PMT4_ARATH
ID PMT4_ARATH Reviewed; 603 AA.
AC Q8GYW9; C0Z2E2; Q3EDD1; Q9LMH1;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Probable methyltransferase PMT4;
DE EC=2.1.1.-;
GN OrderedLocusNames=At1g13860; ORFNames=F16A14.2, F16A14.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8GYW9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8GYW9-2; Sequence=VSP_038901;
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79416.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAH56871.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC068197; AAF79416.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29074.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29075.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29076.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29077.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59945.1; -; Genomic_DNA.
DR EMBL; AK117344; BAC42014.1; -; mRNA.
DR EMBL; AK318756; BAH56871.1; ALT_FRAME; mRNA.
DR PIR; G86271; G86271.
DR RefSeq; NP_001322262.1; NM_001332093.1. [Q8GYW9-1]
DR RefSeq; NP_172839.1; NM_101252.3. [Q8GYW9-1]
DR RefSeq; NP_849656.2; NM_179325.2. [Q8GYW9-2]
DR RefSeq; NP_849657.1; NM_179326.3. [Q8GYW9-1]
DR RefSeq; NP_973819.1; NM_202090.1. [Q8GYW9-1]
DR AlphaFoldDB; Q8GYW9; -.
DR STRING; 3702.AT1G13860.1; -.
DR PaxDb; Q8GYW9; -.
DR PRIDE; Q8GYW9; -.
DR ProteomicsDB; 234684; -. [Q8GYW9-1]
DR EnsemblPlants; AT1G13860.1; AT1G13860.1; AT1G13860. [Q8GYW9-1]
DR EnsemblPlants; AT1G13860.2; AT1G13860.2; AT1G13860. [Q8GYW9-2]
DR EnsemblPlants; AT1G13860.3; AT1G13860.3; AT1G13860. [Q8GYW9-1]
DR EnsemblPlants; AT1G13860.4; AT1G13860.4; AT1G13860. [Q8GYW9-1]
DR EnsemblPlants; AT1G13860.5; AT1G13860.5; AT1G13860. [Q8GYW9-1]
DR GeneID; 837945; -.
DR Gramene; AT1G13860.1; AT1G13860.1; AT1G13860. [Q8GYW9-1]
DR Gramene; AT1G13860.2; AT1G13860.2; AT1G13860. [Q8GYW9-2]
DR Gramene; AT1G13860.3; AT1G13860.3; AT1G13860. [Q8GYW9-1]
DR Gramene; AT1G13860.4; AT1G13860.4; AT1G13860. [Q8GYW9-1]
DR Gramene; AT1G13860.5; AT1G13860.5; AT1G13860. [Q8GYW9-1]
DR KEGG; ath:AT1G13860; -.
DR Araport; AT1G13860; -.
DR TAIR; locus:2014789; AT1G13860.
DR eggNOG; ENOG502QT31; Eukaryota.
DR HOGENOM; CLU_010485_2_3_1; -.
DR InParanoid; Q8GYW9; -.
DR OMA; LREFPLC; -.
DR OrthoDB; 359485at2759; -.
DR PhylomeDB; Q8GYW9; -.
DR PRO; PR:Q8GYW9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GYW9; baseline and differential.
DR Genevisible; Q8GYW9; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Methyltransferase; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..603
FT /note="Probable methyltransferase PMT4"
FT /id="PRO_0000393244"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..603
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 393
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..156
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038901"
FT CONFLICT 101
FT /note="D -> G (in Ref. 3; BAC42014)"
FT /evidence="ECO:0000305"
FT CONFLICT 419
FT /note="I -> T (in Ref. 3; BAC42014)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 603 AA; 68001 MW; 9F223AF28B57F77A CRC64;
MKVASVIGLR PRISGLLFLT LGVIALITIL VPNSDSSSTT STTRVPPSNI YSNYGRVKEQ
AAVDYLDLRF FSLGVNRLKE FPLCGKERDN YVPCYNVTES DRNCEFAREE ERCLVRPPRD
YKIPLRWPVG RDIIWTGNVK ITKDQFLSSG TMTKRLMLLE ENQITFHSDD GLIFDGVKDY
AFQIAEMIGL GSDTEFPQAG IRTVLDIGCG FGSFGAHLVS LNVMPICIAE YETSGSQVQL
ALERGLPAMI GNFFSKQLPY PALSFDMVHC AQCGITWDIK DAMLLLEVDR VLKPGGYFVL
TSPTSKAQGN SPDTKKTSIS TRVDELSKKI CWSLSGQQDE TFLWQKTADP NCYSSRSQAS
IPVCKDDDSV PYYHPLVPCI SGTKSKRWIP IQNRSRASGT SLSELEIHGI KPEEFDEDIQ
VWRSALKNYW SLLTPLIFSD HPKRPGDEDP VPPFYMIRNA MDMNARYGNL NQALLNQGKS
VWVMNVVPVK ARNTLPIILD RGFTGALHDW CEPFPTYPRT YDMLHANELL THLSSERCSL
MDLFLEMDRI LRPEGWVVLS DKLGVIEMAR TLAARVRWEA RVIDIQDGSD QRLLVCQKPL
LKK
