PMT2_ARATH
ID PMT2_ARATH Reviewed; 616 AA.
AC B9DFI7; Q94C19; Q9ZVH2;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Probable methyltransferase PMT2;
DE EC=2.1.1.-;
GN OrderedLocusNames=At1g26850; ORFNames=T2P11.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY.
RX PubMed=17425712; DOI=10.1111/j.1365-313x.2007.03086.x;
RA Mouille G., Ralet M.C., Cavelier C., Eland C., Effroy D., Hematy K.,
RA McCartney L., Truong H.N., Gaudon V., Thibault J.F., Marchant A., Hofte H.;
RT "Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-
RT localized protein with a putative methyltransferase domain.";
RL Plant J. 50:605-614(2007).
RN [6]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B9DFI7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B9DFI7-2; Sequence=VSP_038900;
CC -!- MISCELLANEOUS: Co-expressed with the galacturonosyltransferases GAUT1
CC and GAUT9.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AC005508; AAD14491.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30749.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30751.1; -; Genomic_DNA.
DR EMBL; AY037230; AAK59830.1; -; mRNA.
DR EMBL; BT000640; AAN18206.1; -; mRNA.
DR EMBL; AK316786; BAH19504.1; -; mRNA.
DR PIR; C86395; C86395.
DR RefSeq; NP_564265.1; NM_102449.4. [B9DFI7-1]
DR RefSeq; NP_849710.1; NM_179379.4. [B9DFI7-1]
DR AlphaFoldDB; B9DFI7; -.
DR BioGRID; 24465; 4.
DR IntAct; B9DFI7; 3.
DR STRING; 3702.AT1G26850.2; -.
DR iPTMnet; B9DFI7; -.
DR SwissPalm; B9DFI7; -.
DR PaxDb; B9DFI7; -.
DR PRIDE; B9DFI7; -.
DR ProteomicsDB; 234682; -. [B9DFI7-1]
DR EnsemblPlants; AT1G26850.1; AT1G26850.1; AT1G26850. [B9DFI7-1]
DR EnsemblPlants; AT1G26850.2; AT1G26850.2; AT1G26850. [B9DFI7-1]
DR GeneID; 839229; -.
DR Gramene; AT1G26850.1; AT1G26850.1; AT1G26850. [B9DFI7-1]
DR Gramene; AT1G26850.2; AT1G26850.2; AT1G26850. [B9DFI7-1]
DR KEGG; ath:AT1G26850; -.
DR Araport; AT1G26850; -.
DR TAIR; locus:2202805; AT1G26850.
DR eggNOG; ENOG502QPR2; Eukaryota.
DR HOGENOM; CLU_010485_2_2_1; -.
DR InParanoid; B9DFI7; -.
DR OMA; KPCHPRY; -.
DR PhylomeDB; B9DFI7; -.
DR PRO; PR:B9DFI7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; B9DFI7; baseline and differential.
DR Genevisible; B9DFI7; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005797; C:Golgi medial cisterna; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Golgi apparatus; Membrane;
KW Methyltransferase; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..616
FT /note="Probable methyltransferase PMT2"
FT /id="PRO_0000393242"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..616
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 205
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 611
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 517..542
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038900"
FT CONFLICT 123
FT /note="E -> K (in Ref. 4; BAH19504)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 616 AA; 69623 MW; 51086D43592B1FBE CRC64;
MALKSSSADG KTRSSVQIFI VFSLCCFFYI LGAWQRSGFG KGDSIALEMT NSGADCNIVP
SLNFETHHAG ESSLVGASEA AKVKAFEPCD GRYTDYTPCQ DQRRAMTFPR DSMIYRERHC
APENEKLHCL IPAPKGYVTP FSWPKSRDYV PYANAPYKAL TVEKAIQNWI QYEGDVFRFP
GGGTQFPQGA DKYIDQLASV IPMENGTVRT ALDTGCGVAS WGAYLWSRNV RAMSFAPRDS
HEAQVQFALE RGVPAVIGVL GTIKLPYPTR AFDMAHCSRC LIPWGANDGM YLMEVDRVLR
PGGYWILSGP PINWKVNYKA WQRPKEDLQE EQRKIEEAAK LLCWEKKYEH GEIAIWQKRV
NDEACRSRQD DPRANFCKTD DTDDVWYKKM EACITPYPET SSSDEVAGGE LQAFPDRLNA
VPPRISSGSI SGVTVDAYED DNRQWKKHVK AYKRINSLLD TGRYRNIMDM NAGFGGFAAA
LESQKLWVMN VVPTIAEKNR LGVVYERGLI GIYHDWCEAF STYPRTYDLI HANHLFSLYK
NKCNADDILL EMDRILRPEG AVIIRDDVDT LIKVKRIIAG MRWDAKLVDH EDGPLVPEKV
LIAVKQYWVT NSTSTH
