ID   PMT1_ARATH              Reviewed;         611 AA.
AC   Q8H118; Q93VT5; Q9LW67;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Probable methyltransferase PMT1;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=At3g23300; ORFNames=MLM24.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 236-611.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=17425712; DOI=10.1111/j.1365-313x.2007.03086.x;
RA   Mouille G., Ralet M.C., Cavelier C., Eland C., Effroy D., Hematy K.,
RA   McCartney L., Truong H.N., Gaudon V., Thibault J.F., Marchant A., Hofte H.;
RT   "Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-
RT   localized protein with a putative methyltransferase domain.";
RL   Plant J. 50:605-614(2007).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA   Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT   "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT   methyltransferase is required for cell adhesion and co-ordinated plant
RT   development.";
RL   Plant J. 50:735-750(2007).
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Co-expressed with the galacturonosyltransferases
CC       GAUT8/QUASIMODO1 and GAUT9.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB015474; BAB02273.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE76749.1; -; Genomic_DNA.
DR   EMBL; CP002686; ANM65095.1; -; Genomic_DNA.
DR   EMBL; AY035138; AAK59642.2; -; mRNA.
DR   EMBL; BT000890; AAN41290.1; -; mRNA.
DR   RefSeq; NP_001327090.1; NM_001338623.1.
DR   RefSeq; NP_566725.2; NM_113232.5.
DR   AlphaFoldDB; Q8H118; -.
DR   BioGRID; 7242; 1.
DR   IntAct; Q8H118; 1.
DR   PaxDb; Q8H118; -.
DR   PRIDE; Q8H118; -.
DR   ProteomicsDB; 234785; -.
DR   EnsemblPlants; AT3G23300.1; AT3G23300.1; AT3G23300.
DR   EnsemblPlants; AT3G23300.2; AT3G23300.2; AT3G23300.
DR   GeneID; 821910; -.
DR   Gramene; AT3G23300.1; AT3G23300.1; AT3G23300.
DR   Gramene; AT3G23300.2; AT3G23300.2; AT3G23300.
DR   KEGG; ath:AT3G23300; -.
DR   Araport; AT3G23300; -.
DR   TAIR; locus:2090935; AT3G23300.
DR   eggNOG; ENOG502QTWS; Eukaryota.
DR   HOGENOM; CLU_010485_2_2_1; -.
DR   InParanoid; Q8H118; -.
DR   OMA; WRTSESF; -.
DR   OrthoDB; 288023at2759; -.
DR   PhylomeDB; Q8H118; -.
DR   PRO; PR:Q8H118; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q8H118; baseline and differential.
DR   Genevisible; Q8H118; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005768; C:endosome; HDA:TAIR.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR   GO; GO:0005797; C:Golgi medial cisterna; HDA:TAIR.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; HDA:TAIR.
DR   GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR   GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004159; Put_SAM_MeTrfase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR10108; PTHR10108; 1.
DR   Pfam; PF03141; Methyltransf_29; 1.
DR   SUPFAM; SSF53335; SSF53335; 2.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Golgi apparatus; Membrane; Methyltransferase;
KW   Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..611
FT                   /note="Probable methyltransferase PMT1"
FT                   /id="PRO_0000393241"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        12..32
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..611
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        345
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   611 AA;  69356 MW;  4043796531723BEC CRC64;
     MRGRSEGGKK KPVIVLLCVA SVVLVFVYLF FGSSNHKAIE YGRKLGLGGD DDDSTKKDDT
     SSSFYVEDVV GNGFTPRSFP VCDDRHSELI PCLDRNLIYQ MRLKLDLSLM EHYERHCPPP
     ERRFNCLIPP PPGYKIPIKW PKSRDEVWKV NIPHTHLAHE KSDQNWMVVK GEKINFPGGG
     THFHYGADKY IASMANMLNF PNNVLNNGGR LRTFLDVGCG VASFGGYLLA SEIMTMSLAP
     NDVHQNQIQF ALERGIPAYL GVLGTKRLPY PSRSFELAHC SRCRIDWLQR DGILLLELDR
     VLRPGGYFAY SSPEAYAQDE EDLRIWREMS ALVGRMCWTI AAKRNQTVIW QKPLTNDCYL
     GREPGTQPPL CNSDSDPDAV YGVNMEACIT QYSDHDHKTK GSGLAPWPAR LTSPPPRLAD
     FGYSTDIFEK DTETWRQRVD TYWDLLSPKI QSDTVRNIMD MKASMGSFAA ALKEKDVWVM
     NVVPEDGPNT LKLIYDRGLM GAVHSWCEAF STYPRTYDLL HAWDIISDIK KRGCSAEDLL
     LEMDRILRPS GFILIRDKQS VVDLVKKYLK ALHWEAVETK TASESDQDSD NVILIVQKKL
     WLTSESLRDL E