antimicrobial peptides in Pseudomonas aeruginosa.";
RL   Mol. Microbiol. 50:205-217(2003).
CC   -!- FUNCTION: Member of the two-component regulatory system PmrA/PmrB that
CC       plays a role in the regulation of resistance towards polymyxin B and
CC       cationic antimicrobial peptides in response to limiting concentrations
CC       of Mg(2+). Autoregulates also its own pmrAB operon under Mg(2+)-
CC       limiting conditions (PubMed:14507375). May function as a membrane-
CC       associated protein kinase that phosphorylates PmrA in response to
CC       environmental signals leading to activation of specific gene promoters
CC       (Probable). {ECO:0000269|PubMed:14507375, ECO:0000305|PubMed:14507375}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3; Evidence={ECO:0000250|UniProtKB:P40719};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: By cationic antimicrobial peptides and by Mg(2+)-limiting
CC       conditions. {ECO:0000269|PubMed:14507375}.
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DR   EMBL; AE004091; AAG08163.1; -; Genomic_DNA.
DR   PIR; G83048; G83048.
DR   RefSeq; NP_253465.1; NC_002516.2.
DR   RefSeq; WP_003112923.1; NZ_QZGE01000018.1.
DR   AlphaFoldDB; Q9HV31; -.
DR   SMR; Q9HV31; -.
DR   STRING; 287.DR97_2121; -.
DR   PaxDb; Q9HV31; -.
DR   PRIDE; Q9HV31; -.
DR   EnsemblBacteria; AAG08163; AAG08163; PA4777.
DR   GeneID; 881841; -.
DR   KEGG; pae:PA4777; -.
DR   PATRIC; fig|208964.12.peg.5004; -.
DR   PseudoCAP; PA4777; -.
DR   HOGENOM; CLU_000445_89_37_6; -.
DR   InParanoid; Q9HV31; -.
DR   OMA; IRIAMRP; -.
DR   PhylomeDB; Q9HV31; -.
DR   BioCyc; PAER208964:G1FZ6-4890-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IDA:PseudoCAP.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Membrane; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix;
KW   Two-component regulatory system.
FT   CHAIN           1..477
FT                   /note="Sensor protein kinase PmrB"
FT                   /id="PRO_0000449422"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          186..238
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          246..459
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   REGION          455..477
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         249
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   477 AA;  52706 MW;  687FFF838D1E2087 CRC64;
     MSRAAVPSVR RRLLVNLLVG FVLCWLSVAA LTYHLSLKQV NRLFDDDMVD FGEAALRLLD
     LATEDQAGED GSITEIIERS REAIQGLPLL RRESALGYAL WRDGQPLLSS LNLPPEITAQ
     GPGFSTVEAQ GTHWRVLQLN IDGFQIWISE NLIYRQHTMN LLLFYSLFPL LLALPLLGGL
     VWFGVARGLA PLREVQAEVQ QRSARHLQPI AVEAVPLEIR GLIDELNLLL ERLRTALEAE
     RRLTSDAAHE IRTPLASLRT HAQVALRSED PKAHARGLLQ VSRSVERIST LMEQILLLAR
     LDGDALLEQF HPVNLATLAE DVLSELARQA IDKDIELSLH QETVYVMGID LWLKAMVGNL
     VGNALRYTPA GGQVEIRVEN RAQHAVLRVR DNGPGVALEE QQAIFTRFYR SPATSSGEGS
     GLGLPIVKRI VELHFGSIGL GKGLEGKGLE VQVFLPKTQP DATRPPARGP DSGRSHI