AT18E_ARATH
ID AT18E_ARATH Reviewed; 374 AA.
AC Q9FHK8;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Autophagy-related protein 18e;
DE Short=AtATG18e;
GN Name=ATG18E; OrderedLocusNames=At5g05150; ORFNames=K2A11.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=15860012; DOI=10.1111/j.1365-313x.2005.02397.x;
RA Xiong Y., Contento A.L., Bassham D.C.;
RT "AtATG18a is required for the formation of autophagosomes during nutrient
RT stress and senescence in Arabidopsis thaliana.";
RL Plant J. 42:535-546(2005).
CC -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC Required for autophagy (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex at least
CC composed of ATG18, SAC/FIG4, FAB1 and VAC14. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Note=Peripheral membrane protein of pre-autophagosomal structure (PAS)
CC and vacuole. {ECO:0000250}.
CC -!- DOMAIN: The first protein part may form a beta-propeller domain
CC involved in specific binding to phosphatidylinositol 3,5-bisphosphate
CC (PIP2), leading to the association of the protein to the membrane.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR EMBL; AB018111; BAB09691.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90834.1; -; Genomic_DNA.
DR EMBL; DQ056670; AAY78816.1; -; mRNA.
DR RefSeq; NP_196134.1; NM_120597.2.
DR AlphaFoldDB; Q9FHK8; -.
DR SMR; Q9FHK8; -.
DR STRING; 3702.AT5G05150.1; -.
DR PaxDb; Q9FHK8; -.
DR PRIDE; Q9FHK8; -.
DR EnsemblPlants; AT5G05150.1; AT5G05150.1; AT5G05150.
DR GeneID; 830397; -.
DR Gramene; AT5G05150.1; AT5G05150.1; AT5G05150.
DR KEGG; ath:AT5G05150; -.
DR Araport; AT5G05150; -.
DR TAIR; locus:2156779; AT5G05150.
DR eggNOG; KOG2111; Eukaryota.
DR HOGENOM; CLU_025895_2_1_1; -.
DR InParanoid; Q9FHK8; -.
DR OMA; LEHKHFF; -.
DR OrthoDB; 1216824at2759; -.
DR PhylomeDB; Q9FHK8; -.
DR PRO; PR:Q9FHK8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FHK8; baseline and differential.
DR Genevisible; Q9FHK8; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IBA:GO_Central.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0006497; P:protein lipidation; IBA:GO_Central.
DR GO; GO:0034497; P:protein localization to phagophore assembly site; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Membrane; Protein transport; Reference proteome; Repeat;
KW Transport; Vacuole; WD repeat.
FT CHAIN 1..374
FT /note="Autophagy-related protein 18e"
FT /id="PRO_0000421883"
FT REPEAT 28..66
FT /note="WD 1"
FT REPEAT 72..117
FT /note="WD 2"
FT REPEAT 202..242
FT /note="WD 3"
FT REPEAT 247..286
FT /note="WD 4"
SQ SEQUENCE 374 AA; 41669 MW; 5ECBC0155331063E CRC64;
MNSIVSTVRG ILTLSRSETF DSPRDEAKSD LKVLSVAWNQ VCSGFIVGTN HGFNVYSCKP
MIKKSISRAP HESGFKVAEM LFLSNLFAFV GNGYNNSEYP PNKVFVWDDY RNCCLSELTF
KSEVIAVKLA REHVVVVLKQ NIYVYTFNNL KVDRVIETLM NPKGLCCVTH VESKAVLACP
GFHPGQVQVH DLRWNVIKFI KAHDSAIACM TLTLDGSLLA TASTKGTLIR IFNAVDGTLL
QEFRRGVERA EIYNVAISSN LKWVAASSEK GTLHVFRLRP DILSFDPASS SSFIRVILPK
YLYENERSFA QFSLPASTKF IVGFGSENTV LLVGIDGSFR RCKFDHADGG QMVELEHKYF
FSLQETGNTM VGGV
