PMT5_ARATH
ID PMT5_ARATH Reviewed; 606 AA.
AC Q3EC77; Q8H0Z7; Q94BV9; Q9ZQ84;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable methyltransferase PMT5;
DE EC=2.1.1.-;
GN OrderedLocusNames=At2g03480; ORFNames=T4M8.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT methyltransferase is required for cell adhesion and co-ordinated plant
RT development.";
RL Plant J. 50:735-750(2007).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3EC77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3EC77-2; Sequence=VSP_038902;
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD17428.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAK63953.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=AAN46794.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AC006284; AAD17428.2; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05705.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05706.1; -; Genomic_DNA.
DR EMBL; AY039849; AAK63953.1; ALT_SEQ; mRNA.
DR EMBL; BT001040; AAN46794.1; ALT_SEQ; mRNA.
DR PIR; A84449; A84449.
DR RefSeq; NP_027543.2; NM_126399.3. [Q3EC77-1]
DR RefSeq; NP_973410.1; NM_201681.2. [Q3EC77-2]
DR AlphaFoldDB; Q3EC77; -.
DR SMR; Q3EC77; -.
DR STRING; 3702.AT2G03480.1; -.
DR PaxDb; Q3EC77; -.
DR PRIDE; Q3EC77; -.
DR ProteomicsDB; 234984; -. [Q3EC77-1]
DR EnsemblPlants; AT2G03480.1; AT2G03480.1; AT2G03480. [Q3EC77-1]
DR EnsemblPlants; AT2G03480.2; AT2G03480.2; AT2G03480. [Q3EC77-2]
DR GeneID; 814877; -.
DR Gramene; AT2G03480.1; AT2G03480.1; AT2G03480. [Q3EC77-1]
DR Gramene; AT2G03480.2; AT2G03480.2; AT2G03480. [Q3EC77-2]
DR KEGG; ath:AT2G03480; -.
DR Araport; AT2G03480; -.
DR TAIR; locus:2063798; AT2G03480.
DR eggNOG; ENOG502QT31; Eukaryota.
DR InParanoid; Q3EC77; -.
DR OMA; DGDSAPY; -.
DR OrthoDB; 359485at2759; -.
DR PhylomeDB; Q3EC77; -.
DR PRO; PR:Q3EC77; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q3EC77; baseline and differential.
DR Genevisible; Q3EC77; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004159; Put_SAM_MeTrfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10108; PTHR10108; 1.
DR Pfam; PF03141; Methyltransf_29; 2.
DR SUPFAM; SSF53335; SSF53335; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Methyltransferase; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..606
FT /note="Probable methyltransferase PMT5"
FT /id="PRO_0000393245"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..606
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 409
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 402..426
FT /note="KRWISIQNRSAVAGTTSAGLEIHGK -> LKPEEFFEDTQIWR (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038902"
SQ SEQUENCE 606 AA; 67819 MW; B2797F86E6B05EBB CRC64;
MRGSWYKSVS SVFGLRPRIR GLLFFIVGVV ALVTILAPLT SNSYDSSSSS TLVPNIYSNY
RRIKEQAAVD YLDLRSLSLG ASLKEFPFCG KERESYVPCY NITGNLLAGL QEGEELDRHC
EFEREKERCV VRPPRDYKIP LRWPLGRDII WSGNVKITKD QFLSSGTVTT RLMLLEENQI
TFHSEDGLVF DGVKDYARQI AEMIGLGSDT EFAQAGVRTV LDIGCGFGSF GAHLVSLKLM
PICIAEYEAT GSQVQLALER GLPAMIGNFF SKQLPYPALS FDMVHCAQCG TTWDIKDAML
LLEVDRVLKP GGYFVLTSPT NKAQGNLPDT KKTSISTRVN ELSKKICWSL TAQQDETFLW
QKTSDSSCYS SRSQASIPLC KDGDSVPYYH PLVPCISGTT SKRWISIQNR SAVAGTTSAG
LEIHGKSALK NYWSLLTPLI FSDHPKRPGD EDPLPPFNMI RNVMDMHARF GNLNAALLDE
GKSAWVMNVV PVNARNTLPI ILDRGFAGVL HDWCEPFPTY PRTYDMLHAN ELLTHLSSER
CSLMDLFLEM DRILRPEGWV VLSDKVGVIE MARALAARVR WEARVIDLQD GSDQRLLVCQ
KPFIKK
